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The indicator system of the Sabin-Feldman phenomenon in Toxoplasma

Published online by Cambridge University Press:  06 April 2009

C. Kulasiri
C. Kulasiri
Affiliation:
Department of Parasitology, London School of Hygiene and Tropical Medicine, London
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Extract

Azure A, azure B and Nile blue in both neutral and alkaline solutions were found to be suitable for use as indicator dyes in the Sabin-Feldman test. Toluidine blue, brilliant cresyl blue and thionine were also suitable as neutral solutions. Azure C, phloxine, phloxine B, neutral red, methyl green, pyronine Y, eosin and celestine blue were found to be unsuitable. Azure B and toluidine blue in saline solutions were serviceable up to 5 months after preparation. The significance of these findings is discussed.

Type
Research Article
Information
Parasitology , Volume 50 , Issue 1-2 , May 1960 , pp. 13 - 20
Copyright
Copyright © Cambridge University Press 1960

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References

REFERENCES

Beverley, J. K. A. & Beattie, C. P. (1952). Standardization of the dye test for toxoplasmosis. J. Clin. Path. 5, 350–3.CrossRefGoogle ScholarPubMed
Braunsteiner, H., Pakesch, F. & Thalhammer, O. (1957). Elektronenmikroskopische Untersuchungen über die Morphologie des Toxoplasma gondii und das Wesen des Farbtestes nach Sabin-Feldman. Wien. Z. inn. Med. 38, 16–27.Google Scholar
Bringmann, G. & Holz, J. (1953). Licht- und elektronenmikroskopische Untersuchungen zum Sero-Farbtest auf Toxoplasmose nach Sabin und Feldman. Z. Hyg. InfektKr. 138, 151–4.CrossRefGoogle ScholarPubMed
Cathie, I. A. B. & Dudgeon, J. A. (1949). The laboratory diagnosis of toxoplasmosis. J. Clin. Path. 2, 259–66.CrossRefGoogle ScholarPubMed
Conn, H. J. (1953). Biological Stains. A Hand Book on the Nature and Uses of the Dyes Employed in the Biological Laboratory, 6th ed. Geneva, N.Y.: W. F. Humphrey Press Inc.Google Scholar
Fehrle, G. (1955). Untersuchungen über das Wesen des Sero-Farbtestes nach Sabin und Feldman. Inaugural-Dissertation zur Erlangung des Doktor-Grades bei der Veterinär-Medizinschen Fakultät der Justus Liebig-Hochschule zu Giessen.Google Scholar
Frenkel, J. K. & Jacobs, L. (1958). Ocular toxoplasmosis. Arch. Ophthal., N.Y., 59, 260–79.CrossRefGoogle ScholarPubMed
Grönroos, P. (1955). Studies on Toxoplasma and the serology of toxoplasmosis. Ann. Med. exp. Fenn. 33, suppl. 11, 1–113.Google Scholar
Holmes, W. C. (1927). Subsidiary dyes in methylene blue. Stain Tech. 2, 71–3.Google Scholar
Holmes, W. C. (1928). The spectrophotometric evaluation of mixtures of methylene blue and trimethyl thionin. Stain Tech. 3, 45–8.CrossRefGoogle Scholar
Jacobs, L. & Cook, M. K. (1954). Variations in the dye test for toxoplasmosis. Amer. J. Trop. Med. Hyg. 3, 860–7.Google Scholar
Kulasiri, C. & Dasgupta, B. (1959). A cytochemical investigation of the Sabin-Feldman phenomenon in Toxoplasma gondii and an explanation of its mechanism on this basis. Parasitology (in the Press).CrossRefGoogle Scholar
Manning, J. D. & Reid, J. D. (1956). Toxoplasmosis in New Zealand. A serological survey. N.Z. Med. J. 55, 441–8.Google ScholarPubMed
Sabin, A. B. & Feldman, H. A. (1948). Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoan parasite (Toxoplasma). Science, 108, 660–3.CrossRefGoogle Scholar
Westphal, A. (1951). Abhängigkeit der Titerhöhe des Sabin-Feldman-Testes vom Methylenblau. Z. Tropenmed. Parasit. 3, 72–6.Google Scholar
Westphal, A., Bimmer, E. & Palm, G. (1957). Über die Wirkung verschiedener Ionen auf die Titerhöhe des Toxoplasma-Serofarbtestes. Z. Tropenmed. Parasit. 8, 520–31.Google Scholar