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Effects of multiple stressors on northern leopard frogs in agricultural wetlands

Published online by Cambridge University Press:  09 March 2021

David J. Marcogliese Open the ORCID record for David J. Marcogliese [Opens in a new window] ,
Kayla C. King  and
Kieran A. Bates
David J. Marcogliese*
Affiliation:
Aquatic Contaminants Research Division, Water Science and Technology Directorate, Science and Technology Branch, Environment and Climate Change Canada, St. Lawrence Centre, 105 McGill Street, 7th floor, Montreal, Quebec H2Y 2E7, Canada St. Andrews Biological Station, Fisheries and Oceans Canada, 125 Marine Science Drive, St. Andrews, New Brunswick E5B 0E4, Canada
Kayla C. King
Affiliation:
Department of Biology, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, Quebec H3G 1M8, Canada Department of Zoology, University of Oxford, 11a Mansfield Road, Oxford OX1 3SZ, UK
Kieran A. Bates
Affiliation:
Department of Zoology, University of Oxford, 11a Mansfield Road, Oxford OX1 3SZ, UK
*
Author for correspondence: David J. Marcogliese, E-mail: [email protected]
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Abstract

Natural and anthropogenic stressors, including parasites and pesticides, may induce oxidative stress in animals. Measuring oxidative stress responses in sentinel species that are particularly responsive to environmental perturbations not only provides insight into host physiology but is also a useful readout of ecosystem health. Newly metamorphosed northern leopard frogs (Lithobates pipiens), a sentinel species, were collected from agricultural and non-agricultural wetlands exposed to varying concentrations of the herbicide atrazine. Significant effects of certain parasites' abundance and their interaction with atrazine exposure on frog oxidative stress were identified. Specifically, increased protein levels were detected in frogs infected with echinostome metacercariae. In addition, the nematode Oswaldocruzia sp. was significantly associated with increased thiol concentration and catalase activity. Significant parasite × atrazine interactions were observed for atrazine exposure and the abundance of Oswaldocruzia sp. on thiol, as thiol concentrations increased with parasite abundance at low atrazine localities and decreased in high atrazine wetlands. In addition, a significant interaction between the abundances of Oswaldocruzia sp. and gorgoderid trematodes on thiol concentrations was observed. These findings demonstrate that studies of oxidative stress on animals in natural ecosystems should account for the confounding effects of parasitism, particularly for amphibians in agricultural landscapes.

Keywords

AmphibiansatrazineLithobates pipienslysozymenorthern leopard frogsoxidative stressparasitism
Type
Research Article
Information
Parasitology , Volume 148 , Issue 7 , June 2021 , pp. 827 - 834
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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