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Endotoxins in the blood and cerebrospinal fluid of patients with African sleeping sickness

Published online by Cambridge University Press:  06 April 2009

V. W. Pentreath ,
R. A. Alafiatayo ,
B. Crawley ,
F. Doua  and
E. A. Oppenheim
V. W. Pentreath
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
R. A. Alafiatayo
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
B. Crawley
Affiliation:
Microbiology Department, Public Health Laboratory, West Didsbury, Manchester M20 2LR
F. Doua
Affiliation:
Projet de Recherches Cliniques sur la Trypanosomiase, BP 1425 Daloa, République de Côte d'lvoire
E. A. Oppenheim
Affiliation:
Microbiology Department, Public Health Laboratory, West Didsbury, Manchester M20 2LR
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Summary

Endotoxin levels were measured in the blood and cerebrospinal fluid (CSF) of control individuals and 2 groups of patients with African sleeping sickness. Endotoxin levels were markedly elevated in the blood (infected groups mean endotoxin values 40·2 pg/ml and 53·8 pg/ml, compared to control 11·6 pg/ml, P < 0·0001 for both increases) and CSF (infected groups mean endotoxin values 45·8 pg/ml and 50·1 pg/ml compared to control 6·3 pg/ml, P < 0·0001 for both increases) of the patients. The levels were reduced 6 weeks following different drug treatments in the 2 groups (blood levels to mean 33·8·pg/ml and 28·5 pg/ml; CSF levels to 37·4 pg/ml and 27·0 pg/ml). The blood endotoxin values correlated with the CSF values before treatment (r = 0·74 and 0·57 for the 2 groups; P < 0·0001 for both) and after treatment (r = 0·57 and 0·56 for the 2 groups; P < 0·0001 for both). It is concluded that raised endotoxin equilibrates in the blood and CSF compartments, and may contribute significantly to the pathology of sleeping sickness.

Keywords

African sleeping sicknessendotoxinpathogenesiscerebrospinal fluid.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 1 , January 1996 , pp. 67 - 73
Copyright
Copyright © Cambridge University Press 1996

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References

REFERENCES

Alafiatayo, R. A., Crawley, B., Oppenheim, B. A. & Pentreath, V. W. (1993). Endotoxins and the pathogenesis of Trypanosoma brucei brucei infections in mice. Parasitology 107, 49–53.CrossRefGoogle ScholarPubMed
Bakhiet, A.M. O. (1993). Immunopathogenesis of Experimental African Trypanosomias. Interactions between Trypanosoma brucei brucei, CD8 +T Cells and Interferon Gamma. Stockholm: Karolinska Institute.Google Scholar
Bate, C. A. W., Taverne, J., Roman, E., Moreno, C. & Playfair, J. H. L. (1992). Tumour necrosis factor induction by malaria exoantigens depends upon phospholipid. Immunology 75, 129–35.Google Scholar
Burroughs, M., Cabellos, C., Prasad, S. & Tuomanen, E. (1992). Bacterial components and the pathophysiology of injury to the blood–brain barrier: does cell wall add to the effects of endotoxin in gram-negative meningitis ? Journal of Infectious Diseases 165 (Suppl. 1), 582–5.CrossRefGoogle Scholar
Corriveau, C. C. & Danner, R. L. (1993). Endotoxin as a therapeutic target in septic shock. Infectious Agents and Disease 2, 35–43.Google ScholarPubMed
Greenwood, B. M. (1974). Possible role of a β-cell mitogen in hyper-gammaglobulinaemia in malaria and trypanosomiasis. Lancet 1, 435–6.CrossRefGoogle Scholar
Jakobsen, P. H., Baek, L. & Jepsen, S. (1988). Demonstration of soluble Plasmodium falciparum antigens reactive with Limulus amoebocyte lysate and polymyxin B. Parasite Immunology 10, 593–606.Google Scholar
Ketteridge, D. S. (1978). Lipopolysaccharide from Trypanosoma cruzi. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 101–2.CrossRefGoogle ScholarPubMed
Lambert, P. H., Berney, M. & Kazyumba, G. (1981). Immune complexes in serum and cerebrospinal fluid in African trypanosomiasis. Correlation with polyclonal β cell activation and with intracerebral immunoglobulin synthesis. Journal of Clinical Investigation 67, 77–85.Google Scholar
Lucas, R., Magez, S., Songa, B., Darji, A., Hamers, R. & De Baetselier, P. (1993). A role for TNF during African trypanosomiasis: involvement in parasite control, immunosuppression and pathology. Research in Immunology 144, 370–6.Google Scholar
Martich, G. D., Boujoukos, A. J. & Suffredini, A. F. (1993). Responses of man to endotoxin. Immunobiology 187, 403–16.CrossRefGoogle Scholar
McCartney, A. C., Robertson, M. R. I., Piotrowicz, B. I. & Lucie, N. P. (1987). Endotoxaemia, fever and clinical states in immunosuppressed patients: a preliminary report. Journal of Infection 15, 201–6.Google Scholar
Morita, T., Tanaka, S., Nakamura, T. & Iwanaga, S. (1981). A new (l-3)-beta-D-glucan mediated coagulation pathway found in Limulus amoebocytes. FEBS Letters 129, 318–21.Google Scholar
Ndung'u, J. M., Ngure, R. M., Ngotho, J. M., Sayet, P. D. & Omuse, J. K. (1995). Total protein and white cell changes in the cerebrospinal fluid of vervet monkey infected with Trypanosoma rhodesiense and the post-treatment reaction. Japanese Journal of Protozoology Research (In Press.)Google Scholar
Pentreath, V. W. (1994). Endotoxins and their significance for murine trypanosomiasis. Parasitology Today 10, 226–9.Google Scholar
Pentreath, V. W., Baugh, P. J. & Lavin, D. R. (1994). Sleeping sickness and the central nervous system. Onderstepoort Journal of Veterinary Research 61, 369–77.Google ScholarPubMed
Philip, K. A., Dascombe, M. J., Fraser, P. A. & Pentreath, V. W. (1994). Blood-brain barrier damage in experimental trypanosomiasis. Annals of Tropical Medicine and Parasitology 88, 607–16.CrossRefGoogle Scholar
Rietschel, E. T. & Brade, H. (1992). Bacterial endotoxins. Scientific American 267, 26–33.Google Scholar
Singer, I., Kimble, E. T. & Ritts, R. E. (1964). Alterations in the host-parasite relationship by administration of endotoxin to mice with infections of trypanosomes. Journal of Infectious Diseases 14, 243–8.CrossRefGoogle Scholar
Styles, T. J. (1965). Effect of bacterial endotoxin on Trypanosoma lewisi infections in rats. Journal of Parasitology 51, 650–3.Google Scholar
Van Deventer, S. J. H., Pauw, W., Ten Cate, J. W., Janssen, M. E., Büller, H. R. & Sturk, A. (1987). Clinical evaluation in febrile patients of an optimized endotoxin assay in blood. Progress in Clinical and Biological Research 231, 489–98.Google Scholar