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In vitro and in vivo efficacy of diamidines against Trypanosoma equiperdum strains

Published online by Cambridge University Press:  23 November 2017

Kirsten Gillingwater
Kirsten Gillingwater*
Affiliation:
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstrasse 57, Basel 4002, Switzerland University of Basel, Petersplatz 1, Basel 4001, Switzerland
*
Author for correspondence: Kirsten Gillingwater, E-mail: [email protected]
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Abstract

Trypanosoma equiperdum is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal to animal via coitus. At present, there is no officially recognized form of chemotherapeutic treatment and therefore all confirmed (or suspected) cases of infected animals must be slaughtered immediately. For many global communities and farming populations, which rely heavily on their animals for their livelihood, such stringent regulations can seriously enhance the socio-economic problems attributing to poverty. Two reference drugs, together with 37 novel diamidine compounds were tested in vitro using a 72 h drug sensitivity assay to determine their efficacy against two axenically adapted T. equiperdum strains. Further in vivo investigations in mouse models of infection against 4 ‘true’ T. equiperdum strains were performed using the 17 most active diamidines. Single bolus doses of 10 mg kg−1, given i.p. were administered to NMRI mice infected with one of the 4 T. equiperdum strains. The results obtained from this study show that experimentally T. equiperdum can indeed be effectively treated with chemotherapy using in vivo mouse models of infection.

Keywords

chemotherapydiamidinesdourinein vitro drug sensitivityin vivo efficacyTrypanosoma equiperdum
Type
Research Article
Information
Parasitology , Volume 145 , Issue 7 , June 2018 , pp. 953 - 960
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Copyright
Copyright © Cambridge University Press 2017 

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