Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-23T12:00:18.111Z Has data issue: false hasContentIssue false

Expression of NF-Y nuclear factor in Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

E. Serra
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
J.-L. Liu
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
A. Capron
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
K. Zemzoumi
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
C. Dissous*
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
*
*Corresponding author. Tel: + 33 20 877350. Fax: + 33 20 877888.

Summary

The A subunit of NF-Y nuclear factor from Schistosoma mansoni was expressed in E. coli fused to a histidine tag and purified by affinity chromatography using a Ni2+-Agarose matrix. Antibodies against the recombinant protein were prepared and used for Western blot and immunolocalization. The presence of SMNF-YA in all stages of the parasite life-cycle was determined by RT-PCR and Western blot analysis. The immunolocalization of SMNF-YA showed the presence of this factor in a parenchymal cell population of cercariae and adult worms and in embryos within eggs. The expression of SMNF-YA was demonstrated to decrease in maturating spermatozoites whereas an accumulation of this factor was observed in the nucleus from oocytes during their maturation processes.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Benoist, C. & Mathis, D. (1990). Regulation of major histocompatibility complex class II genes: X, Y and other letters of the alphabet. Annual Review in Immunology 8, 681715.CrossRefGoogle Scholar
Bucher, P. (1990). Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences. Journal of Molecular Biology 212, 563578.CrossRefGoogle ScholarPubMed
Chodosh, L., Baldwin, A., Carthew, R. & Sharp, P. (1988). Human CCAAT binding proteins have heterologous subunits. Cell 53, 1124.CrossRefGoogle ScholarPubMed
Danilition, S., Frederickson, R., Taylor, C. & Miyamoto, N. (1990). Transcription factor binding and spacing constraints in the human β-actin proximal promoter. Nucleic Acids Research 19, 69136922.Google Scholar
Diamond, M., Miner, J., Yoshinaga, S. & Yamamoto, K. (1990). Transcription factor interactions: selectors of positive or negative regulation from a single DNA element. Science 249, 12661272.Google Scholar
Dorsey, C. H. & Stirewalt, M. A. (1971). Schistosoma mansoni: Fine structure of cercarial acetabular glands. Experimental Parasitology 30, 199214.CrossRefGoogle ScholarPubMed
Hatamochi, A., Golumbek, P., Van Shaftingen, E. & De Combrugghe, B. (1988). A CCAAT DNA binding factor consisting of two different components that are both required for DNA binding. Journal of Biological Chemistry 263, 59405947.CrossRefGoogle ScholarPubMed
Henkle, K., Cook, C., Foster, L., Engman, D., Bobek, L., Cain, G. & Domeldson, J. (1990). The gene family encoding eggshell proteins of Schistosoma mansoni. Molecular and Biochemical Parasitology 42, 6982.CrossRefGoogle Scholar
Kaplan, B., Bernstein, S. & Gioio, A. (1979). An improved method for the rapid isolation of brain ribonucleic acid. The Biochemical Journal 185, 181184.CrossRefGoogle Scholar
Khalife, J., Godin, C. & Capron, A. (1995). Transcriptional regulation of Schistosoma mansoni calreticulin: possible role of AP-1. Parasitology 111, 469475.CrossRefGoogle ScholarPubMed
Khalife, J., Pierce, R., Godin, C. & Capron, A. (1993). Cloning and sequencing of the gene encoding Schistosoma mansoni calreticulin. Molecular and Biochemical Parasitology 62, 313316.Google Scholar
Kim, C. & Sheffery, M. (1990). Physical characterization of the purified CCAAT transcription factor, αCP-1. Journal of Biological Chemistry 265, 1336213369.Google Scholar
Landsberger, N. & Wolffe, A. (1995). Role of chromatin and Xenopus laevis heat shock transcription factor in regulation of transcription from the X. laevis hsp promoter in vivo. Molecular and Cellular Biology 15, 60136024.CrossRefGoogle Scholar
