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In vitro polarization of carp leucocytes in response to the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae)

Published online by Cambridge University Press:  06 April 2009

D. T. Richards ,
D. Hoole ,
J. W. Lewis ,
E. Ewens  and
C. Arme
D. T. Richards*
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, Keele University, Keele, Staffs ST5 5BG
D. Hoole
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, Keele University, Keele, Staffs ST5 5BG
J. W. Lewis
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX
E. Ewens
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 OEX
C. Arme
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, Keele University, Keele, Staffs ST5 5BG
*
* Corresponding author.
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Sumary

An in vitro assay was used to determine the effects of Sanguinicola inermis adults and cercariae on the polarization responses of pronephric leucocytes of carp. Leucocytes were isolated and exposed to live adult flukes or cercariae for up to 48 h. Differences in polarization responses were related to the presence of the parasite, the presence or absence of carp serum and the time of incubation. The mean proportions of cells exhibiting polarization in unstimulated controls ranged from 5 to 30% over the experimental period. Within 15 min of exposure to adults or cercariae, significant increases in mean polarization responses were observed of up to 75% and levels remained higher than control values for over 24 h. Overall, the presence of normal carp serum, either untreated or heat inactivated, did not enhance the polarization responses of leucocytes incubated with only cercariae or adults. However, between 0·25 and 3 h, the presence of carp serum with cercariae significantly enhanced polarization responses when compared with cells incubated with cercariae alone.

Keywords

carpCyprinus capioSanguinicola inermispolarizationluecocyte
Type
Research Article
Information
Parasitology , Volume 112 , Issue 5 , May 1996 , pp. 509 - 513
Copyright
Copyright © Cambridge University Press 1996

