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The extraction, characterization and in vitro translation of RNA from adult Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

M. P. R. Tenniswood  and
A. J. G. Simpson
M. P. R. Tenniswood
Affiliation:
Laboratory of Developmental Biochemistry, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7
A. J. G. Simpson
Affiliation:
Laboratory of Developmental Biochemistry, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7
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Summary

We have extracted RNA from Schistosoma mansoni using the lithium chloride–urea method which gives good yields of undegraded RNA. The results of agarose gel electrophoresis of RNA extracted by this procedure suggest that S. mansoni has an in vivo nick in the large rRNA sub-unit. Translation of the RNA in a rabbit reticulocyte lysate gave significant incorporation of [35S]methionine into synthesized proteins. Immuno-precipitation of these translation products using a hyperimmune monkey serum sedimented between 5 and 8% of the radioactivity, which appeared to be present in approximately 13 proteins of molecular weights 18, 20, 21, 22, 23, 35, 40, 54, 60, 70, 74, 78 and 105 K Daltons.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 2 , April 1982 , pp. 253 - 261
Copyright
Copyright © Cambridge University Press 1982

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