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Effects of irradiation on surface carbohydrates of larvae of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

A. Wales ,
S.-I. Fukumoto ,
M. F. Otieno  and
J. R. Kusel
A. Wales
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland
S.-I. Fukumoto
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland
M. F. Otieno
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland
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Summary

Ultra-violet irradiated larvae of Schistosoma mansoni stimulate high levels of resistance to challenge infection in experimental animals. In the experiments presented here, the binding patterns of antisera specific for the cercarial glycocalyx, and of various lectins, demonstrate that u.v. irradiation causes a pronounced modification of the carbohydrate antigens expressed at the surface of cercariae and newly transformed schistosomula.These alterations were dependent on the irradiation dose, and on the batch of cercariae used in each experiment. Our results strongly suggest that the changes in carbohydrate antigens consequent upon u.v. irradiation may be important in generating the enhanced immunogenicity of irradiated cercariae.

Keywords

Schistosoma mansoniu.v. irradiationirradiated vaccineglycocalyxcarbohydrate antigensimmunity.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 2 , February 1993 , pp. 117 - 125
Copyright
Copyright © Cambridge University Press 1993

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