Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-23T17:18:34.206Z Has data issue: false hasContentIssue false
  • English
  • Français

Evidence for the existence of ganglioside molecules on Pneumocystis carinii from human lungs

Published online by Cambridge University Press:  06 April 2009

M. Sorice ,
L. Lenti ,
R. Misasi ,
C. Contini ,
L. Cignarella ,
T. Griggi ,
V. Vullo  and
C. Masala
M. Sorice
Affiliation:
Clinics of Tropical and Infectious Disease, University of Rome, Rome, Italy
L. Lenti
Affiliation:
Department of Experimental Medicine, University of Rome, Rome, Italy
R. Misasi
Affiliation:
Department of Experimental Medicine, University of Rome, Rome, Italy
C. Contini
Affiliation:
Institute of Infectious Disease, University of Rome, Rome, Italy
L. Cignarella
Affiliation:
Institute of Infectious Disease, University of Rome, Rome, Italy
T. Griggi
Affiliation:
Clinics of Tropical and Infectious Disease, University of Rome, Rome, Italy
V. Vullo
Affiliation:
Institute of Infectious Disease, University of Rome, Rome, Italy
C. Masala
Affiliation:
Clinics of Tropical and Infectious Disease, University of Rome, Rome, Italy
Get access Rights & Permissions [Opens in a new window]

Extract

This study was undertaken to assess whether glycolipid antigens (particularly gangliosides) are associated with Pneumocystis carinii obtained from human lungs. Gangliosides were extracted, purified in high performance thin-layer chromatography and stained with resorcinol. Two resorcinol-positive bands, co-migrating with GM1 and GD1a were demonstrated, suggesting the existence of ganglioside molecules on P. carinii. No resorcinol-positive bands were revealed in the pulmonary control tissue. In addition, an antiserum obtained from rabbits immunized with P. carinii antigen reacted with gangliosides GM1, and GD1a, as revealed by a dot immunobinding assay. This reactivity was inhibited by first incubating the antiserum with ganglioside micelles. Furthermore, anti-glycosphingolipid antibodies (aGM1) reacted with the bands of 200 and 55 kDa of P. carinii antigen. These results suggest that ganglioside antigens expressed on P. carinii can trigger specific immune responses.

Keywords

gangliosidesPneumocystis cariniiglycolipids
Type
Research Article
Information
Parasitology , Volume 105 , Issue 1 , August 1992 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Colman, P. G., Nayack, R. C., Campbeli, I. L. & Eisenbarth, G. S. (1988). Binding of cytoplasmic islet cell antibodies is blocked by human pancreatic glycolipid. Diabetes 37, 645–52.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Stanley, G. H. S. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Formisano, S., Johnson, M. L., Lee, G., Aloj, S. M. & Edelhoc, H. (1979). Critical micelle concentrations of gangliosides. Biochemistry 18, 1119–24.Google Scholar
Gigliotti, F., Ballou, L. R., Hughes, W. T. & Mosley, B. D. (1988). Purification and initial characterization of a ferret Pneumocystis carinii surface antigen. Journal of Infectious Diseases 158, 848–54.CrossRefGoogle ScholarPubMed
Graves, D. C., McNabb, S. J. N., Ivey, M. H. & Worley, M. A. (1986). Development and characterization of monoclonal antibodies to Pneumocystis carinii. Infection and Immunity 51, 125–33.CrossRefGoogle ScholarPubMed
Kates, M. (1990). Glycolipids of high plants, algae, yeasts, and fungi. In Glycolipids, Phosphoglycolipids and Sulfoglycolipids, (ed. Kates, M.), pp. 235–308. New York and London: Plenum Press.CrossRefGoogle Scholar
Kiguchi, K., Henning-Chubb, B. & Huberman, E. (1990). Glycosphingolypid patterns of peripheral blood lymphocytes, monocytes, and granulocytes are cell specific. Journal of Biochemistry 107, 8–14.Google Scholar
Kovacs, J. A., Halpern, J. L., Swan, J. C., Moss, J., Parrillo, J. E. & Masur, H. (1988). Identification of antigens and antibodies specific for Pneumocystis carinii. Journal of Immunology 140, 2023–31.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Masala, C., Sorice, M., Di Prima, M. A., Lenti, L., Misasi, R., Mojon, M., Contini, C. & Vullo, V. (1989). Evidence for shared epitopes between cardiolipin and Pneumocystis carinii. Journal of Infectious Diseases 160, 736–7.CrossRefGoogle ScholarPubMed
Mojon, M. (1976). Serodiagnostic des affection à Pneumocystis carinii. Premiere resultats. Lyon, France: Universitè Claude Bernard.Google Scholar
Pesanti, E. L. (1987). Phospholipid profile of Pneumnocystis carinii and its interaction with alveolar type II epithelial cells. Infection and Immunity 55, 736–41.CrossRefGoogle Scholar
Pesanti, E. L. & Shanley, J. D. (1988). Glycoproteins of Pneumocystis carinii: characterization by electrophoresis and microscopy. Journal of Infectious Diseases 158, 1353–9.CrossRefGoogle ScholarPubMed
Sorice, M., Lenti, L., Misasi, R., Di Prima, M. A., Griggi, T., Cignarella, L., Vullo, V. & Masala, C. (1991). Antiglycosphingolipid antibodies in HIV infection. AIDS 5, 345–6.Google ScholarPubMed
Strayer, D. S., Hallman, M. & Merrit, T. A. (1991). Immunogenicity of surfactant. I. Human alveolar surfactant. Clinical Experimental Immunology 83, 35–40.CrossRefGoogle ScholarPubMed
Sut, A., Sirugue, S., Sixou, S., Lakhdar-Ghazai, F., Tocanne, J. F., Laneelle, G. (1990). Mycobacteria glycolipids as potential pathogenicity effectors: alteration of model and natural membranes. Biochemistry 29, 8498–502.CrossRefGoogle ScholarPubMed
Tanabe, K., Takasaki, S., Watanabe, J. I., Kobata, A., Egawa, K. & Nakamura, Y. (1989). Glycoproteins composed of major surface immunodeterminants of Pneumocystis carinii. Infection and Immunity 57, 1363–8.CrossRefGoogle ScholarPubMed
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.Google Scholar
Walzer, P. D., Rutledge, M. E., Yoneda, K., Stahr, B. J. (1979). Pneumocystis carinii: new separation method from lung tissue. Experimental Parasitology 47, 356–68.CrossRefGoogle ScholarPubMed
Williams, M. A. & McCluer, R. H. (1980). The use of Sep-pac C18 cartridges during the isolation of gangliosides. Journal of Neurochemistry 35, 266–9.CrossRefGoogle Scholar