Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-26T22:06:17.249Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunohistochemical and ultrastructural localization of Schistosoma mansoni soluble egg antigens processed by the infected host

Published online by Cambridge University Press:  06 April 2009

J. J. P. M. Bogers ,
H. A. M. Nibbeling ,
A. M. Deelder  and
E. A. E. Van Marck
J. J. P. M. Bogers
Affiliation:
Laboratory of Pathology, University of Antwerp (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium
H. A. M. Nibbeling
Affiliation:
Laboratory of Parasitology, University of Leiden, The Netherlands
A. M. Deelder
Affiliation:
Laboratory of Parasitology, University of Leiden, The Netherlands
E. A. E. Van Marck*
Affiliation:
Laboratory of Pathology, University of Antwerp (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium
*
* Corresponding author. Tel: + 32 3820 2534. Fax: + 32 3820 2532. E-mail: [email protected].
Get access Rights & Permissions [Opens in a new window]

Summary

The detection of egg-derived antigens in the serum and urine of Schistosoma mansoni-infected individuals and experimental animals would provide an alternative method to assess the tissue egg burden. The detected levels are, however, not only a function of the amounts of antigen produced, but also of the processing or clearance by the host. In the present study the immunolocalization pattern of antigens using 2 recently described monoclonal antibodies to repetitive carbohydrate epitopes of S. mansoni soluble egg antigen (114–5B1–A and 114–4D12–A) in various organs of the host was investigated. In the liver strong immunoreactivity could be detected around the entrapped eggs and in egg-shells, as well as in Kupffer cells accumulating both antigen and schistosomal pigment. In the spleen, immunohistochemistry revealed antigen in the plasma as well as in secondary lysosomes of macrophages. Strong labelling was found in the vesicles of the eosinophilic granulocytes: indirect evidence perhaps for the presence of antigen–antibody complexes. In conclusion, the secreted egg antigens were sequestered in the reticulo-endothelial macrophages of the liver and the spleen as already partly described for worm-derived antigens. The presence of large quantities of antigenic material in the spleen could suggest an important role of this organ in the clearance of antigen and might even provide an additional explanation for the hepatosplenomegaly mainly present in S. mansoni-infected children.

