Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-22T06:57:53.788Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunodiagnosis of taeniasis by coproantigen detection

Published online by Cambridge University Press:  06 April 2009

J. C. Allan ,
G. Avila ,
J. Garcia Noval ,
A. Flisser  and
P. S. Craig
J. C. Allan
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
G. Avila
Affiliation:
Instituto de Investigaciones Biomedicas, Apartado Postal 70228, Ciudad Universitaria, 04150, Mexico D.F.
J. Garcia Noval
Affiliation:
Centro de Investigaciones de Ciencias de la Salud, Universidad de San Carlos de Guatemala, Guatemala City, Guatemala C.A.
A. Flisser
Affiliation:
Instituto de Investigaciones Biomedicas, Apartado Postal 70228, Ciudad Universitaria, 04150, Mexico D.F.
P. S. Craig
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
Get access Rights & Permissions [Opens in a new window]

Summary

Immunodiagnostic tests for Taenia-specific faecal antigen based on polyclonal rabbit antisera against Taenia saginata or Taenia solium proglottid extracts in capture-type ELISA assays have been developed. Taenia-specific antigen was detected in detergent-solubilized faecal extracts from T. solium- and T. saginata-infected hosts. Coproantigen from T. solium-infected hamsters did not cross-react with faeces from rodents infected with Hymenolepis diminuta, H. citelli, H. micro-stoma, Necator americanus, Strongyloides ratti or Nematospiroides dubius and faeces from uninfected animals. When the T. saginata-capture ELISA was tested with faecal samples positive for T. solium antigen, no cross-reactions were obtained. However, faecal samples from humans infected with T. solium or T. saginata, including some with extremely low egg counts, were cross-reactive by either test. Nevertheless, considerably higher O.D. values were obtained with stool samples from Taenia patients compared to Hymenolepis nana-infected or uninfected individuals. Two individuals, infected with Taenia sp. and positive for coproantigens by ELISA, became antigen-negative 6 days after treatment with Niclosamide. The possibility of developing species-specific immunodiagnostic tests for human taeniasis through coproantigen detection is discussed.

Keywords

immunodiagnosticcoproantigenTaenia saginataTaenia soliumgolden hamster
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 473 - 477
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allan, J. C. & Craig, P. S. (1989). Corproantigens in gut tapeworm infections: Hymenolepis diminuta in rats. Parasitology Research 76, 68–73.CrossRefGoogle ScholarPubMed
Flentje, V. B. & Padelt, H. (1981). Wert einer serologischen Diagnostik der Taenia saginata Infestation des Menschen. Angewandte Parasitologie 22, 65–8.Google Scholar
Flisser, A. (1985). Cysticercosis: a major threat to human health and livestock production. Food Technology 39, 61–4.Google Scholar
Gasser, R. B., Lightowlers, M. W., Obendorf, D. F., Jenkins, D. J. & Rickard, M. D. (1988). Evaluation of a serological test system for the diagnosis of natural Echinococcus granulosus infection in dogs using E. granulosus protoscolex and oncosphere antigens. Australian Veterinary Journal 65, 369–73.CrossRefGoogle ScholarPubMed
Heath, D. D., Lawrence, B., Glennie, A. & Twaalfhoven, H. (1985). The use of excretory and secretory antigens of the scolex of Taenia ovis for the serodiagnosis of infection in dogs. Journal of Parasitology 71, 192–9.CrossRefGoogle ScholarPubMed
Jenkins, D. J. & Rickard, M. D. (1985). Specific antibody responses to Taenia hydatigena, Taenia pisiformis and Echinococcus granulosus infection in dogs. Australian Veterinary Journal 62, 72–8.CrossRefGoogle ScholarPubMed
Jenkins, D. J. & Rickard, M. D. (1986). Specific antibody responses in dogs experimentally infected with Echinococcus granulosus. American Journal of Tropical Medicine and Hygiene 35, 345–9.CrossRefGoogle ScholarPubMed
Machnicka-Roguska, B. & Zwierz, C. (1970). Intradermal test with antigenic fractions in Taenia saginata infection. Ada Parasitologica Polonica 18, 293–9.Google Scholar
Sarti-Gutierrez, E. J., Schantz, P. M., Lara-Aguillera, R., Gomez Dandoy, H. & Flisser, A. (1988). Taenia solium taeniasis and cysticercosis in a Mexican village. Tropical Medicine and Parasitology 39, 194–8.Google Scholar
Verster, A. (1974). The golden hamster as a definitive host of Taenia solium and Taenia saginata. Onderspoort Journal of Veterinary Research 41, 23–8.Google ScholarPubMed
Viscidi, R., Laughon, B. E., Hanvanich, M. & Bartlett, R. H. (1984). Improved enzyme immunoassays for the detection of antigens in fecal specimens. Investigation and correction of interfering factors. Journal of Immunological Methods 67, 129–43.CrossRefGoogle ScholarPubMed