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In vitro isolation from Amblyomma ovale (Acari: Ixodidae) and ecological aspects of the Atlantic rainforest Rickettsia, the causative agent of a novel spotted fever rickettsiosis in Brazil

Published online by Cambridge University Press:  30 January 2013

M. P. J. SZABÓ ,
F. A. NIERI-BASTOS ,
M. G. SPOLIDORIO ,
T. F. MARTINS ,
A. M. BARBIERI  and
M. B. LABRUNA
M. P. J. SZABÓ*
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87 Cidade Universitária, CEP: 05508-270, São Paulo, SP Brasil Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Uberlândia Av. Pará, 1720/Campus Umuarama-Bloco 2T, Uberlândia, Minas Gerais, Brasil
F. A. NIERI-BASTOS
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87 Cidade Universitária, CEP: 05508-270, São Paulo, SP Brasil
M. G. SPOLIDORIO
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87 Cidade Universitária, CEP: 05508-270, São Paulo, SP Brasil
T. F. MARTINS
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87 Cidade Universitária, CEP: 05508-270, São Paulo, SP Brasil
A. M. BARBIERI
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87 Cidade Universitária, CEP: 05508-270, São Paulo, SP Brasil
M. B. LABRUNA
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87 Cidade Universitária, CEP: 05508-270, São Paulo, SP Brasil
*
*Corresponding author: Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Uberlândia Av. Pará, 1720/Campus Umuarama-Bloco 2T, Uberlândia, Minas Gerais, Brasil. Tel: +55 34 3218 2691. Fax: +55 34 3218 2521. E-mail: [email protected]
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Summary

Recently, a novel human rickettsiosis, namely Atlantic rainforest spotted fever, was described in Brazil. We herein report results of a survey led around the index case in an Atlantic rainforest reserve in Peruibe municipality, southeastern Brazil. A Rickettsia parkeri-like agent (Rickettsia sp. Atlantic rainforest genotype) and Ricketsia bellii were isolated from adult Amblyomma ovale ticks collected from dogs. Molecular evidence of infection with strain Atlantic rainforest was obtained for 30 (12·9%) of 232 A. ovale adult ticks collected from dogs. As many as 88·6% of the 35 examined dogs had anti-Rickettsia antibodies, with endpoint titres at their highest to R. parkeri. High correlation among antibody titres in dogs, A. ovale infestations, and access to rainforest was observed. Amblyomma ovale subadults were found predominantly on a rodent species (Euryoryzomys russatus). From 17 E. russatus tested, 6 (35·3%) displayed anti-Rickettsia antibodies, with endpoint titres highest to R. parkeri. It is concluded that Atlantic rainforest genotype circulates in this Atlantic rainforest area at relatively high levels. Dogs get infected when bitten by A. ovale ticks in the forest, and carry infected ticks to households. The role of E. russatus as an amplifier host of Rickettsia to A. ovale ticks deserves investigation.

Keywords

Atlantic rainforest spotted feverAmblyomma ovaleBrazilisolationecology
Type
Research Article
Information
Parasitology , Volume 140 , Issue 6 , May 2013 , pp. 719 - 728
Copyright
Copyright © Cambridge University Press 2013

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