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Genetic manipulation of schistosomes – progress with integration competent vectors

Published online by Cambridge University Press:  19 August 2011

SUTAS SUTTIPRAPA ,
GABRIEL RINALDI  and
PAUL J. BRINDLEY
SUTAS SUTTIPRAPA
Affiliation:
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037USA
GABRIEL RINALDI
Affiliation:
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037USA Departamento de Genética, Facultad de Medicina, Universidad de la República, (UDELAR), Montevideo 11800, Uruguay
PAUL J. BRINDLEY*
Affiliation:
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037USA
*
*Corresponding author: Paul J. Brindley, Department of Microbiology, Immunology & Tropical Medicine, George Washington University Medical Center, Ross Hall, 2300 I Street, NW, Washington, DC 20037USA. Fax: +1 202 994 2913. E-mail: [email protected]
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Summary

Draft genome sequences for Schistosoma japonicum and S. mansoni are now available. The schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. Nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. Over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. A brief history of genetic manipulation is presented, along with a review of the field with emphasis on reports of integration of transgenes into schistosome chromosomes.

Keywords

Schistosomesgenetic manipulationtransgenesischromosome integrationgerm lineretrovirusmurine leukaemia viruspseudotyped gammaretrovirustransposonpiggyBac
Type
Research Article
Information
Parasitology , Volume 139 , Special Issue 5: Genetic manipulation of parasitic helminths to define gene function , April 2012 , pp. 641 - 650
Copyright
Copyright © Cambridge University Press 2011

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