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Invasion of skin by larvae of the cat hookworm, Ancylostoma tubaeforme

Published online by Cambridge University Press:  06 April 2009

B. E. Matthews
B. E. Matthews
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London University, London S.W. 7
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Extract

A modification of the ‘floating raft’ method of in vitro penetration study is described. This allows replication of results and additional conveniences in operation. Filter paper membranes have shown characteristics similar to those of skin and have been used for quantitative studies. A method of digesting penetrating larvae from skin has been used to reduce the variability inherent in whole skin. Neither chloroform soluble skin products nor gravity were found to have a significant effect on the number of larvae entering membranes. Exsheathment of larvae was not essential prior to penetration and no specific stimuli for exsheathment appear to be necessary. Scanning electron micrographs have shown that entry may be by either hair follicles or desquamations of the stratum corneum. No evidence of enzymic activity during the invasion process has been found so far and the results suggest that a mechanical rather than chemical system obtains.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 3 , December 1972 , pp. 457 - 467
Copyright
Copyright © Cambridge University Press 1972

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References

Barrett, J. (1969). The effect of ageing on the metabolism of infective larvae of Strongyloides ratti Sandground, 1925. Parasitology 59, 3–17.CrossRefGoogle Scholar
Bettley, F. R. & Donoghue, E. (1960). Effect of soap on the diffusion of water through isolated human epidermis. Nature, Lond. 185, 17–20.CrossRefGoogle ScholarPubMed
Billingham, R. E., & Medawar, P. B., (1951). The viability of mammalian skin after freezing, thawing and freeze-drying. In Freezing and Drying (ed. Harris, R. J. C.), pp. 55–62. Symposium, Institute of Biology.Google Scholar
Bird, A. F., & Bird, J., (1969). Skeletal structures and integument of Acanthocephala and Nematoda. In Chemical Zoology (ed. Florkin, M. and Scheer, B. T.), vol. III, pp. 253–88. New York: Academic Press.CrossRefGoogle Scholar
Clegg, J. A. (1969). Skin penetration by cercariae of the bird schistosome Austrobilharzia terrigalensis: the stimulatory effect of cholesterol. Parasitology 59, 973–89.CrossRefGoogle ScholarPubMed
Croll, N. A. (1972 a). Energy utilization of infective Ancylostoma tubaeforme larvae. Parasitology 64, 355–68.CrossRefGoogle ScholarPubMed
Croll, N. A. (1972 b). Feeding and lipid synthesis of Ancylostoma tubaeforme preinfective larvae. Parasitology 64, 369–78.CrossRefGoogle ScholarPubMed
Croll, N. A. (1972 c). Behaviour of larval nematodes. Symposium on Behavioural Aspects of Parasite Transmission. Linnaean Society (in the Press).Google Scholar
Goodey, T. (1922). Observations on the ensheathed larvae of some parasitic nematodes. Annals of Applied Biology 9, 33–48.CrossRefGoogle Scholar
Goodey, T. (1925). Observations on certain conditions requisite for skin penetration by the infective larvae of strongyloides and ankylostomes. Journal of Helminthology 3, 51–62.CrossRefGoogle Scholar
Kosuge, I. (1924). Histologische Untersuchungen über das Eindringen von Strongyloides stercoralis in die Haut von Versuchstieren. Arch, für Schiffs- und Tropenhygiene Bd. 28, 15–20.Google Scholar
Lane, C. (1933). The taxies of infective hookworm larvae. Annals of Tropical Medicine and Parasitology 27, 237–50.CrossRefGoogle Scholar
Lapage, G. (1935). The second ecdysis of infective nematode larvae. Parasitology 27, 186–206.CrossRefGoogle Scholar
Looss, A. (1911). The anatomy and life-history of Agchylostoma duodenale Dub. Pt. II. Cairo Records of the Egyptian Government School of Medicine.Google Scholar
Rogers, W. P. (1939). The physiological ageing of ancylostome larvae. Journal of Helminthology 17, 195–202.CrossRefGoogle Scholar
Rogers, W. P. & Sommerville, R. I. (1963). The infective stage of nematode parasites and its significance in parasitism. Advances in Parasitology 1, 109–77.CrossRefGoogle ScholarPubMed
Rose, G. G. (1954). A separable and multipurpose tissue culture chamber. Texas Reports of Biology and Medicine 12, 1074–83.Google ScholarPubMed
Simmonds, R. A. (1958). Studies on the sheath of fourth stage larvae of the nematode parasite Nippostrongylus muris. Experimental Parasitology 7, 14–22.CrossRefGoogle ScholarPubMed
Stirewalt, M. A., (1971). Penetration stimuli for schistosome cercariae. In Aspects of the Biology of Symbiosis (ed. Cheng, T. C.), pp. 1–24. Baltimore: University Park Press.Google Scholar
Stirewalt, M. A., Minnick, D. R. & Fregeau, W. A. (1966). Definition and collection in quantity of schistosomules of Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 352–60.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. & UY, A. (1969). Schistosoma mansoni: cercarial penetration and schistosomule collection in an in vitro system. Experimental Parasitology 26, 17–28.CrossRefGoogle Scholar
Summerlin, W. T., Charlton, M. E. & Karasek, M. A. (1970). Transplantation of organ cultures of adult human skin. Journal of Investigative Dermatology 55, 310–16.CrossRefGoogle ScholarPubMed
Trowell, O. A. (1959). The culture of mature organs in a synthetic medium. Experimental Cell Research 16, 118–47.CrossRefGoogle Scholar
Uitto, J., Lindy, S., Turto, H. & Danielson, L. (1971). Biochemical characterization of pseudoxanthoma elasticum: collagen biosynthesis in the skin. Journal of Investigative Dermatology 57, 44–48.CrossRefGoogle ScholarPubMed
Wilson, R. A., Pullin, R. & Denison, J. (1971). An investigation of the mechanism of infection by digenetic trematodes: the penetration of the miracidium of Fasciola hepatica into its snail host Lymnaea truncatula. Parasitology 63, 491–506.CrossRefGoogle ScholarPubMed