Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-20T00:04:57.975Z Has data issue: false hasContentIssue false
  • English
  • Français

Interspecific characterization of several taeniid cestodes by isoenzyme analysis using isoelectric focusing in agarose

Published online by Cambridge University Press:  06 April 2009

B. A. Allsopp ,
A. Jones ,
M. T. E. P. Allsopp ,
S. D. Newton  and
C. N. L. Macpherson
B. A. Allsopp
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, KE
A. Jones
Affiliation:
CAB International Institute of Parasitology, 395A Hatfield Road, St. Albans, Herts. AL4 0XU, England
M. T. E. P. Allsopp
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, KE
S. D. Newton
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, KE
C. N. L. Macpherson
Affiliation:
African Medical and Research Foundation, P.O. Box 30125, Nairobi, KE
Get access Rights & Permissions [Opens in a new window]

Summary

Taenia cestodes were obtained from 5 different definitive host species in Kenya and 175 different samples were examined by classical morphological methods and by isoenzyme analysis using isoelectric focusing in agarose. Gels were stained for 17 different enzymes and 3 of these were used in the construction of isoenzyme profiles. The samples fell into 25 zymodemes, and no zymodeme contained more than 1 species of Taenia, indicating that isoenzyme analysis can reliably be used for the identification of species of this genus.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 3 , December 1987 , pp. 593 - 601
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abduladze, K. I. (1964). Principles of cestodology. In Taeniata of Animals and Man and the Diseases caused by them, vol. 4, (ed. Skryabin, K. I.) pp. 530. Moscow, Izdatel 'stvo ‘Nauka’. Translated by the Israel Program for Scientific Translations, Jerusalem, 1970.Google Scholar
Allsopp, B. A. & Gibson, W. C. (1983). Iso-electric focusing in agarose, a highly discriminatory method for the detection of enzyme heterogeneity. Annals of Tropical Medicine and Parasitology 7, 169–73.CrossRefGoogle Scholar
Allsopp, B. A., Gibson, W. C. & Stagg, D. A. (1985). Characterization of some East African Theileria species isolates by isoenzyme analysis, with particular reference to T. parva. International Journal for Parasitology 15, 271–6.CrossRefGoogle ScholarPubMed
Beveridge, I. & Gregory, G. G. (1976). The identification of Taenia species from Australian carnivores. Australian Veterinary Journal 52, 369–73.CrossRefGoogle ScholarPubMed
Bursey, C. C., McKenzie, J. A. & Burt, M. D. B. (1980). Polyacrylamide gel electrophoresis in the identification of Taenia (Cestoda) by total protein. International Journal for Parasitology 10, 167–74.CrossRefGoogle Scholar
Dinnik, J. A. & Sachs, R. (1972). Taeniidae of lions in East Africa. Zeitschrift für Tropenmedizin und Parasitologie 23, 197–210.Google ScholarPubMed
Edwards, G. T. & Herbert, I. V. (1981). Some quantitative characters used in the identification of Taenia hydatigena, T. ovis, T. pisiformis, and T. multiceps adult worms, and T. multiceps metacestodes. Journal of Helminthology 55, 1–7.CrossRefGoogle Scholar
Gibson, W. C., Marshall, T. F. de C. & Godfrey, D. G. (1980). Numerical analysis of enzyme polymorphism: a new approach to the epidemiology and taxonomy of trypanosomes of the sub-genus Trypanozoon. Advances in Parasitology 18, 175–246.CrossRefGoogle Scholar
Godfrey, D. G. (1984). Molecular biochemical characterization of human parasites. Recent Advances in Tropical Medicine 1, 289–319.Google Scholar
LeRiche, P. D. & Sewell, M. M. H. (1977). Differentiation of Taenia saginata and Taenia solium by enzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 327–8.CrossRefGoogle Scholar
LeRiche, P. D. & Sewell, M. M. H. (1978). Differentiation of Taeniid cestodes by enzyme electrophoresis. International Journal for Parasitology 8, 479–83.CrossRefGoogle Scholar
Macpherson, C. N. L., French, C. M., Stevenson, P., Karstad, L. & Arundel, J. H. (1985). Hydatid disease in the Turkana district of Kenya, IV. The prevalence of Echinococcus granulosus infections in dogs and observations on the role of the dog in the lifestyle of the Turkana. Annals of Tropical Medicine and Parasitology 79, 51–61.CrossRefGoogle ScholarPubMed
Macpherson, C. N. L. & McManus, D. P. (1982). A comparative study of Echinococcus granulosus from human and animal hosts in Kenya using isoelectric focusing and isoenzyme analysis. International Journal for Parasitology 12, 515–21.CrossRefGoogle ScholarPubMed
Verster, A. (1969). A taxonomic revision of the genus Taenia Linnaeus, 1758 s.str. Onderstepoort Journal of Veterinary Research 36, 3–58.Google ScholarPubMed
Voge, M. (1969). Systematics of cestodes – present and future. In Problems in the Systematics of Parasites (ed. Schmidt, G. D.), pp. 49–73. Baltimore: University Park Press.Google Scholar