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Genetic structure of Anisakis physeteris, and its differentiation from the Anisakis simplex complex (Ascaridida: Anisakidae)

Published online by Cambridge University Press:  06 April 2009

S. Mattiucci ,
G. Nascetti ,
L. Bullini ,
P. Orecchia  and
L. Paggi
S. Mattiucci
Affiliation:
Institute of Parasitology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy
G. Nascetti
Affiliation:
Department of Genetics and Molecular Biology, University of Rome ‘La Sapienza’, Via Lancisi 29, 00161 Rome, Italy
L. Bullini
Affiliation:
Department of Genetics and Molecular Biology, University of Rome ‘La Sapienza’, Via Lancisi 29, 00161 Rome, Italy
P. Orecchia
Affiliation:
Institute of Parasitology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy
L. Paggi
Affiliation:
Institute of Parasitology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy
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Summary

The genetic structure of Anisakis physeteris from the Mediterranean Sea has been analysed electrophoretically at 22 enzyme loci. The samples studied, although differing in the life-stage (larvae and adults), and in the host (the fishes Micromesistius poutassou and Trachurus trachurus, and the sperm whale Physeter macrocephalus) were genetically homogeneous. Of these loci 11 (Ldh, Sod, Np, Adk-2, Pgm-1, Est-1, Est-2, Acph-1, Acph-2, Lap-2 and Ca) were found to be monomorphic, while the other 11 (Sdh, Mdh, ldh, 6-Pgdh, G3pdh, Got, Adk-1, Pgm-2, Lap-1, Mpi and Gpi) showed from 2 to 7 alleles. The following values of genetic variability were estimated: He = 0·ll, P = 0·50, A = 1·95. Distinct alleles were found between A. physeteris and the A. simplex complex at 19 out of the 22 loci studied, and only few rare alleles were shared at the remaining 3 loci. The genetic divergence between A. physeteris and A. simplex A and B is therefore very high, the values of Nei’s index D being 7·384 and 6·443 respectively (I = 0·001 and 0·002). The assignation of A. physeteris and the A. simplex complex to two distinct subgenera, Skrjabinisakis and Anisakis, as proposed by Mosgovoy on a morphological basis, appears to be fully justified according to our genetic data.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 383 - 387
Copyright
Copyright © Cambridge University Press 1986

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References

Ayala, F. J., Powell, J. R., Tracey, M. L., Mourao, C. A. & Pérez Salas, S. (1972). Enzyme variability in the Drosophila willistoni group. IV. Genie variation in natural populations of Drosophila willistoni. Genetics 70, 113–39.CrossRefGoogle Scholar
Baylis, H. A. (1923). An ascarid from the sperm whale. Annals and Magazine of Natural History 11, 211–17.CrossRefGoogle Scholar
Berland. B. (1961). Nematodes from some Norwegian marine fishes. Sarsia 2, 1–50.CrossRefGoogle Scholar
Brewer, G. J. & Sing, C. F. (1970). An Introduction to Isozyme Techniques. New York and London: Academic Press.Google Scholar
Bullini, L., Nascetti, G., Ciafre', S., Rumore, F. & Biocca, E. (1978). Ricerche cariologiche ed elettroforetiche su Parascaris univalens c Parascaris equorum. Accademia Nazionale dei Lincei, Rendiconti della Classe di Scienze Fisiche Matematiche e Naturali 65, 151–6.Google Scholar
Bullini, L., Nascetti, G. & Grappelli, C. (1981). Nuovi dati sulla divergenza e sulla variabilità genetica delle specie gemelle Ascaris lumbricoides – Ascaris suum e Parascaris univalens – Parascaris equorum. Parassitologia 23, 139–42.Google Scholar
Bullini, L., Nascetti, G., Paggi, L., Orecchia, P., Mattiucci, S. & Berland, B. (1986). Genetic variation of Ascaridoid worms with different life cycle. Evolution 40, 437–40.CrossRefGoogle Scholar
Harris, H. (1966). Enzyme polymorphism in man. Proceedings of the Royal Society of London, B 169, 298–310.Google Scholar
Harris, H. & Hopkinson, D. A. (1976). Handbook of Enzyme Electrophoresis in Human Genetics. New York: North-Holland.Google Scholar
Mosgovoy, A. A. (1951). Ascarids of mammals of the U.S.S.R. (Anisakoidea). Trudy Gel'mintologicheskoi Laboratorii 5, 12–22. (In Russian.)Google Scholar
Mosgovoy, A. A. (1953). Ascaridata of animals and man, and the diseases provoked by them. Osnovy Nematodologii 2, 8–42. (In Russian.)Google Scholar
Nascetti, G., Grappelli, C. & Bullini, L. (1979). Ricerche sul differenziamento genetico di Ascaris lumbricoides e Ascaris suum. Accademia Nazionale dei Lincei, Rendiconti della Classe di Scienze Fisiche Matematiche e Naturali 67, 457–65.Google Scholar
Nascetti, G., Orecchia, P., Paggi, L., Cagnolati, V., Grappelli, C. & Bullini, L. (1981 a). Ricerche sul differenziamento genetico dei generi Toxocara e Neoascaris. Parassitologia 23, 206–7.Google Scholar
Nascetti, G., Paggi, L., Orecchia, P., Mattiucci, S. & Bullini, L. (1981 b). Divergenza genetica in popolazioni del genere Anisakis del Mediterraneo. Parassitologia 23, 208–10.Google Scholar
Nascetti, G., Paggi, L., Orecchia, P., Mattiucci, S. & Bullini, L. (1983). Two sibling species within Anisakis simplex (Ascaridida: Anisakidae). Parassitologia 25, 306–7.Google Scholar
Nascetti, G., Paggi, L., Orecchia, P., Smith, J. W., Mattiucci, S. & Bullini, L. (1986). Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and North East Atlantic. International Journal for Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Nei, M. (1972). Genetic distance between populations. American Naturalist 106, 238–92.CrossRefGoogle Scholar
Paggi, L., Nascetti, G., Orecchia, P., Mattiucci, S. & Bullini, L. (1985). Biochemical taxonomy and genetic variability of Ascaridoid nematodes. Parassitologia 27, (in the Press).Google Scholar
Paggi, L., Orecchia, P., Bullini, L., Nascetti, G. & Mattiucci, S. (1983). Electrophoretic identification of Anisakis larvae from Mediterranean and North Atlantic. Parassitologia 25, 315–16.Google Scholar
Poulik, M. D. (1957). Starch gel electrophoresis in a discontinuous system of buffers. Nature, London 180, 1477.CrossRefGoogle Scholar
Rudolphi, C. A. (1809). Entozoorum sive vermium intestinalium historia naturalis. 2. Amsterdami.Google Scholar
Selander, R. K., Smith, H. H., Yang, S. Y., Johnson, W. E. & Gentry, J. B. (1971). Biochemical polymorphisms in the genus Peromyscus. I. Variation of the old-field mouse (Peromyscus polionotus). Studies in Genetics 6, University of Texas Publications No. 7103, 49–90.Google Scholar
Shaw, C. R. & Prasad, R. (1970). Starch gel electrophoresis of enzymes: a compilation of recipes. Biochemical Genetics 54, 297–320.CrossRefGoogle Scholar