Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-20T03:32:17.618Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of inulin on new and on patent infections of Trichuris suis in growing pigs

Published online by Cambridge University Press:  11 October 2006

S. PETKEVIČIUS ,
L. E. THOMSEN ,
K. E. BACH KNUDSEN ,
K. D. MURRELL ,
A. ROEPSTORFF  and
J. BOES
S. PETKEVIČIUS
Affiliation:
Department of Infectious Diseases, Lithuanian Veterinary Academy, Tilžės 18, LT-47181 Kaunas, Lithuania
L. E. THOMSEN
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark
K. E. BACH KNUDSEN
Affiliation:
Department of Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
K. D. MURRELL
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark
A. ROEPSTORFF
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Pathobiology, Royal Veterinary and Agricultural University, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark
J. BOES
Affiliation:
The National Committee for Pig Production, Danish Bacon & Meat Council, DK-8620 Kjellerup, Denmark
Get access Rights & Permissions [Opens in a new window]

Abstract

The objective of this experiment was to investigate the potential influence of inulin on the establishment of new and patent infections of Trichuris suis in growing pigs. Two experimental diets were formulated based on barley flour with either added insoluble fibre from oat husk (Diet 1) or a pure inulin (16%) supplementation (Diet 2). Twenty-eight 10-week-old pigs were divided randomly into 4 groups (Groups 1–4) each of 7 pigs. After 3 weeks adaptation to the experimental diets all pigs were infected with a single dose of 2000 infective T. suis eggs. Group 1 was fed Diet 1 until 7 weeks post-infection (p.i.) and Group 3 until 9 weeks p.i., Group 2 was fed Diet 2 until 7 weeks p.i., Group 4 was fed Diet 1 until week 7 p.i. and was switched-over from Diet 1 to Diet 2 until week 9 p.i. Seven weeks p.i. pigs in Groups 1 and 2 were slaughtered, and pigs in Groups 3 and 4 were slaughtered at 9 weeks p.i. Trichuris suis worm burdens were determined for all pigs. Inulin-fed pigs (Group 2) exhibited an 87% reduction in EPG, compared to the pigs on standard diet (Group 1) (P<0·0001). The number of worms recovered at week 7 p.i. from pigs on the inulin diet (Group 2) was significantly reduced by 71%, compared to the pigs on standard diet (Group 1) (P<0·01). At week 9, worm recovery in pigs on the inulin diet switch protocol (Group 4) was reduced by 47% compared to the control pigs in Group 3 (P<0·01). Further, the inulin-fed pigs exhibited a significant reduction in female worm fecundity and worm large intestine location was more distal compared to those from pigs on standard diet. These results demonstrate that inclusion of the highly degradable fructose polymer inulin in the diet leads to significant reductions in T. suis establishment, egg excretion, and female worm fecundity and can be used as a treatment for patent infections.