Keywords

Schistosoma mansonicirculating antigensimmunohistochemistryimmunogoldultrastructurealkaline phosphatase
Type
Research Article
Information
Parasitology , Volume 112 , Issue 6 , June 1996 , pp. 537 - 543
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Berggren, W. L. & Weller, T. H. (1967). Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 16, 606–12.CrossRefGoogle ScholarPubMed
Bloom, W. & Fawcett, D. W. (1975). A Textbook of Histology, 10th Edn.Philadelphia: W. B. Saunders Company.Google Scholar
Bogers, J. J., Nibbeling, H. A., Deelder, A. M. & Van Marck, E. A. (1995 a). Quantitative and morphological aspects of Unicryl versus Lowicryl K4M embedding in immunoelectron microscopical studies. Journal of Histochemistry and Cytochemistry, (in Press)Google Scholar
Bogers, J. J. P. M., Nibbeling, H. A. M., Kumar, Singh S., Van Marck, E. A. E. & Deelder, A. M. (1995 b). Ultrastructural localization of two circulating egg antigens in miracidia of Schistosoma mansoni. Parasitology 110, 365–70.CrossRefGoogle ScholarPubMed
Bogers, J. J. P. M., Nibbeling, H. A. M., Van Marck, E. A. E. & Deelder, A. M. (1995 c). Immunoelectronmicroscopical localization of a circulating antigen in the excretory system of Schistosoma mansoni. Parasitology Research 81, 375–81.CrossRefGoogle Scholar
Bogers, J. J. P. M., Nibbeling, H. A. M., Van Marck, E. A. E. & Deelder, A. M. (1994). Immunofluorescent visualization of the excretory and gut system of Schistosoma mansoni by confocal laser scanning microscopy. American Journal of Tropical Medicine and Hygiene 50, 612–19.CrossRefGoogle ScholarPubMed
Carlier, Y., Bout, D., Strecker, G., Debray, H. & Capron, A. (1980). Purification, immunochemical, and biologic characterization of the Schistosoma circulating M antigen. Journal of Immunology 124, 2442–50.CrossRefGoogle ScholarPubMed
Deelder, A. M., De Jonge, N., Boerman, O. C., Fillié, Y. E., Hilberath, G. W., Rotmans, J. P., Gerritse, M. J. & Schut, D. W. O. A. (1989). Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 40, 268–72.CrossRefGoogle ScholarPubMed
Deelder, A. M., Klappe, H. T., Van Den Aardweg, G. J. & Van Meerbeke, E. H. (1976). Schistosoma mansoni: demonstration of two circulating antigens in infected hamsters. Experimental Parasitology 40, 189–97.CrossRefGoogle ScholarPubMed
Deelder, A. M., Kornelis, D., Van Marck, E. A., Eveleigh, P. C. & Van Egmond, J. G. (1980). Schistosoma mansoni: characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse, hamster, and human infections. Experimental Parasitology 50, 16–32.CrossRefGoogle ScholarPubMed
De Jonge, N., Gryseels, B., Hilberath, G. W., Polderman, A. M. & Deelder, A. M. (1988). Detection of circulating anodic antigen by ELISA for seroepidemiology of schistosomiasis mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 591–4.CrossRefGoogle ScholarPubMed
De Jonge, N., Rabello, A. L., Krijger, F. W., Kremsner, P. G., Rocha, R. S., Katz, N. & Deelder, A. M. (1991). Levels of the schistosome circulating anodic and cathodic antigens in serum of schistosomiasis patients from Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 756–9.CrossRefGoogle ScholarPubMed
De Vlas, S. J. & Gryseels, B. (1992). Underestimation of Schistosoma mansoni prevalences. Parasitology Today 8, 274–7.CrossRefGoogle ScholarPubMed
De Water, R., Fransen, J. A. & Deelder, A. M. (1986 a). Ultrastructural localization of the circulating anodic antigen in the digestive tract of Schistosoma mansoni using monoclonal antibodies in an immunogold labeling procedure. American Journal of Tropical Medicine and Hygiene 35, 549–58.CrossRefGoogle Scholar
De Water, R., Fransen, J. A. & Deelder, A. M. (1986 b). Ultrastructural localization of the circulating cathodic antigen in the digestive tract of various life-cycle stages of Schistosoma mansoni. Zeitschrift für Parasitenkunde 72, 635–46.CrossRefGoogle ScholarPubMed
De Water, R., Van Marck, E. A., Fransen, J. A. & Deelder, A. M. (1987). Ultrastructural localization of the circulating anodic antigen and the circulating cathodic antigen in the liver of mice infected with Schistosoma mansoni: a sequential study. Experimental Parasitology 64, 499–509.CrossRefGoogle ScholarPubMed
El-Dosoky, I., Van Marck, E. A. & Deelder, A. M. (1984). Presence of Schistosoma mansoni antigens in liver, spleen and kidney of infected mice: a sequential study. Zeitschrift für Parasitenkunde 70, 491–7.CrossRefGoogle ScholarPubMed