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References

REFERENCES

Butterworth, A. C. (1984). Cell mediated damage to helminths. Advances in Parasitology 23, 143–235.CrossRefGoogle ScholarPubMed
Cenini, P. (1984). The ultrastructure of leucocytes in carp (Cyprinus carpio). Journal of Zoology 204, 509–20.CrossRefGoogle Scholar
Griffin, B. R. (1984). Random and directed migration of trout (Salmo gairdneri) leukocytes: activation by antibody, complement and normal serum components. Developmental and Comparative Immunology 8, 589–97.CrossRefGoogle ScholarPubMed
Hoole, D. & Arme, C. (1982). Ultrastructural studies on the cellular response of roach, Rutilus rutilus L., to the plerocercoid larva of the pseudophyllidean cestode, Ligula intestinalis. Journal of Fish Diseases 5, 131–44.CrossRefGoogle Scholar
Hoole, D. & Arme, C. (1930 a). Ligula intestinalis (Cestoda: Pseudophyllidea): an ultrastructural study on the cellular response of roach fry, Rutilus rutilus. International Journal for Parasitology 13, 359–63.CrossRefGoogle Scholar
Hoole, D. & Arme, C. (1936 b). Ultrastructural studies on the cellular response of fish hosts following experimental infection with the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea). Parasitology 87, 139–49.CrossRefGoogle Scholar
Hunt, T. C. & Rowley, A. F. (1986). Leukotriene B4 induces enhanced migration of fish leucocytes in vitro. Immunology 59, 563–8.Google ScholarPubMed
Kirk, R. S. & Lewis, J. W. (1992). The laboratory maintenance of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae). Parasitology 104, 121–7.CrossRefGoogle ScholarPubMed
Kirk, R. S. & Lewis, J. W. (1993). The life-cycle and morphology of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae). Systematic Parasitology 25, 125–33.CrossRefGoogle Scholar
Lee, R. S. (1990). The development of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae) in the common carp Cyprinus carpio L. Ph.D. thesis, Royal Holloway and Bedford New College, University of London.Google Scholar
MacArthur, J. I., Thomson, A. W. & Fletcher, T. C. (1985). Aspects of leucocyte migration in the plaice, Pleuronectes platessa L. Journal of Fish Biology 27, 667–76.CrossRefGoogle Scholar
McLaren, D. J. (1980). Schistosoma mansoni: The Parasite Surface in Relation to Host Immunity. Chichester: Research Studies Press.Google Scholar
Nash, K. A., Fletcher, T. C. & Thomson, A. W. (1986). Migration of fish leucocytes in vitro: the effect of factors which may be involved in mediating inflammation. Veterinary Immunology and Immunopathology 12, 83–92.CrossRefGoogle ScholarPubMed
Obenauf, S. D. & Smith, S. H. (1985). Chemotaxis of nurse shark leukocytes. Developmental and Comparative Immunology 9, 221–30.CrossRefGoogle ScholarPubMed
Richards, D. T., Hoole, D., Lewis, J. W., Ewens, E. & Arme, C. (1994 a). Ultrastructural observations on the cellular response of carp (Cyprinus carpio L.) to eggs of the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae). Journal of Fish Diseases 17, 439–46.CrossRefGoogle Scholar
Richards, D. T., Hoole, D., Lewis, J. W., Ewens, E. & Arme, C. (1946). Changes in the cellular composition of the spleen and pronephros of carp Cyprinus carpio infected with the blood fluke Sanguinicola inermis (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms 19, 173–9.CrossRefGoogle Scholar
Richards, D. T., Hoole, D., Lewis, J. W., Ewens, E. & Arme, C. (1996). Stimulation of carp Cyprinus carpio lymphocytes by the blood fluke Sanguinicola inermis (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms.CrossRefGoogle Scholar
Sharp, G. J. E., Pike, A. W. & Secombes, C. J. (1991). Leucocyte migration in rainbow trout (Oncorhynchus mykiss [Walbaum]): optimization of migration conditions and responses to host and pathogen (Diphyllobothrium dendriticum [Nitzsch]) derived chemoattractants. Developmental and Comparative Immunology 15, 295–305.CrossRefGoogle ScholarPubMed
Sher, A. & Coffman, R. L. (1992). Regulation of immunity to parasites by T cells and B cell-derived cytokines. Annual Review of Immunology 10, 385–409.CrossRefGoogle Scholar
Shields, J. M. & Haston, W. S. (1985). Behaviour of neutrophil leucocytes in uniform concentrations of chemotactic factors: contraction waves, cell polarity and persistence. Journal of Cell Science 74, 75–93.CrossRefGoogle ScholarPubMed
Sommerville, C. & Iqbal, N. A. M. (1991). The process of infection, migration, growth and development of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae) in carp, Cyprinus carpio L. Journal of Fish Diseases 14, 211–19.CrossRefGoogle Scholar
Suzuki, Y. & Iida, T. (1992). Fish granulocytes in the process of inflammation. Annual Review of Fish Diseases 2, 149–60.CrossRefGoogle Scholar
Taylor, M. J. & Hoole, D. (1993). Ligula intestinalis (L.) (Cestoda: Pseudophyllidea): polarization of cyprinid leucocytes as an indicator of host- and parasite-derived chemoattractants. Parasitology 107, 433–40.CrossRefGoogle ScholarPubMed
Wilkinson, P. C. & Haston, W. S. (1988). Chemotaxis: an overview. In Immunological Techniques. Part L, Chemotaxis and Inflammation. Methods in Enzymology, Vol. 162 (ed. Di Sabato, G.), pp. 3–16. London: Academic Press.Google Scholar
Woo, P. T. K. (1992). Immunological responses of fish to parasitic organisms. Annual Review of Fish Diseases 2, 339–66.CrossRefGoogle Scholar
Zigmond, S. M., Levitsky, H. L. & Kreel, B. J. (1981). Cell polarity: an examination of its behavioural expression and its consequences for polymorphonuclear leukocyte chemotaxis. Journal of Cell Biology 89, 585–92.CrossRefGoogle ScholarPubMed