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Interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi with chicken macrophages in vitro

Published online by Cambridge University Press:  06 April 2009

M. N. L. de Meirelles ,
T. C. de Araujo Jorge  and
W. de Souza
M. N. L. de Meirelles
Affiliation:
Laboratório de Ultra-estrutura Celular, Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21.910, Rio de Janeiro, Brasil and Fundação Oswaldo Cruz, Av. Brasil, Rio de Janeiro, Brasil
T. C. de Araujo Jorge
Affiliation:
Laboratório de Ultra-estrutura Celular, Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21.910, Rio de Janeiro, Brasil and Fundação Oswaldo Cruz, Av. Brasil, Rio de Janeiro, Brasil
W. de Souza*
Affiliation:
Laboratório de Ultra-estrutura Celular, Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21.910, Rio de Janeiro, Brasil and Fundação Oswaldo Cruz, Av. Brasil, Rio de Janeiro, Brasil
*
*Dr Wanderley do Souza, Instituto de Biofísica-UFRJ, Ilha do Fundão, 21.910, Rio do Janeiro, Brasil.
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Summary

Chicken macrophages, obtained by cultivation of blood monocytes, were infected with epimastigote and bloodstream trypomastigotes of the Y and the CL strains of Trypanosoma cruzi. The percentage of infected cells and the mean number of parasites per cell were determined after 2, 6, 12 and 24 h of parasite–cell contact. After 6 h of contact about 80% and 40% of the macrophages were infected by trypomastigotes of the Y and CL strains respectively. After longer periods of contact almost all macrophages were infected by Y trypomastigotes while only about 60% were infected by those of the CL strain. After 2 h of contact almost all macrophages were infected by CL epimastigotes while only about 50% were infected by Y epimastigotes. After 6 h of contact almost all macrophages were infected by epimastigotes of both strains. These results are discussed taking into consideration differences between parasites of the two strains and between the two developmental stages of the T. cruzi life-cycle.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 2 , October 1980 , pp. 373 - 381
Copyright
Copyright © Cambridge University Press 1980

