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Impairment of growth of Leishmania donovani by Trypanosoma brucei during co-culture

Published online by Cambridge University Press:  06 April 2009

I. Coppens ,
B. H. Ter Kuile  and
F. R. Opperdoes
I. Coppens
Affiliation:
Cell Biology Unit, University of Louvain Medical School and International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium
B. H. Ter Kuile
Affiliation:
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium
F. R. Opperdoes
Affiliation:
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, 75 avenue Hippocrate, 1200–Brussels, Belgium
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Summary

Cells of Leishmania donovani in co-culture with Trypanosoma brucei, were severely affected in their growth, resulting in swelling and subsequent lysis. Similar effects were also observed when Crithidia luciliae or Phytomonas sp. were co-cultured with T. brucei. Direct contact between the cells under investigation and T. brucei was necessary because T. brucei did not hamper the growth of the other trypanosomatids, when separated by a filter with 0.2 μm pore size. Examination of this phenomenon at the ultrastructural level, in a co-culture of L. donovani and T. brucei, suggests that the plasma membrane permeability is increased in the former, as a result of a close cellular contact between the two cell types.

Keywords

Trypanosoma bruceiLeishmania donovani infantumPhytomonasCrithidia luciliaecytolysisinteractions
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 393 - 398
Copyright
Copyright © Cambridge University Press 1992

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