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Genetically-determined influences on the ability of poor responder mice to respond to immunization against Trichuris muris

Published online by Cambridge University Press:  06 April 2009

K. J. Else  and
D. Wakelin
K. J. Else
Affiliation:
MRC Experimental Parasitology Unit, Department of Zoology, Nottingham University, University Park, Nottingham NG7 2RD
D. Wakelin
Affiliation:
MRC Experimental Parasitology Unit, Department of Zoology, Nottingham University, University Park, Nottingham NG7 2RD
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Summary

Strains of mice poorly (B10) or non-responsive (B10.BR) to a primary infection with Trichuris muris were protected against infection by vaccination with excretory/secretory (E/S) antigen in Complete Freund's Adjuvant (CFA). Protection in these mice was slow to be expressed compared to that in good responder strains. Vaccination boosted the IgG and IgG1 antibody responses to E/S antigen and altered the antigen recognition profiles, three high molecular weight antigens (80–85, 90–95, 105–110 kDa) being recognized by antibodies in sera from vaccinated but not control mice. B10. BR mice which had experienced a patent primary infection could not be protected against challenge infections by vaccination and this was correlated with depressed levels of IgG1, but not total IgG, to E'S antigen early post-challenge compared with vaccinated infected mice which had not seen an adult primary infection. There was also lack of recognition of the three high molecular weight antigens recognized by antibodies in sera from mice infected after vaccination. It is suggested that the rapid development of high levels of IgG1 antibodies, and the recognition of the three high molecular weight antigens, may reflect events that are important in protective immunity. Immunomodulation of host immunity by T. muris may therefore be achieved, at least in part, by the suppression of specific IgG1 levels, the production of an irrelevant IgG isotype and prevention of the recognition of critical antigens.

Keywords

immunizationvaccinationgeneticsimmunomodulationantibody response
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 479 - 489
Copyright
Copyright © Cambridge University Press 1990

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