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Eimeria species: studies using rRNA and rDNA probes

Published online by Cambridge University Press:  06 April 2009

J. Ellis  and
J. Bumstead
J. Ellis
Affiliation:
Department of Parasitology, AFRC Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambs PE17 2DA
J. Bumstead
Affiliation:
Department of Parasitology, AFRC Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambs PE17 2DA
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Summary

rRNA and a heterologous cloned rDNA probe have been used to detect the rRNA genes of Eimeria species which infe the chicken, and has allowed the isolation and preliminary characterization of cloned rDNA sequences from a genomic DNA library of Eimeria tenella. It is demonstrated that rRNA and rDNA probes can be used to identify individual Eimeria species by the restriction fragment patterns detected after Southern hybridization. In addition, studies have shown that the large and small subunit rRNAs are expressed throughout sporulation.

Keywords

Eimeria spp.rRNArDNADNA probes
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1990

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