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Haemogregarines from western Palaearctic freshwater turtles (genera Emys, Mauremys) are conspecific with Haemogregarina stepanowi Danilewsky, 1885

Published online by Cambridge University Press:  26 November 2013

NELA DVOŘÁKOVÁ ,
JANA KVIČEROVÁ ,
IVO PAPOUŠEK ,
HOSSEIN JAVANBAKHT ,
GHOULEM TIAR ,
HAJIGHOLI KAMI  and
PAVEL ŠIROKÝ
NELA DVOŘÁKOVÁ
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic
JANA KVIČEROVÁ
Affiliation:
Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic Department of Parasitology, Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
IVO PAPOUŠEK
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic
HOSSEIN JAVANBAKHT
Affiliation:
Department of Biology, Razi University, Baghabrisham 67149, Kermanshah, Iran
GHOULEM TIAR
Affiliation:
Department of Biology, University of Badji Mokhtar, BP 12, El Hadjar, 23000 Annaba, Algeria
HAJIGHOLI KAMI
Affiliation:
Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
PAVEL ŠIROKÝ*
Affiliation:
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic
*
* Corresponding author: Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic. E-mail: [email protected]
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Summary

The majority of Haemogregarina species have been based on the morphology of their erythrocytic stages and supposed strict host specificity. The quantity of species with a limited number of overlapping diagnostic traits has led to a considerable mess in haemogregarine taxonomy and significant synonymy. We analysed host specificity, intra- and interspecific variability, evolutionary relationships, and the distribution of the type species of the genus Haemogregarina – H. stepanowi. The morphology of blood stages and 18S rDNA sequences of this haemogregarine from four western Palaearctic hard-shelled freshwater turtles (Emys orbicularis, Mauremys caspica, Mauremys leprosa and Mauremys rivulata) were compared with Haemogregarina balli. Additional sequences of 18S rDNA of Haemogregarina-like isolates collected from three species of African hinged terrapins (genus Pelusios) were used to enlarge the dataset for phylogenetic analyses. Thirteen sequences (1085 bp) of Haemogregarina representing all four western Palaearctic turtle species were identical, corresponding to H. stepanowi, which is closely related to the Nearctic species H. balli. In our analyses, Haemogregarina spp. constituted a monophyletic clade sister to the genus Hepatozoon. Haemogregarina stepanowi possesses a wide distribution range from the Maghreb, through Europe, Turkey and the Middle East to Iran. We consider that the genus Haemogregarina has a low host specificity crossing the family level of its vertebrate hosts and that its distribution is likely to be linked to the vector and definitive host – the leech.

Keywords

ApicomplexaHaemogregarinaEmysMauremysPelusios18S rDNAphylogenetic analysishost specificity
Type
Research Article
Information
Parasitology , Volume 141 , Issue 4 , April 2014 , pp. 522 - 530
Copyright
Copyright © Cambridge University Press 2013 

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