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Identification and in situ and in vitro characterization of secreted proteins produced by plant-parasitic nematodes

Published online by Cambridge University Press:  06 April 2009

R. H. C. Curtis
R. H. C. Curtis
Affiliation:
Entomology and Nematology Department, IACR Rothamsted, Harpenden, Herts AL5 2JQ, UK
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Summary

Secretions of plant-parasitic nematodes which are released into plant tissue may play critical roles in plant-nematode interactions. The identification and characterization of these molecules are of fundamental importance and may help to facilitate the development of novel strategies to interfere with nematode infection of plants and thereby decrease nematode-induced damage to crops. An antibody-based approach was used to isolate molecules present on the nematode surface and in nematode secretions. Monoclonal antibodies (MAbs) were produced to secretions and to whole Heterodera avenue 2nd-stage juveniles; several of these MAbs recognized molecules present in nematode secretions produced in vitro. Three of these molecules have been partly characterized in H. avenae, Globodera rostochiensis, G. pallida and Meloidogyne incognita. A MAb reacting with the surfaces of these nematodes recognized antigens of different molecular weight in each of the species tested. This difference in antigenicity might be related to specific functions in these nematodes. Preliminary results show that this antibody also localized the antigen in root cells surrounding the feeding site induced by M. incognita in Arabidopsis thaliana.

Keywords

plant-parasitic nematodesmonoclonal antibodyimmunolocalizationamphidssurface coatcuticle
Type
Research Article
Information
Parasitology , Volume 113 , Issue 6 , December 1996 , pp. 589 - 597
Copyright
Copyright © Cambridge University Press 1996

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