Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-28T01:07:34.131Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification and diagnosis of Leishmania mexicana complex isolates by polymerase chain reaction

Published online by Cambridge University Press:  06 April 2009

S. Eresh ,
S. M. McCallum  and
D. C. Barker
S. Eresh
Affiliation:
MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
S. M. McCallum
Affiliation:
MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
D. C. Barker
Affiliation:
MRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
Get access Rights & Permissions [Opens in a new window]

Summary

Following cloning of Leishmania (L.) amazonensis kinetoplast DNA two recombinant clones were identified: one specific for L. (L.) amazonensis and the other specific for L. (L.) amazonensis and closely related isolates. DNA sequences from these clones were compared with those of other kinetoplastids and oligonucleotide primers were designed to be used in the polymerase chain reaction. A pair of these primers has been shown not only to be highly specific for L. mexicana complex isolates but can also be used to distinguish between L. (L.) mexicana and L. (L.) amazonensis isolates. These primers have been tested with water-lysed cultures, crude DNA extracts from human patients, potential host reservoirs, sandfly vectors and with cell pellets after isoenzyme characterization. The results of these tests indicate that the primers can be used specifically in the presence of excess host DNA originating from the majority of South American countries.

Keywords

Leishmania mexicana complexpolymerase chain reactionkinetoplast DNAdiagnosis of leishmaniasis
Type
Research Article
Information
Parasitology , Volume 109 , Issue 4 , November 1994 , pp. 423 - 433
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ashford, R. W., Desjeux, P. & Deraadt, P. (1992). Estimation of populations at risk of infection with leishmaniasis. Parasitology Today 8, 104–5.CrossRefGoogle Scholar
Barker, D. C. (1989). Molecular approaches to DNA diagnosis. Parasitology 99, S125–S146.CrossRefGoogle ScholarPubMed
Barker, D. C. & Butcher, J. (1983). The use of DNA probes in the identification of leishmanias: discrimination between isolates of the Leishmania mexicana and L. braziliensis complexes. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 285–97.CrossRefGoogle ScholarPubMed
Barker, D. C., Gibson, L. J., Kennedy, W. P. K., Nasser, A. A. A. A. & Williams, R. H. (1986). The potential of using recombinant DNA species-specific probes for the identification of tropical Leishmania. Parasitology 91, S139–S174.CrossRefGoogle Scholar
Barral, A., Pedral-Sampaio, D., Grimaldi, G. Jr, Momen, H., McMahon-Pratt, D., Ribeiro De Jesus, A., Almeida, R., Badaro, R., Barral-Netto, M., Carvalho, E. M. & Johnson, W. D. Jr (1991). Leishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease. American Journal of Tropical Medicine and Hygiene 44, 536–46.CrossRefGoogle ScholarPubMed
de Bruijn, M. H. L. & Barker, D. C. (1992). Diagnosis of New World leishmaniasis: specific detection of species of the Leishmania braziliensis complex by amplification of kinetoplast DNA. Acta Tropica 52, 45–58.CrossRefGoogle ScholarPubMed
de Bruijn, M. H. L., Labrada, L. A., Smyth, A. J., Santrich, C. & Barker, D. C. (1993). A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis. Tropical Medicine and Parasitology 44, 201–7.Google Scholar
Eperon, S. & McMahon-Pratt, D. (1989). Extracellular amastigote-like forms of Leishmania panamensis and Leishmania braziliensis. Stage and species specific monoclonal antibodies. Journal of Protozoology 36, 510–18.CrossRefGoogle ScholarPubMed
Eresh, S., Mendoza-Leon, A. & Barker, D. C. (1993). A small chromosome of Leishmania (Viannia) braziliensis contains multicopy sequences which are complex specific. Acta Tropica 55, 33–46.CrossRefGoogle ScholarPubMed
Evans, D. (1989). Handbook on isolation, characterisation and cryopreservation of Leishmania, pp. 10–15. UNDP/World Bank/WHO Special Programme for Research and Training for Tropical Diseases (TDR), 1211 Geneva, Switzerland.Google Scholar
Herwaldt, B. L., Arana, B. A. & Navin, T. R. (1992). The natural history of cutaneous leishmaniasis in Guatemala. Journal of Infectious Diseases 165, 518–27.CrossRefGoogle ScholarPubMed
Jackson, P. R., Lawrie, J. M., Stiteler, J. M., Hawkins, D. W., Wohlhieter, J. A. & Rowton, E. D. (1986). Detection and characterisation of Leishmania species and strains from mammals and Vectors by hybridisation and restriction endonuclease digestion of kinetoplast DNA. Veterinary Parasitology 20, 195–215.CrossRefGoogle ScholarPubMed
Kennedy, W. P. K. (1984). Novel identification of differences in the kinetoplast DNA of Leishmania isolates by recombinant DNA techniques and in situ hybridisation. Molecular and Biochemical Parasitology 12, 313–25.Google ScholarPubMed
Lainson, R. & Shaw, J. J. (1987). Evolution, classification and geographical distribution. In The Leishmaniases in Biology and Medicine (ed. Peters, W. & Killick-Kendrick, R.), Vol 1, pp. 1–120. London: Academic Press.Google Scholar
