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Fasciola hepatica: a technique for the study of gut penetration by juvenile flukes

Published online by Cambridge University Press:  08 November 2011

D. J. Burden ,
A. P. Bland ,
D. L. Hughes  and
N. C. Hammet
D. J. Burden
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire
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Summary

A method using light and electron microscopes is described which is suitable for the examination of gut penetration by juvenile Fasciola hepatica. It involved the ligation of small sections of the small intestine of rats and the introduction of artificially excysted flukes into these gut loops. By restricting the area of infection in this way it was possible to either recover flukes from the gut lumen or to prepare ultrathin sections for electron microscopy of flukes penetrating the gut wall. In addition, flukes were recovered from the body cavity at various times after preparation of loops in resistant and naive rats. It was found that more flukes reached the body cavity in naive rats than in resistant rats, demonstrating a resistance to infection in the gut loops of sensitized rats.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 2 , October 1981 , pp. 249 - 252
Copyright
Copyright © Cambridge University Press 1981

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References

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