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Investigation of the chemosensory function of amphids of Syngamus trachea using electrophysiological techniques

Published online by Cambridge University Press:  06 April 2009

E. Riga ,
R. N. Perry ,
J. Barrett  and
M. R. L. Johnston
E. Riga
Affiliation:
Entomology and Nematology Department, IACR-Rothatnsted, Harpenden, Herts. AL5 2JQ Institute of Biological Sciences, University of Wales, Aberystwyth, Dyfed SY23 3D A
R. N. Perry
Affiliation:
Entomology and Nematology Department, IACR-Rothatnsted, Harpenden, Herts. AL5 2JQ
J. Barrett
Affiliation:
Institute of Biological Sciences, University of Wales, Aberystwyth, Dyfed SY23 3D A
M. R. L. Johnston
Affiliation:
Institute of Biological Sciences, University of Wales, Aberystwyth, Dyfed SY23 3D A
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Summary

Syngamus trachea, the gape nematode, has been used as a model to study the chemosensory function of amphids. Extracellular electrophysiological recordings were performed directly on amphids. The amphids were stimulated by blood serum from a host bird and by D-tryptophan. The spike frequency produced by the amphid increased significantly after the application of the serum and the application of D-tryptophan. Two types of amphidial neurones responded to the presence of the stimuli. These extracellular recordings have verified the chemosensory function of the amphids.

Keywords

Syngamus tracheaamphidselectrophysiological recordingsspikes
Type
Research Article
Information
Parasitology , Volume 111 , Issue 3 , September 1995 , pp. 347 - 351
Copyright
Copyright © Cambridge University Press 1995

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References

REFERENCES

Bargmann, C. I., Thomas, J. H. & Horvitz, H. R. (1990). Chemosensory cell function in the behaviour and development of Caenorhabditis elegans. Cold Spring Harbor Symposia on Quantitative Biology 55, 529–38.CrossRefGoogle ScholarPubMed
Coomans, A. (1979). The anterior sensilla of nematodes. Revue de Nématologie 2, 259–83.Google Scholar
Coomans, A. & De Grisse, A. (1981). Sensory structures. In Plant Parasitic Nematodes, Vol. 3 (ed. Zuckerman, B. M. & Rohde, R. A.), pp. 127–174. New York: Academic Press.CrossRefGoogle Scholar
Dusenbery, D. B. (1975). The avoidance of D-tryptophan by the nematode Caenorhabditis elegans. Journal of Experimental Zoology 193, 413 17.CrossRefGoogle ScholarPubMed
Jones, J. T., Perry, R. N. & Johnston, M. R. L. (1991). Electrophysiological recordings of electrical activity and responses to stimulants from Globodera rostochiensis and Syngamus trachea. Revue de Nématologie 14, 467–73.Google Scholar
Kaplan, J. M. & Horvitz, H. R. (1993). A dual mechanosensory and chemosensory neurone in Caenorhabditis elegans. Proceedings of the National Academy of Sciences, USA 90, 2227–31.CrossRefGoogle ScholarPubMed
McLaren, D. J. (1976). Nematode sense organs. Advances in Parasitology 14, 195–265.CrossRefGoogle ScholarPubMed
Perry, R. N. (1994). Studies on nematode sensory perception as a basis for novel control strategies. Fundamental and Applied Nematology 17, 199–202.Google Scholar
Sokal, R. R. & Rohlf, F. J. (1969). Biometry. San Francisco: W. H. Freeman and Company.Google Scholar
Stewart, G. R., Perry, R. N., Alexander, J. & Wright, D. J. (1993). A glycoprotein specific to the amphids of Meloidogyne species. Parasitology 106, 405–12.CrossRefGoogle Scholar
Stewart, G. R., Perry, R. N. & Wright, D. J. (1993). Studies on the amphid specific glycoprotein gp32 in different life-cycle stages of Meloidogyne species. Parasitology 107, 573–8.CrossRefGoogle Scholar
Trett, M. W. & Perry, R. N. (1985). Functional and evolutionary implications of the anterior sensory anatomy of species of root lesion nematodes (genus Pratylenchus). Revue de Nématologie 8, 341–55.Google Scholar
Wadhams, L. J., Angst, M. E. & Blight, M. M. (1982). Responses of the olfactory receptors of Scolytus scolytus (F.) (Coleoptera: Scolytidae) to the stereoisomers of 4-methyl-3-heptanol. Journal of Chemical Ecology 8, 477–92.CrossRefGoogle Scholar
Wright, K. A. (1983). Nematode chemosensilla: form and function. Journal of Nematology 15, 151–8.Google ScholarPubMed