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Immunocytochemical demonstration of neuropeptides in the central nervous system of the roundworm, Ascaris suum (Nematoda: Ascaroidea)

Published online by Cambridge University Press:  06 April 2009

D. J. A. Brownlee ,
I. Fairweather ,
C. F. Johnston ,
D. Smart ,
C. Shaw  and
D. W. Halton
D. J. A. Brownlee
Affiliation:
Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
I. Fairweather*
Affiliation:
Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
C. F. Johnston
Affiliation:
Comparative Neuroendocrinology Research Group, School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
D. Smart
Affiliation:
Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
C. Shaw
Affiliation:
Comparative Neuroendocrinology Research Group, School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
D. W. Halton
Affiliation:
Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
*
*Dr I. Fairweather, School of Biology and Biochemistry, The Queen's University of Belfast, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland
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Summary

The localization and distribution of neuropeptides in the central nervous system of the pig roundworm, Ascaris suum, have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. Antisera to 25 vertebrate peptides and two invertebrate peptides were used to screen the worm for immunoreactivity (IR). Immuno-staining was obtained with antisera to pancreatic polypeptide (PP), peptide YY (PYY), neuropeptide Y (NPY), gastrin, cholecystokinin (CCK), substance P (SP), atrial natriuretic peptide (ANP), salmon gonadotropin-releasing hormone (SGnRH), mammalian gonadotropin-releasing hormone (MGnRH), chromogranin A (CGA) and FMRFamide. The most extensive patterns of IR occurred with antisera to PYY, FMRFamide and gastrin. IR was evident in nerve cells and fibres in the ganglia associated with the anterior nerve ring and in the main nerve cords and their commissures; IR to FMRFamide also occurred in the posterior nerve ring. Immunostaining for the other peptides was confined to the nerve cords, with the number of immunoreactive nerve fibres varying from peptide to peptide.

Keywords

Ascaris suumNematodaneuropeptidesimmunocytochemistryconfocal microscopy
Type
Research Article
Information
Parasitology , Volume 106 , Issue 3 , April 1993 , pp. 305 - 316
Copyright
Copyright © Cambridge University Press 1993

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