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Hypoxanthine: a low molecular weight factor essential for growth of erythrocytic Plasmodium falciparum in a serum-free medium

Published online by Cambridge University Press:  06 April 2009

H. Asahi ,
T. Kanazawa ,
Y. Kajihara ,
K. Takahashi  and
T. Takahashi
H. Asahi*
Affiliation:
Department of Parasitology, National Institute of Health, 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162, Japan
T. Kanazawa
Affiliation:
Department of Parasitology, National Institute of Health, 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162, Japan
Y. Kajihara
Affiliation:
Life Tech Division, Nihon Pharmaceutical Co. Ltd, Sumiyoshi-cho 26, Izumisano, Osaka 598, Japan
K. Takahashi
Affiliation:
Life Tech Division, Nihon Pharmaceutical Co. Ltd, Sumiyoshi-cho 26, Izumisano, Osaka 598, Japan
T. Takahashi
Affiliation:
Institute of Medicinal Chemistry, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142, Japan
*
*Corresponding author. Tel: +3 5285 1111. Fax: +3 5285 1150.
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Summary

A low molecular weight factor in a basal medium essential for erythrocytic Plasmodium falciparum development in a serum-free medium using a cell growth-promoting factor derived from adult bovine serum was detected. The factor was hypoxanthine. The optimal hypoxanthine concentration for parasite growth was between 15 and 120 μM. The contribution of hypoxanthine to increased parasite growth was clearly evident in cultures on day 4. Among various low molecular weight supplements tested, adenine, adenosine, AMP, ATP, cyclic AMP, guanine, guanosine, inosine, inosine mono-phosphate, xanthine, NAD, NADH, NADP, NADPH and deoxyguanosine triphosphate showed a similar effect to that of hypoxanthine in the serum-free culture system. On the other hand, the addition of uric acid, FAD, thymidine, uridine, orotic acid, deoxythymidine triphosphate, deoxycytidine triphosphate, deoxyadenosine triphosphate, ribose-1-phosphate, or ethanolamine was not beneficial to the parasite growth. The results presented here will not only be of practical value, but will provide important information about the developmental requirements of the parasite.

Keywords

Plasmodium falciparumhuman malariaserum-free cultureessential low molecular weight factor
Type
Research Article
Information
Parasitology , Volume 113 , Issue 1 , July 1996 , pp. 19 - 23
Copyright
Copyright © Cambridge University Press 1996

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