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Functional characterization of X-prolyl aminopeptidase from Toxoplasma gondii

Published online by Cambridge University Press:  25 May 2016

MINGFA YANG ,
JUN ZHENG ,
HONGLIN JIA  and
MINGXIN SONG
MINGFA YANG
Affiliation:
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China Harbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China
JUN ZHENG
Affiliation:
Harbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China
HONGLIN JIA*
Affiliation:
Harbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China
MINGXIN SONG*
Affiliation:
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China
*
*Corresponding authors: College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin 150001, People's Republic of China. Tel: +8613059000733. E-mail: [email protected] and Harbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Maduan Street 427, Nangang District, Harbin 150001, People's Republic of China. Tel: +8618946066070. E-mail: [email protected]
*Corresponding authors: College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin 150001, People's Republic of China. Tel: +8613059000733. E-mail: [email protected] and Harbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Maduan Street 427, Nangang District, Harbin 150001, People's Republic of China. Tel: +8618946066070. E-mail: [email protected]
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Summary

In the present study, a recombinant aminopeptidase P (rTgAPP) from Toxoplasma gondii was expressed in Escherichia coli to evaluate its enzyme parameters. The rTgAPP showed strong activity against a synthetic substrate for aminopeptidase P at pH 8·0 with a Km value of 0·255 µm and a kcat value of 35·6 s−1. The overall catalytic efficiency (kcat/Km) of the rTgAPP was 139·6 × 105 M−1 s−1. The activity of rTgAPP was enhanced by the addition of divalent cations and inhibited by bestatin. Deletion of TgAPP gene in the parasite through a CRISPR/Cas9 system resulted in inhibition of growth indicating the importance of TgAPP. Thus our findings reveal that TgAPP is an active enzyme in T. gondii and provide an insight into the function of TgAPP.

Keywords

Toxoplasma gondiiToxoplasmosisX-prolyl aminopeptidaseenzymatic activitygrowth
Type
Research Article
Information
Parasitology , Volume 143 , Issue 11 , September 2016 , pp. 1443 - 1449
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Copyright
Copyright © Cambridge University Press 2016 

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