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Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection of Paramphistomum gracile circulating 16 kDa antigen

Published online by Cambridge University Press:  21 February 2017

PANAT ANURACPREEDA ,
KULLANID TEPSUPORNKUL  and
RUNGLAWAN CHAWENGKIRTTIKUL
PANAT ANURACPREEDA*
Affiliation:
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakorn Pathom 73170, Thailand Division of Agricultural Science, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi, Thailand
KULLANID TEPSUPORNKUL
Affiliation:
Division of Agricultural Science, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi, Thailand
RUNGLAWAN CHAWENGKIRTTIKUL
Affiliation:
Department of Microbiology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand
*
*Corresponding author. Division of Agricultural Science, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi 71150, Thailand. E-mail: [email protected], [email protected]
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Summary

In this study, we have produced a monoclonal antibody (MoAb) against 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for the detection of circulating 16 kDaAg in the serum and fecal samples from cattle naturally infected with P. gracile. MoAb 1D10 was immobilized on a microtitre plate, and the antigen in the samples was captured and detected with biotinylated rabbit anti-16 kDaAgPg antibody. The lower detection limit of sandwich ELISA was 3·5 pg mL−1, and no cross-reaction with other parasite antigens was evaluated. The reliability of the assay was examined using the serum and fecal samples from cattle naturally infected with P. gracile, Fasciola gigantica, Moniezia benedeni, Trichuris sp., Strongyloides sp., strongylids and non-infected animals. The sandwich ELISA showed the sensitivity, specificity and accuracy at 98·33, 100 and 99·55% (serum samples), and 96·67, 100 and 99·09% (fecal samples). Therefore, this detection method is a rapid and excellent potential assay for the accurate diagnosis of paramphistomosis.

Keywords

Paramphisomum gracileparamphistomosis16 kDa antigenmonoclonal antibodysandwich ELISAimmunodiagnosis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 7 , June 2017 , pp. 899 - 903
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Copyright
Copyright © Cambridge University Press 2017 

