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Effect of metacestodes of Hymenolepis diminuta on storage and circulating carbohydrates in the intermediate host, Tenebrio molitor

Published online by Cambridge University Press:  06 April 2009

J. Y. Kearns ,
H. Hurd  and
A. S. Pullin
J. Y. Kearns
Affiliation:
Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
H. Hurd
Affiliation:
Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
A. S. Pullin
Affiliation:
Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
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Summary

The metamorphosis of oncospheres of the rat tapeworm, Hymenolepis diminuta, to mature metacestodes induces several pathophysiological effects in the intermediate host, Tenebrio molitor (Coleoptera). Previous investigations have failed to elucidate the mechanism responsible for changes in the host reproductive physiology and behaviour. This work forms part of an assessment of the degree to which nutrient resource management may be involved in these interactions. We report that developing metacestodes exert a pronounced effect upon host carbohydrate reserves and also alter circulating carbohydrate titres at some stages post-infection. Biochemical studies of fat body glycogen demonstrated a significant depletion of reserves as early as 3 days post-infection in males and 5 days post-infection in females. Gas chromatography revealed trehalose to be the predominant haemolymph carbohydrate, titres being significantly elevated in male beetles 5 and 9 days post-infection and in females 12 days post-infection. Metacestodes had no effect on glucose concentrations in male or female beetles.

Keywords

Hymenolepis diminutaTenebrio molitormetacestodeshost carbohydratesfat body
Type
Research Article
Information
Parasitology , Volume 108 , Issue 4 , May 1994 , pp. 473 - 478
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Arme, C. (1988). Ontogenetic changes in helminth membrane function. Parasitology 96, S83–S104.CrossRefGoogle ScholarPubMed
Bahjou, A., Gourdoux, L., Moreau, R. & Dutrieu, J. (1988). In vitro regulation of glycogen phosphorylase of the larval fat body of Tenebrio molitor. Comparative Biochemistry and Physiology 89B, 233–7.Google Scholar
Bedford, J. J. (1977). The carbohydrate levels of insect haemolymph. Comparative Biochemistry and Physiology 57A, 83–6.CrossRefGoogle Scholar
Fell, R. D. (1990). The qualitative and quantitative analysis of insect haemolymph sugars by high performance thin layer chromatography. Comparative Biochemistry and Physiology 95A, 539–44.CrossRefGoogle Scholar
Gäde, G. (1988). Studies on the hypertrehalosaemic factor from the corpus cardiacum/corpus allatum complex of the beetle, Tenebrio molitor. Comparative Biochemistry and Physiology 91A, 333–8.CrossRefGoogle Scholar
Gourdoux, L., Lequellec, Y., Moreau, R. & Dutrieu, J. (1983). Glucogenesis from some amino acids and its endocrine modification in Tenebrio molitor L. (Coleoptera). Comparative Biochemistry and Physiology 74B, 273–6.Google Scholar
Gourdoux, L., Moreau, R., Lequellec, Y. & Dutrieu, J. (1984). Glucogenesis from alanine during starvation of adult Tenebrio molitor L. (Coleoptera). Molecular Physiology 5, 35–40.Google Scholar
Hartfield, P. J., Williams, K. E., Geddes, R. & Lloyd, J. B. (1989). Glycogen metabolism in the rat visceral yolk sac. 1. Glycogen content and gestational age. Placenta 10, 45–54.Google Scholar
Heyneman, D. & Voge, M. (1957). Glycogen distribution in cysticercoids of three Hymenolepid cestodes. Journal of Parasitology 43, 527–30.CrossRefGoogle Scholar
Hurd, H. (1990). Physiological and behavioural interactions between parasites and invertebrate hosts. In Advances in Parasitology, Vol. 29 (ed. Baker, J. R. & Muller, R.), pp. 271–318. London: Academic Press.Google Scholar
Hurd, H. (1993). Reproductive disturbances induced by parasites and pathogens of insects. In Parasites and Pathogens of Insects, Vol. 1 (ed. Beckage, N. E., Thompson, S. N. & Federici, B. A.), pp. 87–105. San Diego: Academic Press Inc.CrossRefGoogle Scholar
Hurd, H. & Arme, C. (1984). Tenebrio molitor (Coleoptera): effect of metacestodes of Hymenolepis diminuta (Cestoda) on haemolymph amino acids. Parasitology 89, 245–51.CrossRefGoogle Scholar
Hurd, H. & Arme, C. (1986). Hymenolepis diminuta: influence of metacestodes on synthesis and secretion of fat body protein and its ovarian sequestration in the intermediate host, Tenebrio molitor. Parasitology 93, 111–20.Google Scholar
Hurd, H. & Arme, C. (1987). Hymenolepis diminuta (Cestoda): the role of intermediate host sex in the establishment, growth and development of metacestodes in Tenebrio molitor (Coleoptera). Helminthologia 24, 23–31.Google Scholar
Hurd, H. & Fogo, S. (1991). Hymenolepis diminuta induced changes in the behaviour of the intermediate host Tenebrio molitor. Canadian Journal of Zoology 69, 2291–4.Google Scholar
Kilby, B. A. (1963). The biochemistry of insect fat body. In Advances in Insect Physiology (ed. Beament, J. W. L., Treherne, J. E. & Wigglesworth, V. B.), pp. 111–174. New York and London: Academic Press.Google Scholar
Lethbridge, R. C. (1971). The hatching of Hymenolepis diminuta eggs and penetration of the hexacanths in Tenebrio molitor. Parasitology 62, 445–56.CrossRefGoogle ScholarPubMed
Matthews, J. R. & Downer, R. G. H. (1973). Hyperglycaemia induced by anaesthesia in the American cockroach, Periplaneta americana L. Canadian Journal of Zoology 51, 395–7.CrossRefGoogle Scholar
Pappas, P. W. & Durka, G.M. (1993 a). A study of carbohydrate metabolism in the eggs (oncospheres) of the tapeworm, Hymenolepis diminuta, with special reference to glucose. Parasitology 106, 201–9.CrossRefGoogle ScholarPubMed
Pappas, P. W. & Durka, G. M. (1993 b). Temporal changes in glucose and carbohydrate metabolism in the oncospheres of the cyclophyllidean tapeworm Hymenolepis diminuta. Parasitology 106, 317–27.Google Scholar
Phillip, A. A. (1985). Hymenolepis diminuta: monosaccharide absorption by the cysticercoid. Ph.D. thesis, University of Keele.Google Scholar
Pullin, A. S. (1992). Diapause metabolism and changes in carbohydrates related to cryoprotection in Pieris brassicae. Journal of Insect Physiology 38, 319–27.CrossRefGoogle Scholar
Pullin, A. S. & Bale, J. S. (1989). The effects of low temperature on diapausing Aglais urticae and Inachis io (Lepidoptera: Nymphalidae): overwintering physiology. Journal of Insect Physiology 35, 283–90.CrossRefGoogle Scholar
Thompson, S. N. (1985). Metabolic integration during host associations of multicellular animal endoparasites. Comparative Biochemistry and Physiology 81B, 21–42.Google Scholar
Voge, M. & Heyneman, D. (1957). Development of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Hymenolepidae) in the intermediate host Tribolium confusum. University of California Publications in Zoology 59, 549–80.Google Scholar
Wyatt, G. R. (1967). The biochemistry of sugars and polysaccharides in insects. Advances in Insect Biochemistry 4, 287–360.Google Scholar