Fu, C. & Carter, C. E. (1990). Detection of a circulating antigen in human schistosomiasis japonica using a monoclonal antibody. American Journal of Tropical Medicine and Hygiene 42, 347–51.CrossRefGoogle ScholarPubMed
Grimaud, J. A., Borojevic, R. & Santos, H.Araujo Dos (1976). Schistosomal pigment in human and murine infections with Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 73–7.CrossRefGoogle ScholarPubMed
Hassan, M. M., Badawi, M. A. & Strand, M. (1992). Circulating schistosomal antigen in diagnosis and assessment of cure in individuals infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 46, 737–44.CrossRefGoogle ScholarPubMed
Katz, N., Chaves, A. & Pellegrino, J. (1972). A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Revista do Institute de medicina tropical de São Paulo 14, 397–400.Google ScholarPubMed
Kestens, L., Mangelschots, K., Van Marck, E. A., Gigase, P. L. & Deelder, A. M. (1988). Schistosoma mansoni: impaired clearance of model immune complexes consisting of circulating anodic antigen and monoclonal IgG1 in infected mice. Parasitology Research 74, 356–62.CrossRefGoogle ScholarPubMed
Köster, B. & Strand, M. (1994). Schistosoma mansoni: immunolocalization of two different fucose-containing carbohydrate epitopes. Parasitology 108, 433–46.CrossRefGoogle ScholarPubMed
Mott, K. E. & Cline, B. L. (1980). Advances in epidemiology survey methodology and techniques in schistosomiasis. Bulletin of the World Health Organization 58, 639–47.Google Scholar
Nash, T. E. (1974). Localization of the circulating antigen within the gut of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 23, 1085–7.CrossRefGoogle ScholarPubMed
Nash, T. E., Prescott, B. & Neva, F. A. (1974). The characteristics of a circulating antigen in schistosomiasis. Journal of Immunology 112, 1500–7.CrossRefGoogle ScholarPubMed
Nourel, Din M. S., Kornelis, D., Van Zeyl, R. J. & Deelder, A. M. (1994 a). Immunologic characterization of two monoclonal antibodies reactive with repetitive carbohydrate epitopes of circulating Schistosoma mansoni egg antigen. American Journal of Tropical Medicine and Hygiene 50, 487–98.CrossRefGoogle Scholar
Nourel, Din M. S., Nibbeling, R., Rotmans, J. P., Polderman, A. M., Krijger, F. W. & Deelder, A. M. (1994 b). Quantitative determination of circulating soluble egg antigen in urine and serum of Schistosoma mansoni-infected individuals using a combined two-site enzyme-linked immunosorbent assay. American Journal of Tropical Medicine and Hygiene 50, 585–94.CrossRefGoogle Scholar
Stenger, R. J., Warren, K. S. & Johnson, E. A. (1967). An electron microscopic study of the liver parenchyma and schistosome pigment in murine hepatosplenic schistosomiasis mansoni. American Journal of Tropical Medicine and Hygiene 16, 473–82.CrossRefGoogle ScholarPubMed
Strand, M., McMillan, A. & Pan, X.-Q. (1982). Schistosoma mansoni: Reactivity with infected human sera and monoclonal antibody characterization of a glycoprotein in different developmental stages. Experimental Parasitology 54, 145–56.CrossRefGoogle ScholarPubMed
Terr, A. I. (1994). Inflammation. In Basic and Clinical Immunology, 8th Edn, (ed. Stites, D. P., Terr, A. I. & Parslow, T. G.), pp. 137–150. Norwalk: Appleton & Lange.Google Scholar
Van Marck, E. (1975). Presence of the circulating polysaccharide antigen in the liver of mice infected with Schistosoma mansoni. Annales de la Societe beige de Médecine tropicale 55, 373–7.Google ScholarPubMed
Van Marck, E. A. (1983). The glomerulopathy associated with Schistosoma mansoni infection. An experimental study in the mouse. Ada Leidensia 50, 1–123.Google ScholarPubMed
Van Marck, E. A., Kestens, L., Stocker, S., Grimaud, J. A., Gigase, P. L. & Deelder, A. M. (1983). Fibrosis around schistosomal egg antigen-coated beads in the liver of mice. Contributions to Microbiology and Immunology 7, 251–9.Google ScholarPubMed
Von Lichtenberg, F., Bawden, M. P. & Shealey, S. H. (1974). Origin of circulating antigen from the schistosome gut. An immunofluorescent study. American Journal of Tropical Medicine and Hygiene 23, 1088–91.CrossRefGoogle ScholarPubMed
Weiss, J. B. & Strand, M. (1985). Characterization of developmentally regulated epitopes of Schistosoma mansoni egg glycoprotein antigens. Journal of Immunology 135, 1421–9.CrossRefGoogle ScholarPubMed
Weller, P. F. (1991). The immunobiology of eosinophils. The New England Journal of Medicine 324, 1110–18.Google ScholarPubMed