le Blancq, S. M., Lanham, S. M. & Evans, D. A. (1987). Comparative isoenzyme profiles of Old and New World Leishmania. In The Leishmaniases in Biology and Medicine (ed. Peters, W. & Killick-Kendrick, R.), Vol 1, pp. 543–550. London: Academic Press.Google Scholar
Lopez, M., Inga, R., Cangalaya, M., Echevarria, J., Llanos-Cuentas, A., Orrego, C. & Arevalo, J. (1993). Diagnosis of Leishmania using the polymerase chain reaction: a simplified procedure for field work. American Journal of Tropical Medicine and Hygiene 49, 348–56.CrossRefGoogle ScholarPubMed
Macina, R. A., Sanchez, D. O., Gluschankof, D. A., Burrone, O. R. & Frasch, A. C. C. (1986). Sequence diversity in the kinetoplast DNA minicircles of Trypanosoma cruzi. Molecular and Biochemical Parasitology 21, 25–32.CrossRefGoogle ScholarPubMed
Manson-Bahr, P. C. (1987). Diagnosis. In The Leishmaniases in Biology and Medicine (ed. Peters, W. & Killick-Kendrick, R.), Vol 2, pp. 703–729. London: Academic Press.Google Scholar
Marsden, P. D. (1985). Clinical presentations of Leishmania braziliensis braziliensis. Parasitology Today 1, 129–33.CrossRefGoogle ScholarPubMed
Marsden, P. D. (1988). South American Trypanosomiasis and Leishmaniasis: endemic diseases of continental dimensions affecting poor, neglected and underfunded people. In The Biology of Parasitism (ed. Englund, P. T. & Sher, A.), pp. 77–92. New York: Alan R. Liss Inc.Google Scholar
McMahon-Pratt, D. & David, J. R. (1981). Monoclonal antibodies that distinguish between New World species of Leishmania. Nature, London 291, 581–3.CrossRefGoogle Scholar
Messing, J. & Vieira, J. (1982). The pUC plasmids, a M13mp7 derived system for the insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259–68.Google Scholar
Miles, M. A., Povoa, M. M., De Souza, A. A., Lainson, R. & Shaw, J. J. (1980). Some methods for the enzyme characterisation of Latin American Leishmania, with particular reference to Leishmania mexicana amazonensis, and subspecies of Leishmania hertigi. Transactions of the Royal Society of Tropical Medicine and Hygiene 74, 243–52.CrossRefGoogle ScholarPubMed
Ntambi, J. M. & Englund, P. T. (1985). A gap at a unique location in newly replicated kinetoplast DNA minicircles from Trypanosoma equiperdum. Journal of Biological Chemistry 260, 5574–9.CrossRefGoogle Scholar
Pacheco, R. S., Grimaldi, G., Momen, H. & Morel, C. M. (1990). Population heterogeneity among clones of New World Leishmania species. Parasitology 100, 393–8.CrossRefGoogle ScholarPubMed
Ponzi, M., Pace, T. & Frontali, C. (1985). Identification of a telomeric DNA sequence in Plasmodium berghei. EMBO Journal 4, 2991–5.CrossRefGoogle ScholarPubMed
Ramirez, J. L. & Guevara, P. (1987). The ribosomal gene spacer as a tool for the taxonomy of Leishmania. Molecular and Biochemical Parasitology 22, 177–83.CrossRefGoogle ScholarPubMed
Rodgers, M. R., Popper, S. J. & Wirth, D. F. (1990). Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Experimental Parasitology 71, 267–75.CrossRefGoogle ScholarPubMed
Rogers, W. O. & Wirth, D. F. (1987). Kinetoplast DNA minicircles: regions of extensive sequence divergence. Proceedings of the National Academy of Sciences, USA 84, 565–9.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning – A Laboratory Manual, 2nd Edn.Cold Spring Harbor: Cold Spring Harbor Laboratory Press.Google Scholar
Silveira, F. T., Lainson, R., Shaw, J. J. & Ribeiro, R. S. M. (1984). Leishmaniose cutaˇnea na Amazoˇnia. Registro do primeiro caso humano de infecçaˇo mista, determinado por duas espécies distintas de leishmaˇnias: Leishmania braziliensis e Leishmania mexicana amazonensis. Revista do Instituto de Medicina Tropical de São Paulo 26, 272–5.CrossRefGoogle Scholar
Smith, D. F., Searle, S., Ready, P. D., Grammicia, M. & Ben-Ismail, R. (1989). A kinetoplast DNA probe diagnostic for Leishmania major: sequence homologies between regions of Leishmania minicircles. Molecular and Biochemical Parasitology 37, 213–24.CrossRefGoogle ScholarPubMed
Smyth, A. J. (1992). Sequence variation in Leishmania kinetoplast DNA minicircles and diagnosis of leishmaniasis. Ph.D. thesis, University of Cambridge.Google Scholar
Smyth, A. J., de Bruijn, M. H. L., Hassan, M., Basu, D., Mallik, K. K., Adhya, S. & Barker, D. C. (1992). Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kala-azar patients. Parasitology 105, 183–92.CrossRefGoogle ScholarPubMed
World Health Organization (1990). Control of leishmaniases. Report of a WHO Expert Committee, WHO Technical Report Series No. 793. Geneva: World Health Organization.Google Scholar
Wirth, D. F. & McMahon-Pratt, D. (1982). Rapid identification of Leishmania species by specific hybridisation of kinetoplast DNA in cutaneous lesions. Proceedings of the National Academy of Sciences, USA 79, 6999–7003.CrossRefGoogle ScholarPubMed
Wirth, D. F. & Rogers, W. O. (1985). Rapid identification of Leishmania species using specific hybridisation of kinetoplast DNA sequences. In Rapid Detection and Identification of Infectious Agents (ed. Kingsbury, D. T. & Fallow, S.), pp. 127–137. New York: Academic Press.Google Scholar