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Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense

Published online by Cambridge University Press:  06 April 2009

P. A. O. Majiva ,
R. Hamers ,
N. Van Meirvenne  and
G. Matthyssens
P. A. O. Majiva
Affiliation:
International Laboratory for Research on Animal Diseases (ILARAD), P. O. Box 30709, Nairobi, Kenya Vrije Universiteit Brussel, Paardenstaat 65, 1640 Sint-Genesius-Rode, Belgium
R. Hamers
Affiliation:
Vrije Universiteit Brussel, Paardenstaat 65, 1640 Sint-Genesius-Rode, Belgium
N. Van Meirvenne
Affiliation:
Laboratorium voor Serology, Instituut voor tropische Geneeskunde, Nationalestraat 155, 2000 Antwerp, Belgium
G. Matthyssens
Affiliation:
Vrije Universiteit Brussel, Paardenstaat 65, 1640 Sint-Genesius-Rode, Belgium
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Summary

Genetic proximity between two karyotypic groups of Trypanosoma congolense was investigated using as hybridization probes: (i) total genomic DNA, (ii) a 35 nucleotide long synthetic oligonucleotide, and (iii) non-variant antigen type (non-VAT) specific complementary DNAs. The phylogenetic relationship between Trypanosoma brucei and T. evansi, both of which are accepted species in the subgenus Trypanozoon, was used as a reference to assess the phylogenetic proximity of the two groups of T. congolense. Results indicate that some morphologically indistinguishable T. congolense populations differ in a variety of molecular and genetic properties: molecular karyotypes, majority of the DNA sequences, and the restriction enzyme sites in the genomic environments of various conserved genes. The implications of these findings for trypanosome evolution and T. congolense epidemiology are discussed.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 291 - 304
Copyright
Copyright © Cambridge University Press 1986

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