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References

REFERENCES

Alcantara, A. & Brener, Z. (1978). The in vitro interaction of Trypanosoma cruzi bloodstream forms and mouse peritoneal macrophages. Acta tropica 35, 209–19.Google ScholarPubMed
Alexander, J. (1975). Effect of the antiphagocytic agent cytochalasin B on macrophage invasion by Leishmania mexicana promastigotes and Trypanosoma cruzi epimastigotes. Journal of Protozoology 22, 237–40.CrossRefGoogle ScholarPubMed
Andrade, S. G. (1974). Caracterização do cepas do Trypanosoma Cruzi isoladas do recôncavo baiano. Revista de Patolo gia Tropical 3, 65–121.Google Scholar
Behbehani, K. (1973). Developmental cycles of Trypanosoma (Schizotrypanum) cruzi (Chagas, 1909) in mouse peritoneal macrophages in vitro. Parasitology 66, 343–53.CrossRefGoogle ScholarPubMed
Bittencourt, A. L. (1976). Congenital Chagas disease. American Journal of Diseases of Children 130, 97–103.Google ScholarPubMed
Brener, Z. (1965). Comparative studies of different strains of Trypanosoma cruzi. Annals of Tropical Medicine and Parasitology 59, 19–26.CrossRefGoogle ScholarPubMed
Brener, Z. (1969). The behaviour of slender and stout forms of Trypanosoma cruzi in the bloodstream of normal and immune mice. Annals of Tropical Medicine and Parasitology 63, 215–20.CrossRefGoogle ScholarPubMed
Brener, Z. (1973). Biology of Trypanosoma cruzi. Annual Review of Microbiology 27, 347–82.CrossRefGoogle ScholarPubMed
Brener, Z. & Chiari, E. (1963). Variações morfológicas observadas em diferentes amostras de Trypanosoma cruzi. Revista do Instituto de Medicina Tropical de São Paulo 5, 220–4.Google Scholar
Camargo, E. P. (1964). Growth and differentiation in Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media. Revista do Instituto de Medicina Tropical de São Paulo 6, 93–100.Google ScholarPubMed
De Souza, W., Arguello, C., Martinez-Palomo, A., Trissl, D., Gonzales-Robles, A. & Chiari, E. (1977). Surface charge of Trypanosoma cruzi. Binding of cationized ferritin and measurement of cellular electrophoretic mobility. Journal of Protozoology 24, 411–15.CrossRefGoogle ScholarPubMed
De Souza, W., Martinez-Palomo, A. & Gonzales-Robles, A. (1978). The cell surface of Trypanosoma cruzi: cytochemistry and freeze-fracture. Journal of Cell Science 33, 285–99.CrossRefGoogle ScholarPubMed
Dias, E. (1944). Não receptividade do pombo doméstico à infecçã;o por Schizotrypanum. Memórias do Instituto Oswaldo Cruz 40, 191–3.CrossRefGoogle Scholar
Dvorak, J. A. & Schmunis, G. A. (1972). Trypanosoma cruzi: interaction with mouse peritoneal macrophages. Experimental Parasitology 32, 289–300.CrossRefGoogle ScholarPubMed
Ganapati, P. N. (1948). Cultivation of Trypanosoma cruzi in the developing embryo. Nature, London 162, 963.CrossRefGoogle Scholar
Hoff, R. (1975). Killing in vitro of Trypanosoma cruzi by macrophages from mice immunized with T.cruzi or BCG, and absence of cross-immunity on challenge in vivo. Journal of Experimental Medicine 142, 299–311.CrossRefGoogle ScholarPubMed
Kierzenbaum, F., Ivanyi, J. & Budzko, D. B. (1976). Mechanisms of natural resistance to trypanosomal infection. Role of complement in avian resistance to Trypanosoma cruzi infection. Immunology 30, 1–6.Google Scholar
Kipnis, T. L., Calich, V. L. G. & Dias, da Silva W. (1979). Active entry of bloodstream forms of Trypanosoma cruzi into macrophages. Parasitology 78, 89–98.CrossRefGoogle ScholarPubMed
Kress, Y., Bloom, B. R., Wittner, M., Rowen, A. & Tanowitz, H. (1975). Resistance of Trypanosoma cruzi to killing by macrophages. Nature, London 257, 394–6.CrossRefGoogle ScholarPubMed
Kress, Y., Tanowitz, H., Bloom, B. & Wittner, M. (1977). Trypanosoma cruzi: infection of normal and activated mouse macrophages. Experimental Parasitology 41, 385–96.CrossRefGoogle ScholarPubMed
Krettli, A. U. (1978). Efeito de anticorpos e do complemento sobre tripomastigotas sanguíneos de camundongos infectados corn Trypanosoma cruzi. Thesis. Universidade Federal de Minas Gerais, Brasil.Google Scholar
Krettli, A. U. & Brener, Z. (1976). Protective effects of specific antibodies in Trypanosoma cruzi infections. Journal of Immunology 116, 755–60.CrossRefGoogle ScholarPubMed
Melo, R. C. & Brener, Z. (1978). Tissue tropism of different Trypanosoma cruzi strains. Journal of Parasitology 64, 475–82.CrossRefGoogle ScholarPubMed
Meyer, H. & De Souza, W. (1976). Electron microscopic study of Trypanosoma cruzi periplast in tissue cultures. I. Number and arrangement of the peripheral microtubules in the various forms of the parasite's life cycle. Journal of Protozoology 23, 385–90.CrossRefGoogle ScholarPubMed
Meyer, H. & Xavier de Oliveira, M. (1948). Cultivation of Trypanosoma cruzi in tissue culture: a four year study. Parasitology 39, 91–4.CrossRefGoogle Scholar
Milder, R. V., Kloetzel, J. & Deane, M. P. (1973). Observation on the interaction of peritoneal macrophages with Trypanosoma cruzi. I. Initial phase of the relationship with bloodstream and culture forms. Revista do Instituto de Medicina Tropical de São Paulo 15, 386–92.Google Scholar
Milder, R., Kloetzel, J. & Deane, M. P. (1977). Observation on the interaction of peritoneal macrophages with Trypanosoma cruzi. II. Intracellular fate of bloodstream forms. Revista do Instituto de Medicina Tropical de São Paulo 19, 313–22.Google ScholarPubMed
Muniz, J. & de Freitas, G. (1946). Realização ‘in vitro’ do ciclo do ‘S. cruzi’ no vertebrado, em meios de caldo líquido peritoneal. Revista Brasileira de Biologia 6, 467–84.Google Scholar
Nery-Guimarães, F. & Lage, H. A. (1972). A refratariedade das ayes ao Trypanosoma (Schizotrypanum) cruzi. II. Refratariedade das galinhas desde o nascimento; persistência da refratariedade após bursectomia; infecções em ovos embrionados. Memórias do Instituto Oswaldo Cruz 70, 97–107.CrossRefGoogle Scholar
Nogueira, N., Bianco, C. & Cohn, Z. (1975). Studies on the selective lysis and purification of Trypanosoma cruzi. Journal of Experimental Medicine 142, 224–9.CrossRefGoogle ScholarPubMed
Nogueira, N. & Cohn, Z. (1976). Trypanosoma cruzi: mechanism of entry and intracellular fate in mammalian cells. Journal of Experimental Medicine 143, 1402–20.CrossRefGoogle ScholarPubMed
Romaña, C. & Meyer, H. (1942). Estudo do ciclo evolutivo do Schizotrypanum cruzi em cultura de tecidos de embrião de galinha. Memórias do Instituto Oswaldo Cruz 37, 19–27.CrossRefGoogle Scholar
Silva, L. H. P. & Nussenzweig, V. (1953). Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco. Polio Clinica Biologica 20, 191–207.Google Scholar
Tanowitz, H., Wittner, M., Kress, Y. & Bloom, B. (1975). Studies of in vitro infection by Trypanosoma cruzi. I. Ultrastructural studies on the invasion of macrophages and L-cells. American Journal of Tropical Medicine and Hygiene 24, 25–33.CrossRefGoogle ScholarPubMed
Warren, L. (1960). Metabolism of Schizotrypanum cruzi Chagas. I. Effect of cultures age and substrate concentration on respiratory rate. Journal of Parasitology 46, 529–39.CrossRefGoogle Scholar
Williams, D. M. & Remington, J. S. (1977). Effect of human monocytes and macrophages on Trypanosoma cruzi. Immunology 32, 19–23.Google ScholarPubMed