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Enzyme polymorphism of freshwater fish trypanosomes and its use for strain identification

Published online by Cambridge University Press:  06 April 2009

P. Zajíček
P. Zajíček
Affiliation:
Institute of Parasitology, Czechoslovak Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czechoslovakia
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Summary

Sixteen strains of trypanosomes isolated from the blood of 10 freshwater fish species (all but one from South Bohemia) were characterized by enzyme analysis using dise electrophoresis in 5% polyacrylamide gel. Six enzymes were selected: ADH, LDH, MDH, ME, G6PDH and GPI. The most variable were ADH and LDH, whereas MDH, G6PDH and GPI showed the same pattern in all strains analysed. Cluster analysis indicated a very high degree of similarity among strains, even those isolated from phylogenetically distant hosts. Moreover, no correlation between enzyme pattern and host species was found.

Keywords

fish trypanosomesenzyme polymorphismstrain identification
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 221 - 224
Copyright
Copyright © Cambridge University Press 1991

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