Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-23T14:24:17.990Z Has data issue: false hasContentIssue false
  • English
  • Français

Epitopes expressed on very low Mr Schistosoma mansoni adult tegumental antigens conform to a general pattern of life-cycle cross-reactivity

Published online by Cambridge University Press:  06 April 2009

A. J. G. Simpson ,
P. Hagan ,
F. Hackett ,
P. Omer Ali  and
S. R. Smithers
A. J. G. Simpson
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
P. Hagan
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
F. Hackett
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
P. Omer Ali
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
S. R. Smithers
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

The relationship between antigens associated with the surface of newly transformed schistosomula of Schistosoma mansoni and the tegumental surface membrane of adult S. mansoni worms has been further explored. Immunoprecipitation of detergent-solubilized 125I-tegumental surface membrane antigens of adult S. mansoni with antibodies from mice vaccinated with highly irradiated S. mansoni cercariae revealed major antigens of Mr 32, 20, 15 and 8K. The Mr 32 and 20K antigens have been previously demonstrated to be antigenically and electrophoretically identical to major antigens on the schistosomulum surface. The Mr 15 and 8K antigens, on the other hand, have not been identified by the immunoprecipitation of 125I-schistosomulum surface antigens, although a distinct schistosomulum surface antigen of Mr 15K is precipitated by antibodies from mice vaccinated with highly irradiated cercariae. Nevertheless, it was shown that antibodies to the Mr 15 and 8K antigens were specifically absorbed from vaccinated mouse serum by intact, live schistosomula, demonstrating that the Mr 15 and 8K antigens are exposed on or released from the schistosomulum surface. In contrast, absorption of the antiserum with eggs failed to remove antibody against any of the four tegumental membrane antigens examined. The Mr 15 and 8K antigens were shown to be recognized via polypeptide epitopes and not periodate-sensitive carbohydrate epitopes, further emphasizing the similarity of these to the well-characterized Mr 32 and 20K tegumental surface membrane antigens. A general relationship between schistosomulum surface, adult tegumental membrane and egg antigens was demonstrated by ELISA, using antibodies raised against the three antigenic fractions. It was shown that both the egg and adult tegumental membrane antigens cross-react with the schistosomulum surface, but that the egg and adult membrane antigens exhibit very little, if any, mutual cross-reactivity. This antigen divergence possibly enables the host to dissociate pathological, anti-egg responses from potentially protective anti-membrane responses during the course of natural infection. It also suggests that adult membrane antigens could be used in an anti-schistosome vaccine without the possible complication of inducing pathological responses.

Keywords

Schistosoma mansonitegumental antigensepitopesschistosomulum surface antigensegg antigens
Type
Research Article
Information
Parasitology , Volume 100 , Issue 1 , February 1990 , pp. 73 - 81
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Fraker, P. J. & Speck, J. C. Jr. (1987). Protein and cell membrane iodination with a sparingly soluble chloramide, l,3,4,6-tetrachloro-3a,6a-diphenyl-glycoluril. Biochemical and Biophysical Research Communications 80, 849–57.CrossRefGoogle Scholar
Kelly, C. (1987). Molecular studies of schistosome immunity. In The Biology of Schistosomes (ed. Rollinson, D. & Simpson, A. J. G.), pp. 265–293. London and New York: Academic Press.Google Scholar
Knight, M., Simpson, A. J. G., Payares, G., Chaudhri, M. & Smithers, S. R. (1984). Cell-free synthesis of Schistosoma mansoni surface antigens: stage specificity of their expression. EMBO Journal 3, 213–19.CrossRefGoogle ScholarPubMed
Knight, M., Kelly, C., Rodrigues, V., Yi, X., Wamachi, A., Smithers, S. R. & Simpson, A. J. G. (1989). A cDNA clone encoding part of the major Mr 25000 surface membrane antigen of adult Schistosoma mansoni. Parasitology Research 75, 281–6.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.Google Scholar
Omer Ali, P., Magee, A. I., Kelly, C. & Simpson, A. J. G. (1986). A major role for carbohydrates preferentially recognised by chronically infected mice in the determination of Schistosoma mansoni schistosomulum surface antigenicity. Journal of Immunology 137, 3601–7.CrossRefGoogle Scholar
Omer Ali, P., Smithers, S. R., Bickle, Q., Phillips, S. M., Harn, D. & Simpson, A. J. G. (1988). Analysis of the anti-Schistosoma mansoni surface antibody response during murine infection and its potential contribution to protective immunity. Journal of Immunology 140, 3273–9.Google Scholar
Omer Ali, P., Hagan, P., Hackett, F., Smithers, S. R. & Simpson, A. J. G. (1989 a). Variable species and stage specificity of schistosomulum surface epitopes recognized by mice vaccinated with highly irradiated cercariae. Parasite Immunology 11, 301–18.Google ScholarPubMed
Omer Ali, P., Mansour, M., Woody, J. N., Smithers, S. R. & Simpson, A. J. G. (1989 b). Antibody to carbohydrate and polypeptide epitopes on the surface of schistosomula of Schistosoma mansoni in Egyptian patients with acute and chronic schistosomiasis. Parasitology 98, 417–24.CrossRefGoogle ScholarPubMed
Payares, G., McLaren, D. J., Evans, W. H. & Smithers, S. R. (1985 a). Antigenicity and immunogenicity of the tegumental outer membrane of adult Schistosoma mansoni. Parasite Immunology 7, 45–61.CrossRefGoogle ScholarPubMed
Payares, G., Kelly, C., Smithers, S. R. & Evans, W. H. (1985 b). Evidence that the 32, 38 and 20 kilodalton surface antigens of schistosomula and schistosomes are a family of conserved proteins. Molecular and Biochemical Parasitology 17, 115–30.CrossRefGoogle Scholar
Payares, G., McLaren, D. J., Evans, W. H. & Smithers, S. R. (1985 c). Changes in the surface antigen profile of Schistosoma mansoni during maturation from cercaria to adult worm. Parasitology 91, 83–99.CrossRefGoogle ScholarPubMed
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Fiqueredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: defined system for stepwise transformation of cercariae to schistosomula in vitro. Experimental Parasitology 36, 360–72.CrossRefGoogle Scholar
Simpson, A. J. G., Hackett, F., Walker, T., Derossi, R. & Smithers, S. R. (1985). Antibody response against schistosomulum surface antigens and protective immunity following immunization with highly irradiated cercariae. Parasite Immunology 7, 133–52.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.Google Scholar
Smithers, S. R., Hackett, F., Omer Ali, P. & Simpson, A. J. G. (1989). Protective immunization of mice against Schistosoma mansoni with purified adult worm surface membranes. Parasite Immunology 11, 257–67.CrossRefGoogle ScholarPubMed
Von Lichtenberg, F. (1987). Consequences of infection with schistosomes. In The Biology of Schistosomes (ed. Rollinson, D. & Simpson, A. J. G.), pp. 185–232. London and New York: Academic Press.Google Scholar