Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-20T00:37:25.850Z Has data issue: false hasContentIssue false
  • English
  • Français

Giardia infection in pigs: detection and in vitro isolation of trophozoites of the Giardia intestinalis group

Published online by Cambridge University Press:  06 April 2009

B. Koudela ,
E. Nohýnková ,
J. Vítovec ,
M. Pakandl  and
J. Kulda
B. Koudela
Affiliation:
Department of Protozooses of Farm Animals, Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, Ceské Budějovice 370 05, Czechoslovakia
E. Nohýnková
Affiliation:
Laboratory of Protozoology, Institute of Tropical Medicine, Sokolská 49, Prague 2, 120 00, Czechoslovakia
J. Vítovec
Affiliation:
Department of Protozooses of Farm Animals, Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, Ceské Budějovice 370 05, Czechoslovakia
M. Pakandl
Affiliation:
Department of Protozooses of Farm Animals, Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, Ceské Budějovice 370 05, Czechoslovakia
J. Kulda
Affiliation:
Department of Parasitology, Faculty of Science, Charles University, Viničná 7, Prague 2, 128 44, Czechoslovakia
Get access Rights & Permissions [Opens in a new window]

Summary

Giardia infections were detected in 14 out of 32 weaned, 1 to 2-month-old pigs, used in experiments with coccidia. Giardia trophozoites were present in the small intestine from the duodenum to the ileum with maximum numbers in the cranial part of the upper jejunum. They were localized mainly on the surface of intestinal crypts. No histological changes accompanied the infection and the infected animals were asymptomatic. Giardia trophozoites obtained from the intestines of necropsied pigs were isolated in axenic culture in a bile-supplemented TYI-S-33 medium. Morphological features of the trophozoites revealed by both light and scanning electron microscopy proved that the organism belongs to the Giardia intestinalis group.

Keywords

Giardia intestinalispigtrophozoite morphologyscanning electron microscopyaxenic culture
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 163 - 166
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Davies, R. B. & Hibler, C. P. (1979). Animal reservoirs and cross-species transmission of giardiasis. In Waterborne Transmission of Giardiassis (ed. Jakubowski, W. & Hoff, J. C.), pp. 104–26. Cincinnati, Ohio: Environmental Protection Agency.Google Scholar
Erlandsen, S. L., Bemrick, W. J. & Pawley, J. (1989). High-resolution electron microscopic evidence for the filamentous structure of the cyst wall in Giardia muris and Giardia duodenalis. Journal of Parasitology 75, 787–97.Google Scholar
Faubert, G. M. (1988). Evidence that giardiasis is a zoonosis. Parasitology Today 4, 66–8.CrossRefGoogle ScholarPubMed
Frye, W. W. & Meleney, H. E. (1932). Investigations of Entamoeba histolytica and other intestinal protozoa in Tennessee. IV. A study of flies, rats, mice and some domestic animals as possible carriers of the intestinal protozoa of man in a rural community. American Journal of Hygiene 16, 729–49.Google Scholar
Hewlett, E. L., Andrews, J. S., Ruffier, J. & Schafter, F. W. (1982). Experimental infection of mongrel dogs with Giardia lamblia cysts and cultured trophozoites. Journal of Infectious Diseases 152, 1166–71.Google Scholar
Keister, D. B. (1983). Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 487–8.CrossRefGoogle ScholarPubMed
Kirkpatrick, C. E. (1989). Giardiasis in large animals. Compendium in Continuing Education for the Practicing Veterinarian 11, 80–4.Google Scholar
Kirkpatrick, C. E. & Green, B. (1980). Susceptibility of domestic cats to infections with Giardia lamblia cysts and trophozoites from human sources. Journal of Clinical Microbiology 21, 678–80.CrossRefGoogle Scholar
Levine, N. D. (1973). Protozoan Parasites of Domestic Animals and of Man. 2nd Edn.Minneapolis: Burgess Publishing Company.Google Scholar
Mirzayans, P. (1976). Parasitic diseases of pigs in Iran. In Proceedings of the 4th International Pig Veterinary Society Congress, Ames, Iowa, USA, June 22–24, 1976, p. 62. Ames: International Pig Veterinary Society.Google Scholar
Taminelli, V., Eckert, J., Sydler, T., Gottstein, B., Corboz, L. & Hofmann, M. (1989). Experimental infection of calves and lambs with bovine Giardia isolates. Schweizerisches Archiv für Tierheilkunde 131, 551–64.Google Scholar
Thomson, R. C. A., Lymbery, A. J. & Meloni, B. P. (1990). Genetic variation in Giardia Kunstler, 1882: taxonomic and epidemiological significance. Protozoological Abstracts 14, 1–28.Google Scholar
Yang, J. J. (1975). Survey for intestinal parasites of pigs in Korea. Korean Journal of Veterinary Research 15, 309–14.Google Scholar