Hostname: page-component-745bb68f8f-5r2nc Total loading time: 0 Render date: 2025-01-27T17:13:00.113Z Has data issue: false hasContentIssue false
  • English
  • Français

The interaction in vitro between bovine immunoglobulin and juvenile Fasciola hepatica

Published online by Cambridge University Press:  06 April 2009

W. P. H. Duffus  and
D. Franks
W. P. H. Duffus
Affiliation:
University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP
D. Franks
Affiliation:
University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP
Get access Rights & Permissions [Opens in a new window]

Summary

Freshly excysted Fasciola hepatica possess an outer glycocalyx which stimulates an antibody response in cattle infected with F. hepatica metacercariae. Sera from animals receiving either a single or a double infection were examined for levels of IgM, IgG1 and IgG2 specific for the outer glycocalyx of intact live juvenile F. hepatica. High levels of specific IgG1 were found to predominate. Using positive sera in an indirect fluorescent antibody assay, the bovine immunoglobulins labelled the outer glycocalyx in a characteristic reticulated pattern. On incubation at 37 °C this pattern rapidly broke up into discrete clumps and progressed until the entire antibody–outer glycocalyx complex was shed. The shedding phenomenon was shown to be continuous and, by using preparations of Fab fragments, was not due to the bivalent binding of the sensitizing antibody.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 1 , February 1981 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anwar, A. R. E., Smithers, S. R. & Kay, A. B. (1979). Killing of schistosomula of Schistosoma mansoni coated with antibody and/or complement by human leucocytes in vitro; requirement for complement in preferential killing by eosinophils. Journal of Immunology 122, 628–37.CrossRefGoogle ScholarPubMed
Armour, J. & Dargie, J. D. (1974). Immunity to Fasciola hepatica in the rat. Successful transfer of immunity by lymphoid cells and by serum. Experimental Parasitology 35, 381–8.CrossRefGoogle ScholarPubMed
Bennett, C. E. (1978). The identification of soluble adult antigen on the tegumental surface of juvenile Fasciola hepatica. Parasitology 77, 325–32.CrossRefGoogle ScholarPubMed
Bloom, B. R. (1979). Games parasites play: how parasites evade immune surveillance. Nature, London 279, 21–6.CrossRefGoogle ScholarPubMed
Brink, L. H., McLaren, D. J. & Smithers, S.R. (1977). Schistosoma mansoni: a comparative study of artificially transformed schistosomula and schistosomula recovered after cercarial penetration of isolated skin. Parasitology 74, 73–86.CrossRefGoogle ScholarPubMed
Butler, J.E., Winter, A. J. & Wagner, G. G. (1971). Bovine immune system. Journal of Dairy Science 54, 1309–40.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Sturrock, R. F., Houba, V., Mahmoud, A. A. F., Sher, A. & Rees, C. H. (1975). Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature, London 256, 727–9.CrossRefGoogle ScholarPubMed
Doyle, J.J. (1972). Evidence of an acquired resistance in calves to a single experimental infection of Fasciola hepatica. Research in Veterinary Science 13, 456–9.CrossRefGoogle ScholarPubMed
Duffus, W. P. H., Butterworth, A. E., Wagner, G. G., Preston, J. M. & Franks, D. (1978). Antibody-dependent cell-mediated cytotoxicity in cattle: activity against 51Cr-labelled chicken erythrocytes coated with protozoal antigens. Infection and Immunity 22, 492–501.CrossRefGoogle Scholar
Duffus, W. P. H. & Wagner, G. G. (1974). The specific immunoglobulin response in cattle immunized with Theileria parva (Muguga) stabilate. Parasitology 69, 31–41.CrossRefGoogle ScholarPubMed
Duffus, W. P. H., Wagner, G. G. & Preston, J. M. (1978). Initial studies on the properties of a bovine lymphoid cell culture infected with Theileria parva. Clinical and Experimental Immunology 34, 347–53.Google ScholarPubMed
Goldring, O. L., Clegg, J. A., Smithers, S. R. & Terry, R. J. (1976). Acquisition of human blood group antigens by Schistosoma mansoni. Clinical and Experimental Immunology 26, 181–7.Google ScholarPubMed
Hanna, R. E. B. (1978). A possible immunological role for the tegument of Fasciola hepatica. Parasitology 77, ix.Google Scholar
Hanna, R. E. B. & Jura, W. (1977). Antibody response of calves to a single infection of Fasciola gigantica determined by an indirect fluorescent antibody technique. Research in Veterinary Science 22, 339–42.CrossRefGoogle ScholarPubMed
El Harith, A. (1977). Observations on the influence of the intermediate host in experimental fascioliasis. Ph.D. thesis, Utrecht University, Holland.Google Scholar
Hockley, D. J. & McLaren, D. J. (1973). Schistosomá mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 13–25.CrossRefGoogle ScholarPubMed
Hughes, D. L., Anderson, J. C. & Harness, E. (1976). Fasciola hepatica: the effect of challenge by the subcutaneous or intraperitoneal route with living adult flukes in sensitized rats. Experimental Parasitology 40, 355–62.CrossRefGoogle ScholarPubMed
Hughes, D. L. & Harness, E. (1973). Attempts to demonstrate a ‘Host antigen’ effect by the experimental transfer of adult Fasciola hepatica into recipient animals immunized against the donor. Research in Veterinary Science 14, 151–4.CrossRefGoogle ScholarPubMed
Hughes, D. L., Harness, E. & Doy, T. G. (1977). Loss of ability to kill Fasciola hepatica in sensitized rats. Nature, London 267, 517–18.CrossRefGoogle Scholar
Kendall, S. B., Sinclair, I. S., Everett, G. & Parfitt, J. W. (1978). Resistance to Fasciola hepatica in cattle. I. Parasitological and serological observations. Journal of Comparative Pathology 88, 115–22.CrossRefGoogle ScholarPubMed
McLaren, D. J., Mackenzie, C. D. & Ramalho-Pinto, F. J. (1977). Ultrastructural observations in the in vitro interaction between rat eosinophils and some parasite helminths (Schistosoma mansoni, Trichinella spiralis and Nippostrongylus brasiliensis). Clinical and Experimental Immunology 30, 105–18.Google ScholarPubMed
Meek, A. H. & Morris, R. S. (1979). The effect of prior infection with Fasciola hepatica On the resistance of sheep to the same parasite. Australian Veterinary Journal 55, 61–4.CrossRefGoogle Scholar
Movsesijan, M., Javanovic, B., Aalund, O. & Nansen, P. (1975). Immune response of sheep to Fasciola infection. Research in Veterinary Science 18, 171–4.CrossRefGoogle Scholar
Murphy, F. A., Osebold, J. W. & Aalund, O. (1966). Kinetics of the antibody response to Anaplasma marginale infection. Journal of Infectious Diseases 116, 99–111.CrossRefGoogle ScholarPubMed
Patterson, J. M., Deyoe, B. L. & Stone, S. S. (1976). Identification of immunoglobulins associated with complement fixation, agglutination, and low pH buffered antigen tests for brucellosis. American Journal of Veterinary Research 37, 319–24.Google Scholar
Pearson, C. W. & Lloyd, L. C. (1972). Immunoglobulins of cattle affected by contagious bovine pleuropneumonia. Research in Veterinary Science 13, 230–5.CrossRefGoogle ScholarPubMed