Levy-Holtzman, R. & Schechter, I. (1995). Activity of the promoter of hsp70 gene of the parasite helminth Schistosoma mansoni in the mammalian CHO cell-line. Biochimica et Biophysica Acta 1263, 9698.Google Scholar
Li, X., Mantovani, R., Hooft Van Huijsduijnen, R., Andre, I., Benoist, C. & Mathis, D. (1992). Evolutionary variation of the CCAAT binding transcription factor NF-Y. Nucleic Acids Research 20, 10871091.CrossRefGoogle ScholarPubMed
McKnight, S & Tjian, R. (1986). Transcription selectivity of viral genes in mammalian cells. Cell 46, 795805.CrossRefGoogle ScholarPubMed
McNair, A., Dissous, C., Duvaux-Miret, O. & Capron, A. (1993). Cloning and characterization of the gene encoding the 28 kDa glutathione S-transferase of Schistosoma mansoni. Gene 124, 245249.CrossRefGoogle ScholarPubMed
Mitchell, P. & Tjian, R. (1989). Transcription regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245, 371378.CrossRefGoogle ScholarPubMed
Morgan, W., Williams, G., Morimoto, R., Greene, J., Kingston, R. & Tjian, R. (1987). Two transcriptional activators, CCAAT-box-binding transcription factor and heat shock transcription factor, interact with a human hsp70 gene promoter. Molecular and Cellular Biology, 7 11291138.Google ScholarPubMed
Neumann, S., Ziv, E., Lanter, F. & Schechter, I. (1992). Cloning and sequencing of an hsp70 gene from Schistosoma mansoni. Molecular and Biochemical Parasitology 56, 357360.CrossRefGoogle ScholarPubMed
Olesen, J., Fikes, J. & Guarente, L. (1987). The Schizosaccharomyces pombe homologue of Saccharomyces cerevisiae HAP2 reveals selective and stringent conservation of the small essential core protein domain. Molecular and Cellular Biology 11, 611619.Google Scholar
Raymondjean, M., Cereghini, S. & Yaniv, M. (1988). Several distinct ‘CCAAT’ box binding proteins coexist in eukaryotic cells. Proceedings of the National Academy of Sciences, USA 85, 757761.CrossRefGoogle ScholarPubMed
Reith, W., Siegrist, C.-A., Barras, E. & Mach, B. (1994). Function of major histocompatibility complex class II promoters requires cooperative binding between factors RFX and NF-Y. Proceedings of the National Academy of Sciences, USA 91, 554558.CrossRefGoogle ScholarPubMed
Ronchi, A., Bellorini, M., Mongelli, N. & Mantovani, R. (1995). CCAAT-box binding protein NF-Y (CBF.CP1) recognizes the minor groove and distors DNA. Nucleic Acids Research 23, 45654572.Google Scholar
Sambrook, J., Fritsch, E. & Maniatis, L. (1989). Molecular Cloning: A Laboratory Manual. 2nd Edn.Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.Google Scholar
Szabo, S., Gold, J., Murphy, T. & Murphy, K. (1993).Identification of a cis-acting regulatory element controlling interleukin 4 gene expression in T cells: roles of NF-Y and NF ATc. Molecular and Cellular Biology 13, 47934805.Google Scholar
Wright, K., Moore, T., Vilen, B., Brown, A. & Ting, J. (1995). Major histocompatibility complex class II-associated invariant chain gene expression is up-regulated by cooperative interactions of Sp1 and NF-Y. Journal of Biological Chemistry 270, 2097820986.CrossRefGoogle ScholarPubMed
Zelesnik-Le, N., Azizkhan, J. & Ting, J. (1991). Affinity purified CCAAT box binding protein (YEBP) functionally regulates expression of human class II major histocompatibility complex gene and herpes simplex virus thymidine kinase gene. Proceedings of the National Academy of Sciences, USA 88, 18731877.CrossRefGoogle Scholar
Zemzoumi, K., Dissous, C., Cochu, A., Trolet, J., Capron, A. & McNair, A. (1995). Schistosoma mansoni: interaction of nuclear extracts with the CCAAT binding site revealed by gel shift assay. Experimental Parasitology 80, 149154.CrossRefGoogle ScholarPubMed
Zemzoumi, K., Serra, E., Mantovani, R., Trolet, J., Capron, C. & Dissous, C. (1996). Cloning of Schistosoma mansoni transcription factor NF-YA subunit: phylogenic conservation of the HAP-2 homology domain. Molecular and Biochemical Parasitology 77, 161172.CrossRefGoogle ScholarPubMed