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Estimation of relative fecundity in Eimeria tenella strains by a mixed infection method

Published online by Cambridge University Press:  06 April 2009

T. Nakamura ,
T. Konishi ,
H. Kawaguchi  and
J. Imose
T. Nakamura
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co. Ltd, Koka-cho, Shiga 520–34, Japan
T. Konishi
Affiliation:
Shionogi Research Laboratories, Shionogi and Co. Ltd, Fukushima-ku, Osaka 553, Japan
H. Kawaguchi
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co. Ltd, Koka-cho, Shiga 520–34, Japan
J. Imose
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co. Ltd, Koka-cho, Shiga 520–34, Japan
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Summary

The relative fecundity of populations of Eimeria tenella was estimated by means of mixed infection using electrophoretic variation of glucose-phosphate isomerases (GPIs) as a genetic marker. A decoquinate-resistant strain with GPI-9 isozyme (DR-NIAH), a decoquinate-sensitive one with GPI-1 (DS-Iwate), and three decoquinate-resistant lines (No. 3, 4, and 5) derived from cross-fertilization between DR-NIAH and DS-Iwate, were used. The GPI phenotypes of the No. 3 and No. 4 lines are GPI-9, and that of No. 5 is GPI-1. Mixed infection experiments were performed between DR-NIAH and DS-Iwate, No. 3 and No. 5, and No. 4 and No. 5. DR-NIAH was predominant over DS-Iwate in the mixed infection, whereas, in single infections, the total oocyst output of DR-NIAH was similar to or less than that of DS-Iwate. Among three lines, No. 4 was predominant over No. 5, and No. 5 was predominant over No. 3 in the mixed infection. Relative fecundity between No. 3 and No. 5 and their patterns of oocyst output in single infections were similar to those in the mixed infection. In contrast, No. 5 produced more oocysts than No. 4 in single infections, suggesting that the oocyst production in the mixed infection may be influenced by the mutual interference or competition between the populations of E. tenella in the chicken caeca.

Keywords

Eimeria tenellaglucose-phosphateisomerase isozymesmixed infection
Type
Research Article
Information
Parasitology , Volume 104 , Issue 1 , February 1992 , pp. 11 - 17
Copyright
Copyright © Cambridge University Press 1992

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