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Identification of common surface antigens among Babesia bigemina isolates by using monoclonal antibodies

Published online by Cambridge University Press:  06 April 2009

J. V. Figueroa ,
G. M. Buening ,
D. A. Kinden  and
T. J. Green
J. V. Figueroa
Affiliation:
Departments of Veterinary MicrobiologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
G. M. Buening
Affiliation:
Departments of Veterinary MicrobiologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
D. A. Kinden
Affiliation:
Veterinary PathologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
T. J. Green
Affiliation:
Departments of Veterinary MicrobiologyCollege of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
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Summary

A group of monoclonal antibodies (MAb) was used to immunochemically characterize Babesia bigemina surface components. Two surface reactive MAb by an IFAT test were subsequently shown to bind to epitopes on the external surface of the parasite plasma membrane as evidenced by immunoelectron microscopy. Parasite components with relative sizes of 68, 62, 60, 58, 56, 54, 51, 49, 48, 47, 43, 36 kDa were identified with the group of MAb in at least 6 geographically different B. bigemina isolates. The antigenic components were demonstrated to be species-specific and apparently predominant. Both polyclonal antibodies (immune bovine serum) and highly diluted MAb specifically reacted with such bands producing a strong signal in Western blots.

Keywords

Babesia bigeminasurface antigensmonoclonal antibodiesimmunoblottingimmunoprecipitationimmunoelectron microscopy
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 161 - 175
Copyright
Copyright © Cambridge University Press 1990

