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Effect of temperature on Wolbachia density and impact on cytoplasmic incompatibility

Published online by Cambridge University Press:  13 September 2005

L. MOUTON ,
H. HENRI ,
M. BOULETREAU  and
F. VAVRE
L. MOUTON
Affiliation:
Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
H. HENRI
Affiliation:
Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
M. BOULETREAU
Affiliation:
Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
F. VAVRE
Affiliation:
Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Lyon 1, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
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Abstract

The outcome and the evolution of host-symbiont associations depend on environmental constraints, but responses are difficult to predict since they arise from a complex interaction between the host, the parasite and the environment. The situation can be even more complex when multiple parasite genotypes, with potentially different responses to environmental changes, coexist within a single host. In this paper, we investigated the effect of the temperature (from 14 to 26 °C) during the host development on the density of 3 strains of the intracellular bacterium Wolbachia that coexist within the wasp Leptopilina heterotoma. In this species, Wolbachia induces cytoplasmic incompatibility, a sperm-egg incompatibility that allows it to spread and persist in host populations. Using real-time quantitative PCR we found that (i) Wolbachia density is temperature-specific and highest at 26 °C; (ii) the order of the abundance of the 3 Wolbachia strains does not vary with temperature changes; (iii) the response of bacterial density to temperature occurs within a single insect generation, during the egg-to-adult developmental period; (iv) in this species, temperature-related changes in Wolbachia density do not influence cytoplasmic incompatibility.

Keywords

temperatureWolbachiadensityLeptopilina heterotomacytoplasmic incompatibilitysymbiosis
Type
Research Article
Information
Parasitology , Volume 132 , Issue 1 , January 2006 , pp. 49 - 56
Copyright
© 2005 Cambridge University Press

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