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Fixation and Staining of Trypanosoma lewisi

Published online by Cambridge University Press:  06 April 2009

N. H. Swellengrebel
N. H. Swellengrebel
Affiliation:
(From the Hygienic Inst., University of Amsterdam. Director: Prof. Dr R. H. Saltet.)
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Extract

Since the publication of Moore and Breinl's paper (1908) a new method of wet fixation has been introduced into the technique of protozoological research. It is generally stated that the old method of drying the blood films and fixing them afterwards in absolute alcohol, destroys the minute details of nuclear and protoplasmatic structure; that the preparations made in this way are wholly misleading and the structures do not correspond to those of the living Trypanosomes. This becomes evident when we compare the figures of Moore and Breinl and of Rosenbusch (1909), who studied Tr. lewisi with the aid of wet fixation in Flemming's liquid, with those of other authors (Prowazek (1905), Wenyon (1908) etc.) who studied the same subject. The structure of the nucleus is very dissimilar with the two methods. The big karyosome observed by the first authors cannot be found with dry fixation; then only one or more minute granules are to be seen.

Type
Research Article
Information
Parasitology , Volume 3 , Issue 2 , July 1910 , pp. 226 - 238
Copyright
Copyright © Cambridge University Press 1910

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References

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