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References

Bonner, W. M. & Laskey, R. A. (1974). A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46, 83–8.CrossRefGoogle Scholar
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein – dye binding. Analytical Biochemistry 72, 248–54.CrossRefGoogle ScholarPubMed
Bruck, C., Drebin, J. A., Glineur, C. & Portetelle, D. (1986). Purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-gel blue chromatography. In Methods in Enzymology, vol. 121 (ed. Langone, J. J. & Van Vunakis, H.), pp. 587–96. New York: Academic Press.Google Scholar
Callow, L. L. (1967). Sterile immunity, coinfectious immunity and strain differences in Babesia bigemina infections. Parasitology 57, 455–65.CrossRefGoogle ScholarPubMed
Callow, L. L., Mellors, L. T. & McGregor, W. (1979). Reduction in virulence of Babesia bovis due to rapid passage in splenectomized cattle. International Journal for Parasitology 9, 333–8.CrossRefGoogle ScholarPubMed
Charoenvit, Y., Leef, M. F., Yuan, L. F., Sedegah, M. & Beaudoin, R. L. (1987). Characterization of Plasmodium yoelli monoclonal antibodies directed against stage-specific sporozoite antigens. Infection and Immunity 55, 604–8.CrossRefGoogle ScholarPubMed
Christensson, D. A. & Moren, T. (1987). Seroresponse (IgG) after vaccination and natural infection of cattle with Babesia divergens. Acta Veterinaria Scandinavica 28, 393–402.CrossRefGoogle ScholarPubMed
Curnow, J. A. (1973). The use of a slide agglutination test to demonstrate antigenic differences between Babesia bigemina parasites. Australian Veterinary Journal 49, 290–3.CrossRefGoogle ScholarPubMed
Dalgliesh, R. J., Callow, L. L., Mellors, L. T. & McGregor, W. (1981). Development of a highly infective Babesia bigemina vaccine of reduced virulence. Australian Veterinary Journal 57, 8–11.CrossRefGoogle ScholarPubMed
De Waele, M. (1984). Haematological electron immunochemistry. Detection of cell surface antigens with monoclonal antibodies. In Immunolabeling for Electron Microscopy (ed. Polak, J. M. & Varndell, I. M.), pp. 267–88. Amsterdam: Elsevier Science Publishers.Google Scholar
Gill, A. C., Cowman, A. F., Stewart, N. P., Kemp, D. J. & Timms, P. (1987). Babesia bovis: Molecular and biological characteristics of cloned parasite lines. Experimental Parasitology 63, 180–8.CrossRefGoogle ScholarPubMed
Goff, W. L., Davis, W. C., Palmer, C. H., McElwain, T. F., Johnson, W. C., Bailey, J. F. & McGuire, T. C. (1988). Identification of Babesia bovis merozoite surface antigens by using immune bovine sera and monoclonal antibodies. Infection and Immunity 56, 2363–8.CrossRefGoogle ScholarPubMed
Goodger, B. V. (1971). Preparation and preliminary assessment of purified antigens in the passive haemagglutination test for bovine babesiosis. Australian Veterinary Journal 47, 251–6.CrossRefGoogle ScholarPubMed
Goodger, B. V., Commins, M. A., Wright, I. G. & Mirre, C. B. (1984). Babesia bovis: Vaccination of cattle against heterologous challenge with fractions of lysate from infected erythrocytes. Zeitschrift für Parasitenkunde 70, 321–9.CrossRefGoogle ScholarPubMed
Goodger, B. V., Commins, M. A., Wright, I. G., Mirre, G. B., Waltisbuhl, D. J. & White, M. (1986). Babesia bovis: Vaccination trial with a dominant immunodiffusion antigen in splenectomized calves. Zeitschrift für Parasitenkunde 72, 715–22.CrossRefGoogle Scholar
Gravely, S. M., Smith, R. D., Erp, E. E.Canto, G. J.Aikawa, M., Osorno, B. M. & Ristic, M. (1979). Bovine babesiosis: Partial purification and characterization of blood culture-derived Babesia bovis. International Journal for Parasitology 9, 591–8.CrossRefGoogle ScholarPubMed
Hall, R., McBride, J., Morgan, G., Tait, A., Zolg, J. W.Walliker, D. & Scaife, J. (1983). Antigens of the erythrocytic stages of the human malaria parasite Plasmodium falciparum detected by monoclonal antibodies. Molecular and Biochemical Parasitology 7, 247–65.CrossRefGoogle ScholarPubMed
Howard, R. F., Stanley, H. A., Campbell, G. H. & Reese, R. T. (1984). Proteins responsible for a punctate fluorescence pattern in Plasmodium falciparum merozoites. American Journal of Tropical Medicine and Hygiene 33, 1055–9.CrossRefGoogle ScholarPubMed
James, M. A. (1984). Immunology of Babesia infections. In Malaria and Babesiosis Research Findings and Control Measures (ed. Ristic, M.Ambroise-Thomas, P. & Kreier, J. P.), pp. 53–63. Boston: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Kahl, L. P., Anders, R. F., Rodwell, B. J., Timms, P. & Mitchell, G. F. (1982). Variable and common antigens of Babesia bovis parasites differing in strain and virulence. Journal of Immunology 129, 1700–5.CrossRefGoogle ScholarPubMed
Kuttler, K. L. & Johnson, L. W. (1980). Immunization of cattle with a Babesia bigemina antigen in Freund's complete adjuvant. American Journal of Veterinary Research 41, 536–7.Google ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Langreth, S. G., Jensen, D. J., Reese, R. T. & Trager, W. (1978). Fine structure of human malaria in vitro. Journal of Protozoology 25, 443–52.CrossRefGoogle ScholarPubMed
Mahoney, D. F. & Goodger, B. V. (1981). The isolation of Babesia parasites and their products from the blood. In Babesiosis (ed. Ristic, M. & Kreier, J. P.), pp. 323–335. New York: Academic Press.Google ScholarPubMed
