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The ingestion of particulate lipid containing a fluorochome dye, Acridine Orange, by Trypanosoma lewisi

Published online by Cambridge University Press:  06 April 2009

R. M. Wotton  and
D. A. Becker
R. M. Wotton
Affiliation:
Department of Anatomy, School of Medicine, Louisiana State University, New Orleans, Department of Zoology, University of Arkansas, Fayetteville
D. A. Becker
Affiliation:
Department of Anatomy, School of Medicine, Louisiana State University, New Orleans, Department of Zoology, University of Arkansas, Fayetteville
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Extract

When Acridine Orange was dispersed in cod-liver oil, its characteristic green fluorescence remained unaltered following absorption of this mixture into the peripheral circulation from the intestine. This result implies that there was little or no change in the lipid or its suspensoid as a result of undergoing this physiological process. The selective combination of this dye in aqueous solution with the cytoplasmic components of specimens of Trypanosoma lewisi, also present in the blood smears with the droplets of the green-appearing oil, resulted in an alteration of fluorescence as indicated by the rose red appearance of these organisms.

The present findings on the absorption of lipid droplets, containing a fluorochrome, by trypanosomes occurring simultaneously in the circulation of rats confirm similar observations reported by Wotton & Halsey (1957). The possibility is suggested again that fats and oils may serve as pharmacological vehicles for orally administered trypanosomides for the elimination of trypanosomes.

Part of this study was carried out in the department of Zoology and Anatomy of the University of Nebraska, Lincoln, Nebraska, and was supported in part by a grant from the Nebraska Heart Association for 1959. We would like also to extend our appreciation to the Division of Laboratories of the Nebraska State Health Department of Lincoln, Nebraska, for the use of their ‘Fluorex’, ultraviolet illumination microscope unit.

Type
Research Article
Information
Parasitology , Volume 53 , Issue 1-2 , May 1963 , pp. 163 - 167
Copyright
Copyright © Cambridge University Press 1963

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References

REFERENCES

Bennett, H. S. (1956). The concepts of membrane flow and membrane vesiculation as mechanisms for active transport and ion pumping. J. Biophys. Biochem. Cytol. 2 (no. 4, pt. 2), 99–1–3 (Suppl.).CrossRefGoogle ScholarPubMed
Cain, A. J. (1950). The histochemistry of lipoids in animals. Biol. Rev. 25, 73–112.CrossRefGoogle ScholarPubMed
De Ment, J. and Drake, H. C. (1942). Fluorescent Chemicals and their Application. Brooklyn: Chemical Publishing Co.Google Scholar
De Ment, J. (1945). Fluorochemistry, New York: Chemical Publishing Co.Google Scholar
Deuel, H. J. Jr (1951). Lipids, Vol. 1, p. 668. New York: Interscience Publishers.Google Scholar
Haitinger, M. (1934). Die Methoden der Fluoreszenzmikroskopie. In: Abderhalden, E. (ed.) Handbuch der biologischen Arbeitsmethoden, Abt. II. Physikalische Methoden. Teil 3, Hälfte 1, pp. 3307–37. Wien: Urban & Schwarzenberg.Google Scholar
Haitinger, M. (1937). Die Fluorescenzanalyse in der Mikrochemie. Monographien aus dem Gesamtgebiete der Mikrochemie, pp. 46, 49, 159, 192. Wien and Leipzig: Emil Haim and Co.Google Scholar
Haitinger, M. & Hamperl, H. (1933). Die Anwendung des Fluorescenzmikroskops zur Untersuchung tierischer Gewebe. Z. mikroskop.-anat. Forsch. 33, 193–221.Google Scholar
Hewitt, W. (1954). A histochemical study of fat absorption in the small intestine of the rat. Quart. J. micr. Sci. 95, 153–7.Google Scholar
Hewett, W. (1956). Further observations on the histochemistry of fat absorption in the small intestine of the rat. Quart. J. micr. Sci. 97, 199–203.Google Scholar
Hewett, W. (1958). The absorption of oleic acid from the small intestine of the rat. Quart. J. micr. Sci. 99, 25–7.Google Scholar
Popper, H. (1944). Distribution of vitamin A in tissue as visualized by fluorescence microscopy. Physiol. Rev. 24, 205–24.CrossRefGoogle Scholar
Wotton, R. M. & Halsey, H. R. (1957). The ingestion of particulate fat from the blood by Trypanosoma lewisi and Trypanosoma equiperdum. Parasitology, 47, 427–31.CrossRefGoogle ScholarPubMed
Wotton, R. M. & Zwemer, R. L. (1935). A note on ‘Glychrogel’ mounting solution. Stain Tech. 10, 21–22.CrossRefGoogle Scholar