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Identification of Leishmania infantum in blood donors from endemic regions for visceral leishmaniasis

Published online by Cambridge University Press:  04 November 2020

Loren Queli Pereira Open the ORCID record for Loren Queli Pereira [Opens in a new window] ,
Márcia Maria Ferreira-Silva Open the ORCID record for Márcia Maria Ferreira-Silva [Opens in a new window] ,
Cristhianne Molinero Andrade Ratkevicius Open the ORCID record for Cristhianne Molinero Andrade Ratkevicius [Opens in a new window] ,
César Gómez-Hérnandez Open the ORCID record for César Gómez-Hérnandez [Opens in a new window] ,
Fernanda Bernadelli De Vito Open the ORCID record for Fernanda Bernadelli De Vito [Opens in a new window] ,
Sarah Cristina Sato Vaz Tanaka Open the ORCID record for Sarah Cristina Sato Vaz Tanaka [Opens in a new window] ,
Virmondes Rodrigues Júnior Open the ORCID record for Virmondes Rodrigues Júnior [Opens in a new window]  and
Helio Moraes-Souza Open the ORCID record for Helio Moraes-Souza [Opens in a new window]
Loren Queli Pereira*
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Márcia Maria Ferreira-Silva
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Cristhianne Molinero Andrade Ratkevicius
Affiliation:
Laboratory of Immunology, Triângulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil
César Gómez-Hérnandez
Affiliation:
Laboratory of Immunology, Triângulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil
Fernanda Bernadelli De Vito
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Sarah Cristina Sato Vaz Tanaka
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Virmondes Rodrigues Júnior
Affiliation:
Laboratory of Immunology, Triângulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil
Helio Moraes-Souza
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
*
Author for correspondence: Helio Moraes-Souza, E-mail: [email protected]
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Abstract

Visceral leishmaniasis is an endemic protozoonosis observed in over 60 countries, with over 500 000 new cases recorded annually. Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus on the best method for its identification. Recent studies have presented molecular techniques as viable identification methods, with good sensitivity and specificity indices in asymptomatic individuals. Therefore, we aimed to use molecular methods to assess their effectiveness in identifying the presence of asymptomatic infection by Leishmania infantum (L. infantum) individuals from endemic regions of Brazil. Screening was performed by real-time polymerase chain reaction (qPCR) and confirmed by sequencing the cytochrome B gene. Of the 127 samples [from 608 blood donors who had participated in a previous study, of which 34 were positive by the enzyme-linked immunosorbent assay (ELISA) rK39] tested by qPCR, 31 (24.4%) were positive. In the sequencing of 10 qPCR-positive samples, five were identified as L. infantum. Complimentary samples of the ELISA rK39 and conventional PCR showed only reasonable and low agreement with qPCR, respectively. The qPCR confirmed the presence of infection in five of the 10 sequenced samples, ELISA confirmed three, and the conventional PCR confirmed none.

Keywords

Asymptomatic visceral leishmaniasisblood donorsCytBLeishmania infantumqPCR
Type
Research Article
Information
Parasitology , Volume 148 , Issue 1 , January 2021 , pp. 110 - 114
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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