Mahoney, D. F. & Wright, I. G. (1976). Babesia argentina: Immunization of cattle with killed antigen against infection with a heterologous strain. Veterinary Parasitology 2, 273–82.CrossRefGoogle Scholar
Mahoney, D. F., Wright, I. G. & Goodger, B. V. (1981). Bovine babesiosis: The immunization of cattle with fractions of erythrocytes infected with Babesia bovis (Syn B. argentina). Veterinary Immunology and Immunopathology 2, 145–56.CrossRefGoogle ScholarPubMed
McElwain, T. F., Palmer, G. H., Goff, W. L. & Mcguire, T. C. (1988). Identification of Babesia bigemina and B. bovis merozoite proteins with isolate- and species- common epitopes recognized by antibodies in bovine immune sera. Infection and Immunity 56, 1658–60.CrossRefGoogle Scholar
McElwain, T. F., Perryman, L. B., Davis, W. C. & McGuire, T. C. (1987). Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites. Journal of Immunology 138, 2298–304.CrossRefGoogle ScholarPubMed
Morrison, W. I. (1989). Immunological control of ticks and tick-borne parasitic diseases of livestock. Parasitolog 98 (Suppl.), S69–S85.CrossRefGoogle ScholarPubMed
O'Donoghue, P. J., Friedhoff, K. T., Vizcaino, O. G. & Weyreter, H. (1985). The detection of IgM and IgG antibodies against Babesia bigemina in bovine sera using semi-defined antigens in enzyme immunoassays. Veterinary Parasitology 18, 1–12.CrossRefGoogle ScholarPubMed
Palmer, D. A., Buening, G. M. & Carson, C. A. (1982). Cryopreservation of Babesia bovis for in vitro cultivation. Parasitology 84, 567–72.CrossRefGoogle ScholarPubMed
Pirson, P. J. & Perkins, M. E. (1985). Characterization with monoclonal antibodies of a surface antigen of Plasmodium falciparum merozoites. Journal of Immunology 134, 1946–51.CrossRefGoogle ScholarPubMed
Ristic, M. & Montenegro-James, S. (1988). Immunization against Babesia. In Babesiosis of Domestic Animals and Man (ed. Ristic, M.), pp. 163–189. Boca Raton, CRC Press, Inc.Google Scholar
Rodriguez, S. D., Buening, C. M. & Carson, C. A. (1986). Babesia bovis: Purification and concentration of merozoites and infected bovine erythrocytes. Experimental Parasitology 61, 236–43.CrossRefGoogle ScholarPubMed
Rogers, R. J., Dimmock, C. K., De Vos, A. J. & Rodwell, B. J. (1988). Bovine leucosis virus contamination of a vaccine produced in vivo against bovine babesiosis and anaplasmosis. Australian Veterinary Journal 65, 285–7.CrossRefGoogle ScholarPubMed
Smith, R. D. & Ristic, M. (1981). Immunization against bovine babesiosis with culture-derived antigens. In Babesiosis (ed. Ristic, M. & Kreier, J. P.), pp. 485–507. New York: Academic Press.Google ScholarPubMed
Smith, R. D., Carpenter, J., Cabrera, A., Gravely, S. M.Erp, E. E., Osorno, M. & Ristic, M. (1979). Bovine babesiosis: Vaccination against tick-borne challenge exposure with culture-derived Babesia bovis immunogens. American Journal of Veterinary Research 40, 1678–82.Google ScholarPubMed
Snary, D. & Smith, M. A. (1986). An antigenic determinant common to both mouse red blood cells and several membrane proteins of the parasitic protozoa Babesia rodhaini. Molecular and Biochemical Parasitology 20, 101–9.CrossRefGoogle ScholarPubMed
Taylor, S. M., Kenney, J. & Mallons, T. (1983). The effect of multiply rapid passage on strains of Babesia divergens: A comparison of the clinical effects on juvenile and adult cattle of passaged and irradiated parasites. Journal of Comparative Pathology 93, 391–6.CrossRefGoogle Scholar
Vega, C. A., Buening, G. M., Green, T. J. & Carson, C. A. (1985). In vitro cultivation of Babesia bigemina. American Journal of Veterinary Research 46, 416–20.Google ScholarPubMed
Vega, C. A., Buening, G. M., Rodriguez, S. D. & Carson, C. A. (1986). Concentration and enzyme content of in vitro-cultured Babesia bigemina-infected erythrocytes. Journal of Protozoology 33, 514–18.CrossRefGoogle ScholarPubMed
Waltisbuhl, D. J., Goodger, B. V., Wright, I. G., Mirre, G. B. & Commins, M. A. (1987). Babesia bovis: Vaccination studies with three groups of high molecular weight antigens from lysate of infected erythrocytes. Parasitology Research 73, 319–23.CrossRefGoogle ScholarPubMed
Winger, C. M., Canning, E. U. & Culverhouse, J. D. (1987). A monoclonal antibody to Babesia divergens which inhibits merozoite invasion. Parasitology 94, 17–27.CrossRefGoogle ScholarPubMed
Wright, I. C., Goodger, B. V., Leatch, G., Aylward, J. H., Rode-Bramanis, K. & Waltisbuhl, D. J. (1987). Protection of Babesia bigemina-immune animals against subsequent challenge with virulent Babesia bovis. Infection and Immunity 55, 364–8.CrossRefGoogle ScholarPubMed
Wright, I. G., Mirre, G. B., Rode-Bramanis, K., Chamberlain, M., Goodger, B. V. & Waltisbuhl, D. J. (1985). Protective vaccination against virulent Babesia bovis with a low-molecular-weight antigen. Infection and Immunity 48, 109–13.CrossRefGoogle ScholarPubMed
Wright, I. G. & Riddles, P. W. (1986). Biotechnological control of tick-borne disease. Report of the FAO Expert Consultation on Biotechnology for Livestock Production and Health. Rome, Italy, 6–1010 1986.Google Scholar
Wright, I. G., White, M., Tracey-Patte, P. D., Donaldson, R. A., Goodger, B. V., Waltisbuhl, D. J. & Mahoney, D. F. (1983). Babesia bovis: Isolation of a protective antigen by using monoclonal antibodies. Infection and Immunity 41, 244–50.CrossRefGoogle ScholarPubMed