Keywords

Trichuris suisinulinnutritionpigs
Type
Research Article
Information
Parasitology , Volume 134 , Issue 1 , January 2007 , pp. 121 - 127
Copyright
© 2006 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bach Knudsen, K. E. ( 2001). Development of antibiotic resistance and options to replace antimicrobials in animal diets. Proceedings of the Nutrition Society 60, 291–299.Google Scholar
Bach Knudsen, K. E. and Hessov, I. ( 1995). Recovery of inulin from Jerusalem artichoke (Helianthus tuberosus L.) in the small intestine of man. British Journal of Nutrition 74, 101–113.Google Scholar
Bach Knudsen, K. E., Petkevičius, S., Jørgensen, H. and Murrell, K. D. ( 2003). A high load of rapidly fermentable carbohydrates reduces worm burden in infected pigs. In Manipulating Pig Production IX ( ed. Paterson, J. E.), pp. 169–170. Australasian Pig Science Association, Werribee, Australia.
Beer, R. J., Lean, I. J., Jacobs, D. E. and Curran, M. K. ( 1973). Clinical trichuriasis produced experimentally in growing pigs. Part II: Anthelmintic efficacy of dichlorvos. Veterinary Record 93, 195–197.Google Scholar
Bornet, F. R. J., Brouns, F., Tashiro, Y. and Duliver, V. ( 2002). Nutritional aspects of short-chain oligosaccharides: natural occurrence, chemistry, physiology and health implications. Digestive and Liver Disease 34, S111–S120.CrossRefGoogle Scholar
Crompton, D. W. T. ( 1999). How much human helminthiasis is there in the world? Journal of Parasitology 85, 397–403.Google Scholar
Cummings, J. H., Robertfroid, M. B., Anderson, H., Barth, C. A., Ferro-Luzzi, A., Ghoos, Y., Gibney, M., Hermansen, K., James, W. P. T., Korver, O., Lairon, D., Pascal, G. and Voragen, A. G. S. ( 1997). A new look at dietary carbohydrate: chemistry, physiology and health. European Journal of Clinical Nutrition 51, 417–423.CrossRefGoogle Scholar
Holland, C. V. ( 1987). Neglected infections – trichuriasis and strongyloidiasis. In Impact of Helminth Infections on Human Nutrition ( ed. Stephenson, L. S. and Holland, C. V.), pp. 161–201. Taylor and Francis, London, New York and Philadelphia.
Kelly-Quagliana, K. A., Nelson, P. D. and Buddington, R. K. ( 2003). Dietary oligofructose and inulin modulate immune functions in mice. Nutrition Research 23, 257–267.CrossRefGoogle Scholar
Meyer, D. P., Tungland, B. C., Causey, J. L. and Slavin, J. L. ( 2001). In vitro- und in vivo- Effekte von Inulin auf das Immunsystem. Ernährungs-Umschau 48, 13–16.Google Scholar
Morris, R. G., Jordan, H. E., Luce, W. G., Coburn, T. C. and Maxwell, C. V. ( 1984). Prevalence of gastrointestinal parasitism in Oklahoma Swine. American Journal of Veterinary Research 45, 2421–2423.Google Scholar
Pattison, H. D., Thomas, R. J. and Smith, W. C. ( 1980). A survey of gastrointestinal parasitism in pigs. Veterinary Record 107, 415–418.CrossRefGoogle Scholar
Pearce, G. P. ( 1999). Interactions between dietary fibre, endo-parasites and Lawsonia intracellularis bacteria in grower-finisher pigs. Veterinary Parasitology 87, 51–61.CrossRefGoogle Scholar
Pedersen, S. ( 2002). Population biology of Trichuris suis in pigs in relation to host nutritional status and its relevance to human T. trichuria infections. Ph.D. thesis, University of Copenhagen, Denmark.
Pedersen, S. and Saeed, I. ( 2001). Acquired immunity to Trichuris suis infection in pigs. Parasitology 123, 95–101.CrossRefGoogle Scholar
Pedersen, S. and Saeed, I. ( 2002). Host age influence on the intensity of experimental Trichuris suis in pigs. Parasite 9, 75–79.CrossRefGoogle Scholar
Petkevičius, S., Bach Knudsen, K. E., Nansen, P. and Murrell, K. D. ( 2001). The effect of dietary carbohydrates with different digestibility on the populations of Oesophagostomum dentatum in the intestinal tract of pigs. Parasitology 123, 315–324.CrossRefGoogle Scholar
Petkevičius, S., Bach Knudsen, K. E., Nansen, P., Roepstorff, A., Skjøth, F. and Jensen, K. ( 1997). The impact of diets varying in carbohydrates resistant to endogenous enzymes and lignin on populations of Ascaris suum and Oesophagostomum dentatum in pigs. Parasitology 114, 555–568.Google Scholar
Petkevičius, S., Bach Knudsen, K. E. and Murrell, K. D. ( 2003 b). Effects of Oesophagostomum dentatum and dietary carbohydrates on morphology of the large intestine of pigs. Veterinary Parasitology 116, 125–138.Google Scholar
Petkevičius, S., Bach Knudsen, K. E., Murrell, K. D. and Wachmann, H. ( 2003 a). The effect of inulin and sugar beet fibre on Oesophagostomum dentatum infection in pigs. Parasitology 127, 61–68.Google Scholar
Petkevičius, S., Bjørn, H., Roepstorff, A., Nansen, P., Bach Knudsen, K. E., Barnes, E. H. and Jensen, K. ( 1995). The effect of two types of diet on populations of Ascaris suum and Oesophagostomum dentatum in experimentally infected pigs. Parasitology 111, 395–402.CrossRefGoogle Scholar
Petkevičius, S., Murrell, K. D., Bach Knudsen, K. E., Jørgensen, H., Roepstorff, A., Laue, A. and Wachmann, H. ( 2004). Effects of short-chain fatty acids and lactic acids on survival of Oesophagostomum dentatum in pigs. Veterinary Parasitology 122, 293–301.CrossRefGoogle Scholar
Petkevičius, S., Nansen, P. and Bach Knudsen, K. E. ( 2000). The influence of insoluble carbohydrates on the populations of Ascaris suum and Oesophagostomum dentatum in pigs. Veterinarija ir Zootechnika 10, 15–17.Google Scholar
Petkevičius, S., Nansen, P., Bach Knudsen, K. E. and Skjøth, F. ( 1999). The effect of increasing levels of insoluble dietary fibre on the establishment and persistence of Oesophagostomum dentatum in pigs. Parasite 6, 17–26.CrossRefGoogle Scholar
Phelps, C. ( 1965). The physical properties of inulin solutions. Journal of Biochemistry 45, 41–47.CrossRefGoogle Scholar
Powers, K. G. ( 1959). Swine whipworm in Wisconsin. Veterinary Medicine 54, 396–397.Google Scholar
Robertfroid, M. B., Van Loo, J. A. E. and Gibson, G. R. ( 1998). The bifidogenic nature of chicory inulin and it's hydrolysis products. Journal of Nutrition 128, 11–19.Google Scholar
Roepstorff, A. and Nansen, P. ( 1998). Epidemiology, diagnosis and control of helminth parasites of swine. FAO Animal Health Manual No. 3. FAO, Rome, Italy.
Sobotka, L., Bratova, M., šlemrova, M., Manak, J., Vižda, J. and Zadak, Z. ( 1997). Inulin as the soluble fiber in liquid enteral nutrition. Nutrition 13, 21–25.CrossRefGoogle Scholar
Stewart, T. B. and Hale, O. M. ( 1988). Losses to internal parasites in swine production. Journal of Animal Science 66, 1548–1554.CrossRefGoogle Scholar
Thomsen, L. E., Petkevičius, S., Bach Knudsen, K. E. and Roepstorff, A. ( 2005). The influence of dietary carbohydrates on experimental infection with Trichuris suis in pigs. Parasitology 131, 857–865.CrossRefGoogle Scholar