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The influence of Blastocrithidia triatomae (Trypanosomatidae) on the reduviid bug Triatoma infestans: in vivo and in vitro diuresis and production of diuretic hormone

Published online by Cambridge University Press:  06 April 2009

Antonia Schnitker ,
G. A. Schaub  and
S. H. P. Maddrell
Antonia Schnitker
Affiliation:
AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, England
G. A. Schaub
Affiliation:
AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, England
S. H. P. Maddrell
Affiliation:
AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, England
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Summary

The homoxenous flagellate Blastocrithidia triatomae disturbs excretion in Triatoma infestans. During the first 4 and 24 h after feeding, infected 5th instars excreted approximately a 2·5-fold smaller volume of urine. Consequently, about double the normal volume of haemolymph could be obtained from these infected bugs 24 h after feeding. Surprisingly, the in vitro secretion rates of Malpighian tubules from uninfected and infected bugs were nearly identical. In addition, the storage and release of diuretic hormone in infected bugs was sufficient to induce normal secretion rates by isolated Malpighian tubules. Therefore, the previously observed ultrastructural alterations in the upper Malpighian tubules of infected bugs do not result in a disturbed in vitro secretion, although in vivo excretion is reduced.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 9 - 17
Copyright
Copyright © Cambridge University Press 1988

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