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Immunization of mice with gamma-irradiated intramuscularly injected schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

Q. D. Bickle ,
M. G. Taylor ,
M. J. Doenhoff  and
G. S. Nelson
Q. D. Bickle
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
M. G. Taylor
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
M. J. Doenhoff
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
G. S. Nelson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
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Summary

The parameters involved in the induction of resistance against Schistosoma mansoni by injection of irradiated, artificially transformed schistosomula were studied in mice. Single intramuscular injections of 500 schistosomula exposed to radiation doses in the range 2·3 to 160 krad. resulted in significant protection (in the range 20 to 50% as assessed by reduced worm burdens) against a challenge infection administered at intervals from 3 to 24 weeks post-vaccination. However, schistosomula irradiated with 20 krad. consistently resulted in better protection than those exposed to either higher or lower radiation doses despite the persistence of stunted adults from the infections irradiated with 2·3 krad. Vaccination with 40 krad. schistosomula resulted in significant protection in terms of reduced worm and tissue egg burdens and increased survival following lethal challenge. Varying the number of irradiated schistosomula, the frequency and route of their administration, the site of challenge and the strain of host all failed to enhance the level of resistance. However, percutaneously applied, irradiated cercariae were found to be more effective in stimulating resistance (60%) than intramuscularly injected, irradiated schistosomula (40%).

Type
Research Article
Information
Parasitology , Volume 79 , Issue 2 , October 1979 , pp. 209 - 222
Copyright
Copyright © Cambridge University Press 1979

