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Endopeptidases from Plasmodium knowlesi

Published online by Cambridge University Press:  06 April 2009

E. Hempelmann  and
R. J. M. Wilson
E. Hempelmann
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
R. J. M. Wilson
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Extracts of rhesus monkey erythrocytes infected with Plasmodium knowlesi were fractionated by polyacrylamide gel electrophoresis (PAGE) and several zones of endopeptidase activity were demonstrated by an imprint-digest method. The enzymes were active only under acid conditions; activity was detected at pH 3·2 but not between pH 6·4 and 8·9 using haemoglobin, albumin or erythrocyte lysate as the substrate. Optimized PAGE conditions separated highly active parasite enzymes with Rf values of 73, 63 and 53 (± 7%), as well as a red cell endopeptidase, Rf44. Of two other minor bands of activity, one was associated with platelets.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 2 , April 1980 , pp. 323 - 330
Copyright
Copyright © Cambridge University Press 1980

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