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Evaluation of immunoglobulin G4 subclass antibody in a peptide-based enzyme-linked immunosorbent assay for the serodiagnosis of human fascioliasis

Published online by Cambridge University Press:  23 August 2007

C. TANTRAWATPAN ,
W. MALEEWONG ,
C. WONGKHAM ,
S. WONGKHAM ,
P. M. INTAPAN  and
K. NAKASHIMA
C. TANTRAWATPAN*
Affiliation:
Graduate Study Office, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, Thailand
W. MALEEWONG
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
C. WONGKHAM
Affiliation:
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
S. WONGKHAM
Affiliation:
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
P. M. INTAPAN
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
K. NAKASHIMA
Affiliation:
Faculty of Human Health, Tokai Gakuen University, Tenpaku-ku, Nagoya, Aichi, Japan
*
*Corresponding author: Graduate Study Office, Faculty of Medicine, Thammasat University, Rangsit Campus, Paholyothin Road, Klong-luang, Pathumthani 12121, Thailand. Tel: +66 29269767. Fax: +66 29269755. E-mail: [email protected]
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Summary

To improve the diagnosis of human fascioliasis caused by Fasciola gigantica, we developed a peptide-based enzyme-linked immunosorbent assay (peptide-based ELISA) based on the detection of specific IgG4 subclass antibody. Two identified B-cell epitopes of F. gigantica cathepsin L1 were synthesized as single synthetic peptides, acetyl-DKIDWRESGYVTELKDQGNC-carboxamide (peptide L) and acetyl-DKIDWRESGYVTEVKDQGNC-carboxamide (peptide V), and their diagnostic potential was evaluated. The sera of 25 patients infected with F. gigantica, 212 patients with other parasitic infections, 32 cholangiocarcinoma patients and 57 healthy controls were analysed. The sensitivity, specificity, accuracy, and positive and negative predictive values of this assay were the same with both peptides at 100%, 99·7%, 99·7%, 96·2% and 100%, respectively. These highly sensitive and specific peptide-based ELISAs for the detection of specific IgG4 antibody could be useful for laboratory diagnosis of human fascioliasis in future large-scale surveys throughout Southeast Asia where this disease is prevalent.

Keywords

fascioliasisFasciola giganticaIgG4peptide-based ELISAserodiagnosis
Type
Research Article
Information
Parasitology , Volume 134 , Issue 14 , December 2007 , pp. 2021 - 2026
Copyright
Copyright © Cambridge University Press 2007

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