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References

REFERENCES

Anuracpreeda, P., Wanichanon, C., Chaithirayanon, K., Preyavichyapugdee, N. and Sobhon, P. (2006). Distribution of 28·5 kDa antigen in tegument of adult Fasciola gigantica . Acta Tropica 100, 31–40.Google Scholar
Anuracpreeda, P., Wanichanon, C. and Sobhon, P. (2008). Paramphistomum cervi:antigenic profile of adults as recognized by infected cattle sera. Experimental Parasitology 118, 203–207.CrossRefGoogle ScholarPubMed
Anuracpreeda, P., Wanichanon, C., Chawengkirtikul, R., Chaithirayanon, K. and Sobhon, P. (2009 a). Fasciola gigantica: immunodiagnosis of fasciolosis by detection of circulating 28·5 kDa tegumental antigen. Experimental Parasitology 123, 334–340.CrossRefGoogle ScholarPubMed
Anuracpreeda, P., Wanichanon, C. and Sobhon, P. (2009 b). Fasciola gigantica: immunolocalization of 28·5 kDa antigen in the tegument of metacercaria and juvenile fluke. Experimental Parasitology 122, 75–83.Google Scholar
Anuracpreeda, P., Songkoomkrong, S., Sethadavit, M., Chotwiwatthanakun, C., Tinikul, Y. and Sobhon, P. (2011). Fasciola gigantica: production and characterization of a monoclonal antibody against recombinant cathepsin B3. Experimental Parasitology 127, 340–345.Google Scholar
Anuracpreeda, P., Panyarachun, B., Ngamniyom, A., Tinikul, Y., Chotwiwatthanakun, C., Poljaroen, J. and Sobhon, P. (2012). Fischoederius cobboldi: a scanning electronmicroscopy investigation of surface morphology of adult rumen fluke. Experimental Parasitology 130, 400–407.Google Scholar
Anuracpreeda, P., Chawengkirtikul, R., Tinikul, Y., Poljaroen, J., Chotwiwatthanakun, C. and Sobhon, P. (2013 a). Diagnosis of Fasciola gigantica infection using a monoclonal antibody-based sandwich ELISA for detection of circulating cathepsin B3 protease. Acta Tropica 127, 38–45.CrossRefGoogle ScholarPubMed
Anuracpreeda, P., Poljaroen, J., Chotwiwatthanakun, C., Tinikul, Y. and Sobhon, P. (2013 b). Antigenic components, isolation and partial characterization of excretion-secretion fraction of Paramphistomum cervi . Experimental Parasitology 133, 327–333.Google Scholar
Anuracpreeda, P., Srirakam, T., Pandonlan, S., Changklungmoa, N., Chotwiwatthanakun, C., Tinikul, Y., Poljaroen, J., Meemon, K. and Sobhon, P. (2014). Production and characterization of a monoclonal antibody against recombinant cathepsin L1 of Fasciola gigantica . Acta Tropica 135, 1–9.Google Scholar
Anuracpreeda, P., Phutong, S., Ngamniyom, A., Panyarachun, B. and Sobhon, P. (2015). Surface topography and ultrastructural architecture of the tegument of adult Carmyerius spatiosus Brandes, 1898. Acta Tropica 143, 18–28.Google Scholar
Anuracpreeda, P., Chawengkirttikul, R. and Sobhon, P. (2016 a). Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile . Parasite Immunology 38, 431–438.CrossRefGoogle ScholarPubMed
Anuracpreeda, P., Chawengkirttikul, R. and Sobhon, P. (2016 b). Immunodiagnosis of Fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and immunochromatographic assay for detection of circulating cathepsin L1 protease. PLoS ONE 11, 1–22. e0145650.CrossRefGoogle ScholarPubMed
Anuracpreeda, P., Chawengkirttikul, R. and Sobhon, P. (2016 c). Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein. Parasitology 143, 1369–1381.Google Scholar
Anuracpreeda, P., Chawengkirttikul, R. and Sobhon, P. (2016 d). Surface histology, topography, and ultrastructure of the tegument of adult Orthocoelium parvipapillatum (Stiles & Goldberger, 1910). Parasitology Research 115, 2757–2769.Google Scholar
Anuracpreeda, P., Anuracpreeda, P., Watthanadirek, A., Chawengkirttikul, R. and Sobhon, P. (2017). Production and characterization of a monoclonal antibody specific to 16 kDa antigen of Paramphistomum gracile . Parasitology Research 116, 167–175.Google Scholar
Anuracpreeda, P., Chankaew, K., Puttarak, P., Koedrith, P., Chawengkirttikul, R., Panyarachun, B., Ngamniyom, A., Chanchai, S. and Sobhon, P. (2016 e). The anthelmintic effects of the ethanol extract of Terminalia catappa L. leaves against the ruminant gut parasite, Fischoederius cobboldi . Parasitology 143, 421–433.CrossRefGoogle ScholarPubMed
Bida, S. A. and Schillhorn van Veen, Tj. (1977). Enteric paramphistomiasis in yankasa sheep. Tropical Animal Health and Production 9, 21–23.Google Scholar
Chethanon, U., Ausavamatha, P. and Piriyayon, S. (1985). Parasitological survey of cattle in the south of Thailand. In Proceedings of the 4th Annual Livestock Conference, 3–5 July 1985. Department Livestock Development, pp. 281–290.Google Scholar
Demerdash, Z. A., Diab, T. M., Aly, I. R., Mohamed, S. H., Mahmoud, F. S., Zoheiry, M. K., Mansour, W. A., Attia, M. E. and El-Bassiouny, A. E. (2011). Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool. Parasites and Vectors 4, 176.Google Scholar
Espino, A. and Finlay, C. (1994). Sandwich enzyme-liked immunosorbent assay for detection of excretory/secretory antigens in humans with fascioliasis. Journal of Clinical Microbiology 32, 190–193.Google Scholar
Estuningsih, E., Spithill, T., Raadsma, H., Law, R., Adiwinata, G., Meeusen, Els. and Piedrafita, D. (2009). Development and application of a fecal antigen diagnostic sandwich ELISA for estimating prevalence of Fasciola gigantica in cattle in Central Java, Indonesia. The Journal of Parasitology 95, 450–455.CrossRefGoogle ScholarPubMed
Galen, R. S. (1980). Predictive value and efficiency of laboratory testing. Pediatric Clinics of North America 27, 861–869.Google Scholar
Gupta, P. P., Singh, B. and Dutt, S. C. (1978). A note on amphistomiasis in an adult buffalo. The Indian Veterinary Journal 55, 491–492.Google Scholar
Hanna, R. E. B., Williamson, D. S., Mattison, R. G. and Nizami, W. A. (1988). Seasonal reproduction in Paramphistomum epiclitum and Gastrothylax crumenifer, rumen paramphistomes of the Indian water buffalo, and comparison with the biliary paramphistome Gigantocotyle explanatum . International Journal for Parasitology 18, 513–521.CrossRefGoogle ScholarPubMed
Horak, I. G. (1971). Paramphistomiasis of domestic ruminants. Advance in Parasitology 9, 33–71.Google Scholar
Langley, R. J. and Hillyer, G. V. (1989). Detection of circulating parasite antigen in murine fascioliasis by two-site enzyme-linked immunosorbent assays. American Journal of Tropical Medicine and Hygiene 41, 472–478.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randal, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Chemical Biology 193, 265–275.Google Scholar
Mezo, M., Gonzalez-Warleta, M. and Ubeira, F. M. (2004). An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3). Journal of Parasitology 90, 845–852.Google Scholar
Nikitin, V. F. (1979). The course of acute enzootic amphistomiasis in claves in the lower Volga region. Helminthological Abstract Series A 48, 463.Google Scholar
Panyarachun, B., Sobhon, P., Yotsawan, T., Chotwiwatthanakun, C., Anupunpisit, V. and Anuracpreeda, P. (2010). Paramphistomum cervi: surface topography of the tegument of adult fluke. Experimental Parasitology 125, 95–99.CrossRefGoogle ScholarPubMed
Panyarachun, B., Ngamniyom, A., Sobhon, P. and Anuracpreeda, P. (2013). Morphology and histology of the adult Paramphistomum gracile Fischoeder, 1901. Journal of Veterinary Science 14, 425–432.Google Scholar
Prasitirat, P., Chompoochan, T., Nithiuthai, S., Wongkasemjit, S., Punmamoamg, T., Pongrut, P., Chinone, S. and Itagaki, H. (1997). Prevalence of amphistomes of cattle in Thailand. Parasitology (Hungary) 29/30, 27–32.Google Scholar
Zheng, H. J., Tao Zheng-Hou, C. W. and Pessens, W. F. (1990). Comparison of dot-ELISA with sandwich ELISA for the detection of circulating antigens in patients with Bancroftian filariasis. American Journal of Tropical Medicine and Hygiene 42, 546–549.Google Scholar