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Experimental Schistosoma bovis infection in goats. Circulating antigen and antibody responses to egg and adult worm antigens during infection and following treatment with praziquantel

Published online by Cambridge University Press:  06 April 2009

M. V. Johansen ,
Y. Fillié ,
J. Monrad ,
N. Ø. Christensen  and
A. Deelder
M. V. Johansen*
Affiliation:
Danish Bilharziasis Laboratory, Jægersborg Allé 1D, DK-2920 Charlottenlund, Denmark
Y. Fillié
Affiliation:
Laboratory of Parasitology, Medical Faculty, University of Leiden, Wassenaarseweg 62, P.O. Box 9605, 2300 RC Leiden, The Netherlands
J. Monrad
Affiliation:
Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark
N. Ø. Christensen
Affiliation:
Danish Bilharziasis Laboratory, Jægersborg Allé 1D, DK-2920 Charlottenlund, Denmark
A. Deelder
Affiliation:
Laboratory of Parasitology, Medical Faculty, University of Leiden, Wassenaarseweg 62, P.O. Box 9605, 2300 RC Leiden, The Netherlands
*
*Corresponding author. Tel: + 45 39 626168. Fax: + 45 39 626121.
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Summary

Circulating antigen levels and antibody responses in Schistosoma bovis-infected West African Dwarf goats were evaluated during infection and following treatment with praziquantel (60 mg/kg) 13 weeks post-infection. One day, 1 week and 4 weeks post-treatment, subgroups of goats were sacrificed and perfused for worm recovery. For comparison, parasite-free control animals were included. Blood and faecal samples were collected biweekly. Two gut-associated schistosome antigens, circulating cathodic and circulating anodic antigen (CCA and CAA) and 3 specific antibody responses (total Ig, IgG and IgM) were measured. For specific antibody detection, crude S. bovis adult worm and egg homogenates were used. The level of CCA in the infected groups was significantly elevated from the time of onset of egg excretion onwards. However, following treatment, the CCA litres dropped to control levels within 1 week post-treatment. Strong positive correlations were found between CCA levels and worm counts and faecal egg counts during peak egg excretion. The correlations of CAA and specific antibody litres to egg and worm counts were poor. The antibody responses were all significantly elevated in the infected goats during patency, but only marginally affected by the treatment. Hence, CCA proved to be superior by correlating strongly to the level of infection and by being a sensitive indicator of the effect of treatment.

Keywords

Schistosoma bovisgoalscirculatingantigensantibodyresponsespraziquanteltreatment
Type
Research Article
Information
Parasitology , Volume 113 , Issue 4 , October 1996 , pp. 367 - 375
Copyright
Copyright © Cambridge University Press 1996

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