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Isolation and characterization of a potentially virulent species Entamoeba nuttalli from captive Japanese macaques

Published online by Cambridge University Press:  27 July 2009

H. TACHIBANA ,
T. YANAGI ,
A. AKATSUKA ,
S. KOBAYASHI ,
H. KANBARA  and
V. TSUTSUMI
H. TACHIBANA*
Affiliation:
Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan
T. YANAGI
Affiliation:
Animal Research Center for Tropical Infections, Nagasaki University, Nagasaki 852-8523, Japan
A. AKATSUKA
Affiliation:
Teaching and Research Support Center, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan
S. KOBAYASHI
Affiliation:
Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan
H. KANBARA
Affiliation:
Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan
V. TSUTSUMI
Affiliation:
Department of Infectomic and Molecular Pathogenesis, Center for Research and Advanced Studies, National Polytechnic Institute, Mexico City 07360, Mexico Research Center for Innovative Cancer Therapy, Kurume University School of Medicine, Kurume 830-0011, Japan
*
*Corresponding author: Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan. Tel: +81 463 93 1121. Fax: +81 463 95 5450. E-mail: [email protected]
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Summary

We have recently proposed revival of the name Entamoeba nuttalliCastellani, 1908 for a virulent amoeba (P19-061405 strain) isolated from a rhesus monkey (Macaca mulatta) and located phylogenetically between E. histolytica and E. dispar. In this study, E. nuttalli was isolated from feces of captive Japanese macaques (M. fuscata) in an open-air corral in Japan. The sequence of the 18S rRNA gene in the isolates differed from the P19-061405 strain in 2 nucleotide positions, but was identical to the EHMfas1 strain isolated previously from a cynomolgus monkey (M. fascicularis). One of the E. nuttalli isolates from Japanese macaques, named the NASA6 strain, was axenized and cloned. In isoenzyme analysis, the mobilities of hexokinase and phosphate glucose isomerase in the NASA6 strain were identical to those in the P19-061405 and EHMfas1 strains, but the mobility of phosphoglucomutase was different. These results were supported by gene analyses of these enzymes. Inoculation of NASA6 strain trophozoites into the liver of hamsters led to formation of an amoebic liver abscess. The liver lesions were characterized by extensive necrosis associated with inflammatory reactions. These results demonstrate that the NASA6 strain is potentially virulent and that E. nuttalli should be recognized as a common parasite in macaques.

Keywords

Entamoeba nuttalliJapanese macaque18S rRNA genevirulencyzymodeme
Type
Research Article
Information
Parasitology , Volume 136 , Issue 10 , September 2009 , pp. 1169 - 1177
Copyright
Copyright © Cambridge University Press 2009

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