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Fine structure of Metchnikovella incurvata Caullery and Mesnil 1914 (microsporidia), a hyperparasite of gregarines Polyrhabdina sp. from the polychaete Pygospio elegans

Published online by Cambridge University Press:  28 February 2013

Y. Y. SOKOLOVA ,
G. G. PASKEROVA ,
Y. M. ROTARI ,
E. S. NASSONOVA  and
A. V. SMIRNOV
Y. Y. SOKOLOVA*
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA
G. G. PASKEROVA
Affiliation:
Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia
Y. M. ROTARI
Affiliation:
Institute of Ecology of the Volga River Basin RAS, Togliatti, Russia
E. S. NASSONOVA
Affiliation:
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia
A. V. SMIRNOV
Affiliation:
Department of Invertebrate Zoology, St. Petersburg State University, St. Petersburg, Russia
*
*Corresponding author. Microscopy Center, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA. Fax: 225 578 9899. E-mail: [email protected]
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Summary

Class Rudimicrosporea Sprague 1977, with its single family Metchnikovellidae, comprises hyperparasites of gregarines from the guts of marine invertebrates. Metchnikovellids remain poorly studied in spite of their significance to the evolutionary history of microsporidia; their ultrastructure and life cycles require further investigation. Here we present results of the light- and electron-microscopy study of Metchnikovella incurvata Caulleri and Mesnil 1914, isolated from lecudinid gregarines, parasitizing polychaetes Pygospio elegans in the White Sea littoral zone, and yet described only on the light-microscopic level. The life cycle of this microsporidium includes 2 sporogonies: free (FS) and sac-bound (SBS). In FS, sporonts develop into multinuclear cells (sporogonial plasmodia), which generate sporoblasts and free spores residing in direct contact with the host cytoplasm. Electron microscopy revealed their metchnikovellidean structure: a horseshoe-shaped nucleus, short manubrium perpendicular to the long axis of the spore, and a polar cap in a separate membrane container. Merogony was not observed. The earliest stages of SBS were chains of binucleate cells. They underwent a series of nuclear and cell divisions, produced extracellular envelopes, and split into boomerang-shaped spore sacs, containing up to 16 spores each. Ultrastructure and sizes of sac-bounded spores were similar to those of free-living ones. An amended diagnosis of M. incurvata is provided.

Keywords

MicrosporidiaMetchnikovellidaparasitologypolychaetesgregarinesultrastructurelife cycle
Type
Research Article
Information
Parasitology , Volume 140 , Issue 7 , June 2013 , pp. 855 - 867
Copyright
Copyright © Cambridge University Press 2013 

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