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Identification and sequence comparison of a cuticular collagen of Brugia pahangi

Published online by Cambridge University Press:  06 April 2009

M. Bisoffi  and
B. Betschart
M. Bisoffi
Affiliation:
Department of Medical Parasitology, Swiss Tropical Institute, 4051 Basel, Switzerland
B. Betschart*
Affiliation:
Institute of Zoology, University of Neuchâtel, 2007 Neuchâtel, Switzerland
*
*Corresponding author. University of Neuchâtel, Institute of Zoology, Rue Emile-Argand 11, 2007 Neuchâtel, Switzerland. Tel: + + 41 38 23 30 45. Fax: + + 41 38 23 30 01. E-mail: [email protected].
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Summary

The cuticle of filarial nematodes is a specialized extracellular matrix that covers the parasite and protects it from adverse conditions of the environment. As a surface structure it is in direct contact with the host defence mechanisms and therefore plays an important role in the molecular host-parasite relationship. Using polyclonal antisera raised against the insoluble components of the cuticle of the adult filarial parasite Brugia pahangi, we have isolated cDNA clones encoding collagen molecules of the cuticle. The protein domain structure of cDNA clone Bpcol-1 was compared with the known structures of cuticular collagens of the nematodes Brugia malayi, Caenorhabditis elegans, Ascaris suum and Haemonchus contortus, confirming interspecies similarities. Using affinity-purified anti-Bpcol-1 antibodies we identified Bpcol-1 antigenic determinants in different nematode extracts, and determined the localization of such epitopes within the cuticle of B. pahangi.

Keywords

Brugia pahangifilarial nematodecuticlecollagensequence comparisonimmunolocalization
Type
Research Article
Information
Parasitology , Volume 113 , Issue 2 , August 1996 , pp. 145 - 155
Copyright
Copyright © Cambridge University Press 1996

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