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References

REFERENCES

Bickle, Q. D., Dobinson, T. & James, E. R. (1979). The effects of gamma irradiation on migration and survival of S. mansoni schistosomula in mice. Parasitology 79, 223–30.CrossRefGoogle Scholar
Bickle, Q. D., Taylor, M. G., James, E. R., Nelson, G. S., Hussein, M. F., Andrews, B. J., Dobinson, A. R. & Marshall, T. F. de C. (1979). Further observations on immunization of sheep against Schistosoma matteei and S. bovis using irradiation-attenuated schistosomula of homologous and heterologous species. Parasitology 78, 185–93.CrossRefGoogle ScholarPubMed
Brink, L. H., McLaren, D. J. & Smithers, S. R. (1977). Schistosoma mansoni: a comparative study of artificially transformed schistosomula and schistosomula recovered after cercarial penetration of isolated skin. Parasitology 74, 73–86.CrossRefGoogle ScholarPubMed
Colley, D. G. & Wikel, S. K. (1974). Schistosoma mansoni: simplified method for the production of schistosomula. Experimental Parasitology 35, 44–51.CrossRefGoogle Scholar
Doenhoff, M., Bickle, Q., Long, E., Bain, J. & McGregor, A. (1978). Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. I. Demonstration of resistance to reinfection using a model system that involves perfusion of mice within three weeks of challenge. Journal of Helminthology 52, 173–86.CrossRefGoogle Scholar
Erickson, D. G. & Caldwell, W. L. (1965). Acquired resistance in mice and rats after exposure to gamma-irradiated cercariae. American Journal of Tropical Medicine and Hygiene 14, 566–73.CrossRefGoogle Scholar
Eveland, L. K. & Morse, S. J. (1978). Schistosoma mansoni: infectivity and immunizing effects of in vitro derived schistosomula attenuated by x-irradiation. Experimental Parasitology 45, 19–25.CrossRefGoogle ScholarPubMed
Hsü, H. F., Hsü, S. Y. Li & Osborne, J. W. (1965). Immunization against Schistosoma japonicum in rhesus monkeys. Nature, London 206, 1338–40.CrossRefGoogle ScholarPubMed
Hsü, S. Y. L., Hsü, H. F. & Osborne, J. W. (1969). Immunization of rhesus monkeys against schistosome-infection by cercariae exposed to high doses of x-irradiation. Proceedings of the Society for Experimental Biology and Medicine 131, 1146–9.CrossRefGoogle Scholar
James, E. R. & Farrant, J. (1977). Recovery of infective Schistosoma mansoni schistosomula from liquid nitrogen: a step towards storage of a live schistosomiasis vaccine. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 498–500.CrossRefGoogle ScholarPubMed
James, E. R. & Taylor, M. G. (1976). Transformation of cercariae to schistosomula: a quantitative comparison of the transformation techniques and of infectivity by different injection routes of the organisms produced. Journal of Helminthology 50, 223–33.CrossRefGoogle ScholarPubMed
Minard, P., Dean, D. A., Vannier, W. E. & Murrell, K. D. (1978). Effect of immunization on migration of Schistosoma mansoni through lungs. American Journal of Tropical Medicine and Hygiene 27, 87–93.CrossRefGoogle ScholarPubMed
Minard, P., Dean, D. A., Jacobson, R. H., Vannier, W. E. & Murrell, K. D. (1978). Immunization of mice with cobalt-60 irradiated Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 27, 76–86.CrossRefGoogle ScholarPubMed
Perlowagora-Szumlewicz, A. (1964). O papel das cercárias atenuades na imunizaçao efetiva contra o Schistosoma mansoni (Studies on acquired resistance to Schistosoma mansoni in mice exposed to x-irradiated cercariae). Revista Brazileira de Malariologia e Doenças Tropicas (Rio de Janeiro) 16, 502–25. (English summary.)Google Scholar
Perlowagora-Szumlewicz, A. & Olivier, L. J. (1963). Schistosoma mansoni: development of challenge infections in mice exposed to irradiated cercariae. Science 140, 411–12.CrossRefGoogle Scholar
Phillips, S. M., Reid, W. A., Doughty, B. & Khoury, P. B. (1978). The cellular and humoral immune response to Schistosoma mansoni infections in inbred rats. V. Prerequisite mechanisms for the development of optimal protective immunity. Cellular Immunology 38, 239–54.CrossRefGoogle ScholarPubMed
Radke, M. G. & Sadun, E. H. (1963). Resistance produced in mice by exposure to irradiated Schistosoma mansoni cercariae. Experimental Parasitology 13, 134–43.CrossRefGoogle Scholar
Sadun, E. H., Bruce, J. I. & Macomber, P. B. (1964). Parasitologic, pathologic and serologic reactions to Schistosoma mansoni in monkeys exposed to irradiated cercariae. American Journal of Tropical Medicine and Hygiene 13, 548–57.CrossRefGoogle ScholarPubMed
Smith, M., Clegg, J. A., Kusel, J. R. & Webbe, G. (1975). Lung inflammation in immunity to Schistosoma mansoni. Separatum Experimentia 31, 595–6.CrossRefGoogle ScholarPubMed
Smithers, S. R. (1962). Stimulation of acquired resistance to Schistosoma mansoni in monkeys: role of eggs and worms. Experimental Parasitology 12, 263–73.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965 a). Acquired resistance to experimental infections of Schistosoma mansoni in the albino rat. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 517–33.CrossRefGoogle Scholar
Smithers, S. R. & Terry, R. J. (1965 b). The infection of laboratory hosts with cercariae of Schistosoma mansoni. Experimental Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1969). Immunity in schistosomiasis. Annals of the New York Academy of Science 160, 826–40.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. (1974). Schistosoma mansoni: cercaria to schistosomula. Advances in Parasitology 12, 115.CrossRefGoogle Scholar
Taylor, M. G., James, E. R., Bickle, Q. D., Doenhoff, J. J., Nelson, G. S., Hussein, M. F. & Bushara, H. O. (1977). Vaccination in schistosomiasis. In Immunity in Parasitic Diseases, INSERM, vol. 72, pp. 29–306.Google Scholar
Taylor, M. G., James, E. R., Nelson, G. S., Bickle, Q. D., Andrews, B. J., Dobinson, A. R. & Webbe, G. (1976). Immunisation of baboons against Schistosoma mansoni using irradiated cercariae and schistosomula and non-irradiated cercariae. Journal of Helminthology 50, 215–21.CrossRefGoogle Scholar
Taylor, M. G., James, E. R., Nelson, G. S., Bickle, Q. D., Dunne, D. W. & Webbe, G. (1976). Immunisation of sheep against Schistosoma mattheei using either irradiated cercariae or irradiated schistosomula. Journal of Helminthology 50, 1–9.CrossRefGoogle ScholarPubMed
Villella, J. B., Gomberg, H. J. & Gould, S. E. (1961). Immunization to Schistosoma mansoni in mice inoculated with radiated cercariae. Science 134, 1073–5.CrossRefGoogle ScholarPubMed